NADPH oxidase 4 (NOX4) (NM_016931) Human Untagged Clone

SKU SC310253

NOX4 (untagged)-Human NADPH oxidase 4 (NOX4), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NADPH oxidase 4
• Synonyms: KOX; KOX-1; RENOX
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016931 edited; CCGCTGCCCCGGCCGCCGCCCGCTCCTTCTCGGTCCGGCGGGCACAGAGCGCAGCGCGGC; GGGGCCGGCG GCATGGCTGTGTCCTGGAGGAGCTGGCTCGCCAACGAAGGGGTTAAACA; CCTCTGCCTGTTCATCTGGCT CTCCATGAATGTCCTGCTTTTCTGGAAAACCTTCTTGC; TGTATAACCAAGGGCCAGAGTATCACTACCTC CACCAGATGTTGGGGCTAGGATTGTGT; CTAAGCAGAGCCTCAGCATCTGTTCTTAACCTCAACTGCAGCC TTATCCTTTTACCCAT; GTGCCGAACACTCTTGGCTTACCTCCGAGGATCACAGAAGGTTCCAAGCAGGAG AACCA; GGAGATTGTTGGATAAAAGCAGAACATTCCATATTACCTGTGGTGTTACTATCTGTATTT; TCTCA GGCGTGCATGTGGCTGCCCATCTGGTGAATGCCCTCAACTTCTCAGTGAATTAC; AGTGAAGACTTTGTTG AACTGAATGCAGCAAGATACCGAGATGAGGATCCTAGAAAACT; TCTCTTCACAACTGTTCCTGGCCTGAC AGGGGTCTGCATGGTGGTGGTGCTATTCCTCA; TGATCACAGCCTCTACATATGCAATAAGAGTTTCTAAC TATGATATCTTCTGGTATACT; CATAACCTCTTCTTTGTCTTCTACATGCTGCTGACGTTGCATGTTTCAG GAGGGCTGCT; GAAGTATCAAACTAATTTAGATACCCACCCTCCCGGCTGCATCAGTCTTAACCGAACCAG; CTCTCAGAATATTTCCTTACCAGAGTATTTCTCAGAACATTTTCATGAACCTTTCCCTG; AAGGATTTTCA AAACCGGCAGAGTTTACCCAGCACAAATTTGTGAAGATTTGTATGGAA; GAGCCCAGATTCCAAGCTAATT TTCCACAGACTTGGCTTTGGATTTCTGGACCTTTGTG; CCTGTACTGTGCCGAAAGACTTTACAGGTATAT CCGGAGCAATAAGCCAGTCACCATCA; TTTCGGTCATAAGTCATCCCTCAGATGTCATGGAAATCCGAATG GTCAAAGAAAATTTT; AAAGCAAGACCTGGTCAGTATATTACTCTACATTGTCCCAGTGTATCTGCATTAG AAAA; TCATCCATTTACCCTCACAATGTGTCCAACTGAAACCAAAGCAACATTTGGGGTTCATCT; TAAAAT AGTAGGAGACTGGACAGAACGATTTCGAGATTTACTACTGCCTCCATCTAGTC; AAGACTCCGAAATTCTG CCCTTCATTCAATCTAGAAATTATCCCAAGCTGTATATTGAT; GGTCCTTTTGGAAGTCCATTTGAGGAAT CACTGAACTATGAGGTCAGCCTCTGCGTGGC; TGGAGGCATTGGAGTAACTCCATTTGCATCAATACTCAA CACCCTGTTGGATGACTGGA; AACCATACAAGCTTAGAAGACTATACTTTATTTGGGTATGCAGAGATATC CAGTCCTTC; CGTTGGTTTGCAGATTTACTCTGTATGTTGCATAACAAGTTTTGGCAAGAGAACAGACCT; G ACTATGTCAACATCCAGCTGTACCTCAGTCAAACAGATGGGATACAGAAGATAATTGG; AGAAAAATATCA TGCACTGAATTCAAGACTGTTTATAGGACGTCCTCGGTGGAAACTTT; TGTTTGATGAAATAGCAAAATAT AACAGAGGAAAAACAGTTGGTGTTTTCTGTTGTGGA; CCCAATTCACTATCCAAGACTCTTCATAAACTGA GTAACCAGAACAACTCATATGGGAC; AAGATTTGAATACAATAAAGAGTCTTTCAGCTGAAAACTTTTGCC ATGAAGCAGGACTC; TAAAGAAGGAATGAGTGCAATTTCTAAGACTTTGAAACTCAGCGGAATCAATCAGC TGT; GTTATGCCAAAGAATAGTAAGGTTTTCTTATTTATGATTATTTAAAATGGAAATGTGAGA; ATGTGGC AAGATGACCGTCACATTACATGTTTAATCTGGAAACCAAAGAGACCCTGAAG; AATATTTGATGTGATGAT