RTN4RL2 (NM_178570) Human Untagged Clone

SKU SC310413

RTN4RL2 (untagged)-Human reticulon 4 receptor-like 2 (RTN4RL2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RTN4RL2
• Synonyms: NgR2; NGRH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310413 representing NM_178570.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCTGCCCGGGCTCAGGCGCCTGCTGCAAGCTCCCGCCTCGGCCTGCCTCCTGCTGATGCTCCTGGCC; CTGCCCCTGGCGGCCCCCAGCTGCCCCATGCTCTGCACCTGCTACTCATCCCCGCCCACCGTGAGCTGC; CAGGCCAACAACTTCTCCTCTGTGCCGCTGTCCCTGCCACCCAGCACTCAGCGACTCTTCCTGCAGAAC; AACCTCATCCGCACGCTGCGGCCAGGCACCTTTGGGTCCAACCTGCTCACCCTGTGGCTCTTCTCCAAC; AACCTCTCCACCATCTACCCGGGCACTTTCCGCCACTTGCAAGCCCTGGAGGAGCTGGACCTCGGTGAC; AACCGGCACCTGCGCTCGCTGGAGCCCGACACCTTCCAGGGCCTGGAGCGGCTGCAGTCGCTGCATTTG; TACCGCTGCCAGCTCAGCAGCCTGCCCGGCAACATCTTCCGAGGCCTGGTCAGCCTGCAGTACCTCTAC; CTCCAGGAGAACAGCCTGCTCCACCTACAGGATGACTTGTTCGCGGACCTGGCCAACCTGAGCCACCTC; TTCCTCCACGGGAACCGCCTGCGGCTGCTCACAGAGCACGTGTTTCGCGGCCTGGGCAGCCTGGACCGG; CTGCTGCTGCACGGGAACCGGCTGCAGGGCGTGCACCGCGCGGCCTTCCGCGGCCTCAGCCGCCTCACC; ATCCTCTACCTGTTCAACAACAGCCTGGCCTCGCTGCCCGGCGAGGCGCTCGCCGACCTGCCCTCGCTC; GAGTTCCTGCGGCTCAACGCTAACCCCTGGGCGTGCGACTGCCGCGCGCGGCCGCTCTGGGCCTGGTTC; CAGCGCGCGCGCGTGTCCAGCTCCGACGTGACCTGCGCCACCCCCCCGGAGCGCCAGGGCCGAGACCTG; CGCGCGCTCCGCGAGGCCGACTTCCAGGCGTGTCCGCCCGCGGCACCCACGCGGCCGGGCAGCCGCGCC; CGCGGCAACAGCTCCTCCAACCACCTGTACGGGGTGGCCGAGGCCGGGGCGCCCCCAGCCGATCCCTCC; ACCCTCTACCGAGATCTGCCTGCCGAAGACTCGCGGGGGCGCCAGGGCGGGGACGCGCCTACTGAGGAC; GACTACTGGGGGGGCTACGGGGGTGAGGACCAGCGAGGGGAGCAGATGTGCCCCGGCGCTGCCTGCCAG; GCGCCCCCGGACTCCCGAGGCCCTGCGCTCTCGGCCGGGCTCCCCAGCCCTCTGCTTTGCCTCCTGCTC; CTGGTGCCCCACCACCTCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_178570
• Insert Size: 1263 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178570.2
• RefSeq Size: 2220 bp
• RefSeq ORF: 1263 bp
• Locus ID: 349667
• UniProt ID: Q86UN3
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome
• MW: 46.1 kDa
• Summary: Cell surface receptor that plays a functionally redundant role in the inhibition of neurite outgrowth mediated by MAG (By similarity). Plays a functionally redundant role in postnatal brain development. Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum. Does not seem to play a significant role in regulating axon regeneration in the adult central nervous system. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG (By similarity). Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed:22325200). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed:22325200).[UniProtKB/Swiss-Prot Function]