CBFA2T3 (NM_005187) Human Untagged Clone

SKU SC314335

CBFA2T3 (untagged)-Human core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (CBFA2T3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CBFA2T3
• Synonyms: ETO2; MTG16; MTGR2; RUNX1T3; ZMYND4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005187 edited; ATGCCGGCTTCAAGACTGAGGGACAGGGCAGCCAGTTCAGCCTCGGGATCCACCTGTGGC; TCCATGTCCCAGACGCACCCTGTGCTGGAGAGCGGCCTCCTGGCATCTGCCGGCTGCTCC; GCACCCCGGGGTCCCAGGAAGGGCGGCCCAGCCCCAGTGGACAGGAAAGCTAAGGCCTCA; GCGATGCCGGACTCCCCAGCGGAGGTGAAGACGCAGCCCCGGTCCACACCCCCCAGCATG; CCGCCCCCACCGCCTGCCGCATCCCAGGGGGCCACACGCCCCCCCTCCTTCACGCCACAC; ACACATCGAGAGGACGGGCCTGCGACGCTGCCCCACGGCCGTTTTCATGGCTGCTTAAAA; TGGTCTATGGTCTGTCTCTTGATGAACGGCAGCAGCCACTCACCAACAGCCATCAATGGT; GCACCGTGCACACCCAACGGCTTCAGCAATGGCCCGGCCACCTCGTCCACAGCCTCCTTG; TCCACACAGCACCTGCCCCCAGCCTGCGGGGCCCGGCAGCTCAGCAAGCTCAAGCGCTTC; CTCACCACACTGCAGCAGTTTGGCAGCGACATCTCCCCAGAGATTGGGGAGCGCGTGCGC; ACACTGGTGCTGGGCCTGGTGAACTCGACATTGACGATCGAGGAGTTTCATTCCAAGCTT; CAGGAGGCCACCAACTTCCCTCTGCGGCCGCTTGTCATTCCCTTCCTGAAGGCAAACCTG; CCCTTGCTGCAGCGGGAGCTCCTGCACTGTGCACGCCTGGCCAAGCAGACGCCCGCCCAG; TACTTGGCCCAGCATGAGCAGCTCCTGCTGGACGCCAGCGCCTCCTCCCCCATCGACTCC; TCAGAGCTGCTACTGGAAGTCAACGAGAACGGCAAGAGGAGGACGCCCGACAGGACCAAA; GAGAACGGGTCAGACCGCGACCCGCTGCACCCCGAGCACCTCAGCAAACGGCCATGCACC; CTGAACCCTGCCCAGCGCTACAGCCCCAGCAACGGGCCACCGCAGCCCACACCGCCGCCG; CACTACCGCCTGGAGGACATAGCCATGGCCCACCACTTCCGAGATGCCTACCGCCACCCA; GACCCCCGGGAGCTACGAGAGCGCCATCGGCCGCTTGTGGTGCCTGGGTCCCGGCAGGAA; GAAGTGATCGACCACAAGCTCACAGAGCGTGAGTGGGCAGAAGAGTGGAAGCACCTCAAC; AACCTCCTGAACTGCATCATGGACATGGTGGAGAAGACGCGGCGCTCGCTCACGGTGCTG; CGCAGGTGCCAGGAGGCCGACCGCGAGGAGCTCAACCACTGGGCGCGGCGCTACAGCGAC; GCCGAGGACACAAAGAAGGGCCCCGCTCCCGCCGCGGCCCGGCCCCGCAGCAGCTCCGCC; GGTCCCGAAGGGCCTCAGCTAGACGTGCCTCGCGAGTTCCTGCCGAGGACCCTCACCGGC; TACGTGCCTGAGGACATCTGGAGGAAGGCTGAAGAGGCCGTGAATGAGGTGAAGCGGCAG; GCCATGTCGGAGCTGCAGAAAGCCGTGTCGGACGCGGAGCGCAAAGCGCACGAGCTCATC; ACCACGGAGCGTGCCAAGATGGAGCGGGCCCTGGCCGAGGCGAAGCGGCAGGCCTCCGAG; GACGCCCTGACGGTCATCAACCAGCAGGAGGACTCCAGCGAGAGCTGCTGGAACTGCGGG; CGGAAAGCCAGTGAGACGTGCAGCGGCTGCAACGCGGCACGCTACTGCGGGTCCTTCTGC; CAGCATCGGGACTGGGAGAAGCATCACCACGTGTGTGGCCAGAGCCTGCAGGGCCCCACA; GCCGTGGTGGCCGACCCGGTGCCTGGACCGCCCGAAGCCGCCCACAGCCTGGGCCCCTCC; CTGCCTGTGGGTGCTGCCAGCCCCAGCGAAGCCGGCTCTGCGGGGCCTTCTCGCCCCGGC; TCCCCCAGCCCACCTGGCCCACTGGACACCGTGCCCCGCTGA;
• Restriction Sites: Please inquire
• ACCN: NM_005187
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_005187.4.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005187.4, NP_005178.4
• RefSeq Size: 4265 bp
• RefSeq ORF: 1962 bp
• Locus ID: 863
• UniProt ID: O75081
• Cytogenetics: 16q24.3
• Domains: TAFH, zf-MYND
• Protein Families: Transcription Factors
• Summary: This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(16;21)(q24;q22) translocation is one of the less common karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. This gene is also a putative breast tumor suppressor. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]; Transcript Variant: This variant (1) represents the longer transcript, encodes the longer isoform (1) and is also known as MTG16a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.