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SUMF2 (NM_015411) Human Untagged Clone

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SKU
SC312863
SUMF2 (untagged)-Human sulfatase modifying factor 2 (SUMF2), transcript variant 2
  $330.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SUMF2
Synonyms pFGE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC312863 representing NM_015411.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGGGCGCATGCGCAGCGGGGCCGTGGGTGTACGCGGCGCAGCGCGGCAGTCCTGATGGCCCGGCAT
GGGTTACCGCTGCTGCCCCTGCTGTCGCTCCTGGTCGGCGCGTGGCTCAAGCTAGGAAATGGACAGGCT
ACTAGCATGGTCCAACTGCAGGGTGGGAGATTCCTGATGGGAACAAATTCTCCAGACAGCAGAGATGGT
GAAGGGCCTGTGCGGGAGGCGACAGTGAAACCCTTTGCCATCGACATATTTCCTGTCACCAACAAAGAT
TTCAGGGATTTTGTCAGGGAGAAAAAGTATCGGACAGAAGCTGAGATGTTTGGATGGAGCTTTGTCTTT
GAGGACTTTGTCTCTGATGAGCTGAGAAACAAAGCCACCCAGCCAATGAAGTCTGTACTCTGGTGGCTT
CCAGTGGAAAAGGCATTTTGGAGGCAGCCTGCAGGTCCTGGCTCTGGCATCCGAGAGAGACTGGAGCAC
CCAGTGTTACACGTGAGCTGGAATGACGCCCGTGCCTACTGTGCTTGGCGGGGAAAACGACTGCCCACG
GAGGAAGAGTGGGAGTTTGCCGCCCGAGGGGGCTTGAAGGGTCAAGTTTACCCATGGGGGAACTGGTTC
CAGCCAAACCGCACCAACCTGTGGCAGGGAAAGTTCCCCAAGGGAGACAAAGCTGAGGATGGCTTCCAT
GGAGTCTCCCCAGTGAATGCTTTCCCCGCCCAGAACAACTACGGGCTCTATGACCTCCTGGGGAACGTG
TGGGAGTGGACAGCATCACCGTACCAGGCTGCTGAGCAGGACATGCGCGTCCTCCGGGGGGCATCCTGG
ATCGACACAGCTGATGGCTCTGCCAATCACCGGGCCCGGGTCACCACCAGGATGGGCAACACTCCAGAT
TCAGCCTCAGACAACCTCGGTTTCCGCTGTGCTGCAGACGCAGGCCGGCCGCCAGGGGAGCTGTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_015411
Insert Size 963 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015411.2
RefSeq Size 2064 bp
RefSeq ORF 963 bp
Locus ID 25870
UniProt ID Q8NBJ7
Cytogenetics 7p11.2
Domains DUF323
MW 35.9 kDa
Summary The catalytic sites of sulfatases are only active if they contain a unique amino acid, C-alpha-formylglycine (FGly). The FGly residue is posttranslationally generated from a cysteine by enzymes with FGly-generating activity. The gene described in this record is a member of the sulfatase-modifying factor family and encodes a protein with a DUF323 domain that localizes to the lumen of the endoplasmic reticulum. This protein has low levels of FGly-generating activity but can heterodimerize with another family member - a protein with high levels of FGly-generating activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) represents the longest transcript and encodes isoform b. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation data.
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Documents
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"NM_015411" in other vectors (3)
SKU Description Size Price
RC229159 SUMF2 (Myc-DDK-tagged)-Human sulfatase modifying factor 2 (SUMF2), transcript variant 2 10 ug
$300.00
RG229159 SUMF2 (tGFP-tagged) - Human sulfatase modifying factor 2 (SUMF2), transcript variant 2 10 ug
$500.00
SC327794 SUMF2 (untagged)-Human sulfatase modifying factor 2 (SUMF2) transcript variant 2 10 ug
$630.00

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