TRIM16 (NM_006470) Human Untagged Clone

SKU
SC310262
TRIM16 (untagged)-Human tripartite motif containing 16 (TRIM16)
  $578.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TRIM16
Synonyms EBBP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC310262 representing NM_006470.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTGAGTTGGATCTAATGGCTCCAGGGCCACTGCCCAGGGCCACTGCTCAGCCCCCAGCCCCTCTC
AGCCCAGACTCTGGGTCACCCAGCCCAGATTCTGGGTCAGCCAGCCCAGTGGAAGAAGAGGACGTGGGC
TCCTCGGAGAAGCTTGGCAGGGAGACGGAGGAACAGGACAGCGACTCTGCAGAGCAGGGGGATCCTGCT
GGTGAGGGGAAAGAGGTCCTGTGTGACTTCTGCCTTGATGACACCAGAAGAGTGAAGGCAGTGAAGTCC
TGTCTAACCTGCATGGTGAATTACTGTGAAGAGCACTTGCAGCCGCATCAGGTGAACATCAAACTGCAA
AGCCACCTGCTGACCGAGCCAGTGAAGGACCACAACTGGCGATACTGCCCTGCCCACCACAGCCCACTG
TCTGCCTTCTGCTGCCCTGATCAGCAGTGCATCTGCCAGGACTGTTGCCAGGAGCACAGTGGCCACACC
ATAGTCTCCCTGGATGCAGCCCGCAGGGACAAGGAGGCTGAACTCCAGTGCACCCAGTTAGACTTGGAG
CGGAAACTCAAGTTGAATGAAAATGCCATCTCCAGGCTCCAGGCTAACCAAAAGTCTGTTCTGGTGTCG
GTGTCAGAGGTCAAAGCGGTGGCTGAAATGCAGTTTGGGGAACTCCTTGCTGCTGTGAGGAAGGCCCAG
GCCAATGTGATGCTCTTCTTAGAGGAGAAGGAGCAAGCTGCGCTGAGCCAGGCCAACGGTATCAAGGCC
CACCTGGAGTACAGGAGTGCCGAGATGGAGAAGAGCAAGCAGGAGCTGGAGAGGATGGCGGCCATCAGC
AACACTGTCCAGTTCTTGGAGGAGTACTGCAAGTTTAAGAACACTGAAGACATCACCTTCCCTAGTGTT
TACGTAGGGCTGAAGGATAAACTCTCGGGCATCCGCAAAGTTATCACGGAATCCACTGTACACTTAATC
CAGTTGCTGGAGAACTATAAGAAAAAGCTCCAGGAGTTTTCCAAGGAAGAGGAGTATGACATCAGAACT
CAAGTGTCTGCCGTTGTTCAGCGCAAATATTGGACTTCCAAACCTGAGCCCAGCACCAGGGAACAGTTC
CTCCAATATGCGTATGACATCACGTTTGACCCGGACACAGCACACAAGTATCTCCGGCTGCAGGAGGAG
AACCGCAAGGTCACCAACACCACGCCCTGGGAGCATCCCTACCCGGACCTCCCCAGCAGGTTCCTGCAC
TGGCGGCAGGTGCTGTCCCAGCAGAGTCTGTACCTGCACAGGTACTATTTTGAGGTGGAGATCTTCGGG
GCAGGCACCTATGTTGGCCTGACCTGCAAAGGCATCGACCGGAAAGGGGAGGAGCGCAACAGTTGCATT
TCCGGAAACAACTTCTCCTGGAGCCTCCAATGGAACGGGAAGGAGTTCACGGCCTGGTACAGTGACATG
GAGACCCCACTCAAAGCTGGCCCTTTCCGGAGGCTCGGGGTCTATATCGACTTCCCGGGAGGGATCCTT
TCCTTCTATGGCGTAGAGTATGATACCATGACTCTGGTTCACAAGTTTGCCTGCAAATTTTCAGAACCA
GTCTATGCTGCCTTCTGGCTTTCCAAGAAGGAAAACGCCATCCGGATTGTAGATCTGGGAGAGGAACCC
GAGAAGCCAGCACCGTCCTTGGTGGGGACTGCTCCCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_006470
Insert Size 1695 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006470.3
RefSeq Size 2920 bp
RefSeq ORF 1695 bp
Locus ID 10626
UniProt ID O95361
Cytogenetics 17p12
Domains PRY, SPRY, zf-B_box
Protein Families Druggable Genome, Transcription Factors
MW 64 kDa
Summary The protein encoded by this gene is a tripartite motif (TRIM) family member that contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. While it lacks a RING domain found in other TRIM proteins, the encoded protein can homodimerize or heterodimerize with other TRIM proteins and has E3 ubiquitin ligase activity. This gene is also a tumor suppressor and is involved in secretory autophagy. provided by RefSeq, Jan 2017
Transcript Variant: This variant (3), as well as variants 1 and 2, encodes isoform a.
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Documents
Resources
"NM_006470" in other vectors (4)
SKU Description Size Price
RC208564 TRIM16 (Myc-DDK-tagged)-Human tripartite motif containing 16 (TRIM16) 10 ug
$525.00
RC208564L3 Lenti ORF clone of Human tripartite motif containing 16 (TRIM16), Myc-DDK-tagged 10 ug
$825.00
RC208564L4 Lenti ORF clone of Human tripartite motif containing 16 (TRIM16), mGFP tagged 10 ug
$825.00
RG208564 TRIM16 (tGFP-tagged) - Human tripartite motif containing 16 (TRIM16) 10 ug
$725.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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