RASA4 (NM_006989) Human Untagged Clone

SKU SC310102

RASA4 (untagged)-Human RAS p21 protein activator 4 (RASA4), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RASA4
• Synonyms: CAPRI; GAPL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310102 representing NM_006989.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCCAAGCGCAGCTCGCTGTACATCCGCATCGTGGAGGGGAAGAACCTTCCCGCCAAGGACATCACT; GGCAGCAGCGACCCCTACTGCATCGTGAAGGTGGACAATGAGCCCATCATCAGGACAGCCACAGTGTGG; AAGACCCTGTGCCCCTTCTGGGGTGAGGAGTACCAAGTGCACCTGCCGCCCACCTTCCACGCTGTGGCT; TTCTATGTCATGGATGAGGATGCCCTCAGCCGGGACGACGTTATCGGAAAGGTCTGCCTTACAAGGGAC; ACCATAGCCTCTCACCCTAAGGGTTTCAGCGGGTGGGCCCACCTGACGGAGGTCGACCCCGATGAGGAG; GTGCAGGGCGAGATCCACCTGCGGCTGGAAGTGTGGCCAGGGGCCCGGGCCTGCCGGCTACGCTGCTCT; GTGCTGGAGGCCAGGGATCTGGCCCCAAAGGACCGCAATGGCACATCTGACCCCTTCGTCCGAGTGCGC; TACAAGGGCCGGACACGGGAGACCTCGATCGTGAAGAAGTCATGCTACCCACGCTGGAATGAGACGTTT; GAATTTGAGCTGCAGGAGGGGGCCATGGAGGCGCTGTGCGTGGAGGCCTGGGACTGGGACCTTGTCAGC; CGAAACGACTTCCTGGGCAAAGTGGTGATTGATGTCCAGAGACTGCGGGTGGTGCAGCAGGAGGAGGGC; TGGTTCCGGCTGCAGCCCGACCAGTCCAAGAGCCGGCGGCATGACGAGGGCAACCTGGGCTCCTTGCAG; CTGGAGGTGCGGCTGCGGGACGAGACGGTGCTGCCCTCCAGCTACTACCAGCCACTGGTGCACCTGCTG; TGCCACGAGGTCAAGCTGGGCATGCAGGGCCCAGGGCAGCTGATCCCACTCATCGAGGAGACAACCAGC; ACCGAGTGTCGCCAGGACGTGGCCACGAACCTGCTCAAGCTCTTCCTGGGGCAGGGGCTGGCCAAGGAC; TTCCTGGACCTGCTCTTCCAGCTGGAGCTGAGTCGCACCAGTGAGACCAACACCCTGTTCCGGAGCAAC; TCTCTGGCCTCAAAGTCCATGGAGTCTTTTCTGAAGGTGGCCGGGATGCAGTACCTGCACGGCGTCCTG; GGCCCCATCATCAACAAGGTGTTTGAGGAGAAGAAGTACGTGGAGCTGGACCCCAGCAAAGTGGAAGTT; AAGGATGTAGGGTGCTCCGGGCTGCACCGCCCGCAGACCGAGGCCGAGGTGCTGGAGCAGAGCGCGCAG; ACGCTGCGCGCCCACCTGGGGGCCCTGCTGAGCGCGCTCAGCCGCTCGGTTCGCGCGTGCCCCGCCGTG; GTGCGCGCCACCTTCCGCCAGCTCTTCCGGCGCGTGCGCGAGCGCTTCCCCGGCGCCCAGCACGAGAAT; GTACCGTTCATCGCCGTCACCAGCTTCCTGTGCCTGCGCTTCTTCTCTCCCGCCATCATGTCGCCCAAG; CTCTTCCACCTGCGGGAGCGCCACGCGGACGCCCGCACCAGCCGCACCCTGCTCCTGTTGGCCAAGGCA; GTCCAGAACGTGGGCAACATGGACACGCCGGCTTCCAGGGCCAAGGAGGCTTGGATGGAGCCGCTGCAG; CCCACCGTGCGCCAGGGCGTGGCGCAGCTGAAGGACTTCATCACCAAGCTCGTGGACATCGAGGAGAAG; GACGAGCTGGACCTGCAGCGGACGCTGAGTTTGCAGGCGCCACCTGTGAAGGAGGGGCCACTCTTCATC; CACAGGACCAAGGGCAAGGGCCCCCTCATGTCCTCCTCCTTCAAGAAGCTCTACTTCTCCCTCACTACC; GAGGCCCTCAGCTTCGCGAAGACGCCCAGCTCCAAGAAAAGCGCCCTCATCAAGTTAGCCAACATCCGG; GCAGCGGAAAAGGTTGAGGAAAAGAGCTTTGGCGGCTCGCACGTCATGCAGGTCATCTACACGGACGAC; GCCGGCAGGCCCCAGACTGCCTACCTGCAGTGCAAGTGTGTGAATGAGCTTAACCAGTGGCTGTCTGCG; CTGCGGAAGGTGAGCATCAACAACACCGGACTGCTGGGCTCCTACCACCCTGGCGTCTTCCGTGGGGAC; AAGTGGAGCTGCTGCCACCAAAAAGAGAAGACAGGTCAGGGCTGCGATAAGACCCGGTCACGGGTGACC; CTGCAGGAGTGGAATGACCCTCTTGACCATGACCTTGAGGCCCAGCTCATCTACCGGCACCTGCTGGGC; GTGGAGGCCATGCTGTGGGAGAGGCACCGGGAGCTGAGCGGGGGCGCAGAGGCAGGCACGGTGCCCACG; AGCCCTGGCAAAGTCCCCGAGGACTCATTGGCCCGGCTGCTCCGGGTGCTGCAGGACCTCCGCGAGGCC; CATAGCTCCAGCCCGGCCGGCTCCCCACCCTCAGAGCCCAACTGCCTCCTGGAGCTGCAGACGTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_006989
• Insert Size: 2412 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006989.5
• RefSeq Size: 5606 bp
• RefSeq ORF: 2412 bp
• Locus ID: 10156
• UniProt ID: O43374
• Cytogenetics: 7q22.1
• Domains: BTK, C2, PH, RasGAP
• Protein Families: Druggable Genome
• MW: 90.5 kDa
• Summary: This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.