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RALGDS (NM_001042368) Human Untagged Clone

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SKU
SC311316
RALGDS (untagged)-Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2
  $866.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RALGDS
Synonyms RalGEF; RGDS; RGF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC311316 representing NM_001042368.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGATGGTAGATTGCCAGAGCTCCACGCAGGAGATCGGTGAGGAGCTGATCAACGGAGTCATCTACTCC
ATCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGGCAGCGCTGGCTCGGGTATGAGAAT
GAGTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTGAAGGCTGGCACGCTGGAGAAGCTG
GTGGAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTCACCATCTTCCTGTGTACCTAT
AGAGCCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGGTACGGTAGATGTGACGCCCTC
ACGGCCTCCTCTAGATACGGCTGCATCCTCCCCTATTCCGACGAGGATGGTGGACCCCAGGACCAACTT
AAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTACTCGGAGGATTTCTGTCAACCTCCG
GACTTTCCCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAACATGCCAGGCTCAGACCTGGAGCGC
CGTGCCCACCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATTGAGGCAGAGCCTGAGGCTCTGTCA
CCAGTGCCAGCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTAACACCAGCTCGAGCACCCAGCCCA
GTGCCGGCTCCAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAGAGCTAGAAGTAGCT
CCAGCACCAGCTCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATCGGCTCCAGCGCCA
GCTCTGGAACTAGAGCCAGCTCCAGAACAGGATCCAGCTCCCTCACAAACTCTAGAGCTGGAGCCAGCT
CCAGCACCAGTTCCATCATTACAGCCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACGGGCTGAGTGAG
GAGAAGCCTCACCTCTTGGTGTTCCCTCCAGATCTGGTGGCAGAGCAGTTTACACTGATGGATGCGGAA
CTGTTCAAGAAGGTGGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAGAAGGGCAAG
GAGCACCTGGCGCCCACCATCCGCGCCACTGTCACCCAGTTCAACAGTGTGGCCAACTGTGTCATCACC
ACCTGCCTCGGGAACCGAAGCACGAAAGCCCCAGACAGGGCCAGGGTGGTGGAGCACTGGATCGAGGTG
GCCAGGGAGTGCCGGATCCTCAAGAACTTCTCGTCACTGTATGCCATCCTCTCTGCCCTGCAGAGCAAC
TCCATCCACCGTCTGAAGAAGACGTGGGAAGACGTTTCCAGGGACAGTTTCCGGATCTTTCAGAAGCTG
TCAGAGATCTTCTCAGATGAGAACAACTACTCATTGAGCCGGGAGCTGCTCATCAAGGAGGGCACCTCC
AAGTTTGCCACCCTGGAGATGAACCCCAAGAGAGCCCAGAAACGGCCGAAGGAGACGGGCATCATCCAG
GGCACCGTTCCCTACCTGGGCACGTTCCTCACCGACCTGGTGATGCTGGACACTGCCATGAAGGACTAT
CTGTATGGCAGACTCATCAACTTTGAGAAGAGGAGGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCTG
CTGCAGTCGGCCTGCAACAACTACAGCATCGCGCCAGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTG
GAGCGGCTCAGCGAGACTGAGAGCTACAACCTGTCGTGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGC
AACACCCTCAGGACCAAGAAGAACACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCCCCCAGCACT
GAGCTCAGTACCAGTGGCAGCTCCCACTCCAAGTCCTGTGACCAGCTCAGGTGTGGCCCCTACCTCAGC
AGCGGGGACATCGCTGACGCGCTCAGCGTGCACTCGGCCGGCTCCTCTAGCTCCGACGTGGAGGAGATC
AACATCAGCTTCGTCCCGGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAATCAGCCTCACAGTCA
TCCCCGGAGACCTCCGGCATCAGCTCAGCCTCCAGCAGCACCTCGTCCTCCTCAGCCTCCACCACGCCC
GTGGCTGCCACACGCACCCACAAGCGCTCTGTCTCAGGGCTCTGCAACTCCAGCTCCGCGCTGCCGCTC
TACAACCAGCAGGTGGGCGACTGCTGTATCATCCGCGTCAGCCTGGACGTGGACAATGGCAACATGTAC
AAGAGCATCCTGGTGACCAGCCAAGATAAGGCTCCGGCTGTAATCCGCAAGGCCATGGACAAACACAAC
CTGGAGGAGGAGGAGCCGGAGGACTATGAGCTGCTGCAGATTCTCTCAGATGACCGGAAGCTGAAGATC
CCTGAAAACGCCAACGTCTTCTATGCCATGAACTCTACCGCCAACTATGACTTTGTCCTCAAGAAGCGG
ACCTTCACCAAGGGAGTGAAGGTCAAGCACGGAGCCAGCTCCACCCTCCCTCGCATGAAGCAGAAAGGA
CTCAAGATTGCCAAGGGCATCTTCTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001042368
Insert Size 2580 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001042368.2
RefSeq Size 3709 bp
RefSeq ORF 2580 bp
Locus ID 5900
UniProt ID Q12967
Cytogenetics 9q34.13-q34.2
Protein Pathways Colorectal cancer, Pancreatic cancer, Pathways in cancer
MW 94.7 kDa
Summary Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.supplied by OMIM, Nov 2010
Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region and represents use of an alternate promoter, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.
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Documents
Resources
"NM_001042368" in other vectors (4)
SKU Description Size Price
RC222398 RALGDS (Myc-DDK-tagged)-Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2 10 ug
$786.00
RC222398L3 Lenti ORF clone of Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2, Myc-DDK-tagged 10 ug
$1,086.00
RC222398L4 Lenti ORF clone of Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2, mGFP tagged 10 ug
$1,086.00
RG222398 RALGDS (tGFP-tagged) - Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2 10 ug
$986.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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