TCACTTTTCAGTTCTCAAATTAAAAGAAAACTGTTAGATGC; AAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016931 unedited; NNGGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCAATCTGGTACCGGT; CCGGAATTCCCGGGATCCGCTGCCCCGGCCGCCGCCCGCTCCTTCTCGGTCCGGCGGGCA; CAGAGCGCAGCGCGGCGGGGCCGGCGGCATGGCTGTGTCCTGGAGGAGCTGGCTCGCCAA; CGAAGGGGTTAAACACCTCTGCCTGTTCATCTGGCTCTCCATGAATGTCCTGCTTTTCTG; GAAAACCTTCTTGCTGTATAACCAAGGGCCAGAGTATCACTACCTCCACCAGATGTTGGG; GCTAGGATTGTGTCTAAGCAGAGCCTCAGCATCTGTTCTTAACCTCAACTGCAGCCTTAT; CCTTTTACCCATGTGCCGAACACTCTTGGCTTACCTCCGAGGATCACAGAAGGTTCCAAG; CAGGAGAACCAGGAGATTGTTGGATAAAAGCAGAACATTCCATATTACCTGTGGTGTTAC; TATCTGTATTTTCTCAGGCGTGCATGTGGCTGCCCATCTGGTGAATGCCCTCAACTTCTC; AGTGAATTACAGTGAAGACTTTGTTGAACTGAATGCAGCAAGATACCGAGATGAGGATCC; TAGAAAACTTCTCTTCACAACTGTTCCTGGCCTGACAGGGGTCTGCATGGTGGTGGTGCT; ATTCCTCATGATCACAGCCTCTACATATGCAATAAGAGTTTCTAACTATGATATCTTCTG; GTATACTCATAACCTCTTCTTTGTCTTCTACATGCTGCTGACGTTGCATGTTTCANGAGG; GCTGCTGAAGTATCAAACTAATTTAGATACCCACCCTCCCGGCTGCATCAGTCTTAACCG; AACCAGCTCTCAGATATTTCCTTACCAGAGTATTTCTCAGAACATTTTCATGAACCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016931 unedited; AAGAGCAATGGGGAGGGTCAAGGGNNTAGCACCGGGTATGTTCAGAAAAGCTATGACCGC; GGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCATCTAACAGTTNN; TTCTTTTTAATTTGAGAACTGAAAAGTGAATCATCACATCAAATATTCTTCAGGGTCTCT; TTGGTTTCCAGATTAAACATGTAATGTGACGGTCATCTTGCCACATTCTCACATTTCCAT; TTTAAATAATCATAAATAAGAAAACCTTACTATTCTTTGGCATAACACAGCTGATTGATT; CCGCTGAGTTTCAAAGTCTTAGAAATTGCACTCATTCCTTCTTTAGAGTCCTGCTTCATG; GCAAAAGTTTTCAGCTGAAAGACTCTTTATTGTATTCAAATCTTGTCCCATATGAGTTGT; TCTGGTTACTCAGTTTATGAAGAGTCTGNATAGTGAATTTGGGTCCACAACAGAAACACC; AACTGGTTTTCCTCTGTTATATTTTGCTATTTCATCAAACAAAAGTTTCCACCCAGAACC; TCCATTAACAGTCTTTGAATTCGTGCCTGAATTTTTTTTTTCCATTATCTTTTTGTTTCC; CATCTGTTTTAACTGAAGGTACCACTGGGTTTTTGAACTTAATCCGGGTTTTGTTTTCTT; TGGCAAACTTTGTTTTTGCCAACATCAACGAGTTAAATCCGGCAACACCACCGGGAGGGC; TTGGGTTTTTTCTTGCTTTCCCCCAAAAAAAGTTTAATCTTTTTCAAACTTGGTTGGGGG; TTTCCAGTTCACCCCCACGGGGTTGTTGAGTTTTTTGTGGCCCAAAGGGGGTTTTTTCCC; CAAGGGCCCTCCCCCCCCCCCAGAGGTTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_016931
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016931.2, NP_058627.1
• RefSeq Size: 2416 bp
• RefSeq ORF: 1737 bp
• Locus ID: 50507
• UniProt ID: Q9NPH5
• Cytogenetics: 11q14.3
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]; Transcript Variant: This variant (1) encodes isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.