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IFT172 (NM_015662) Human Untagged Clone

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SKU
SC309828
IFT172 (untagged)-Human intraflagellar transport 172 homolog (Chlamydomonas) (IFT172)
  $1,617.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol IFT172
Synonyms BBS20; NPHP17; osm-1; RP71; SLB; SRTD10; wim
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC309828 representing NM_015662.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCACTTGAAGCACCTGAGGACCCTGCTGAGCCCTCAGGATGGAGCTGCAAAGGTGACCTGCATGGCT
TGGTCCCAGAACAATGCCAAATTTGCTGTCTGCACAGTGGACCGAGTGGTCTTGCTGTATGATGAACAT
GGAGAACGGAGAGATAAATTCTCCACCAAACCAGCTGACATGAAGTATGGCAGGAAGAGCTATATGGTG
AAGGGCATGGCTTTTTCTCCTGATTCCACTAAAATTGCCATAGGACAGACTGACAACATCATCTATGTC
TACAAGATTGGAGAAGATTGGGGTGACAAGAAAGTCATCTGCAACAAGTTCATCCAGACGAGTGCTGTC
ACTTGTCTGCAATGGCCGGCAGAATACATCATTGTCTTTGGACTGGCTGAAGGGAAGGTTCGTTTAGCA
AACACCAAAACTAATAAATCATCTACCATCTATGGGACAGAGTCTTACGTGGTGTCCCTGACAACAAAT
TGCTCTGGGAAAGGAATTCTCTCTGGTCATGCAGATGGTACCATCGTTAGGTATTTCTTTGATGATGAA
GGCTCTGGAGAGTCACAGGGGAAGTTGGTTAACCACCCGTGTCCACCCTATGCCTTGGCATGGGCAACC
AATAGCATCGTGGCTGCAGGCTGTGATCGGAAAATTGTAGCCTATGGAAAAGAAGGTCACATGCTACAA
ACTTTTGATTATAGCCGTGACCCTCAGGAGCGGGAGTTCACCACAGCTGTATCAAGTCCTGGGGGCCAG
TCTGTTGTGCTAGGAAGTTATGACAGGCTTCGGGTGTTCAACTGGATCCCTCGAAGAAGCATCTGGGAA
GAGGCAAAGCCCAAGGAGATTACCAATTTATACACCATCACTGCCTTGGCCTGGAAGCGGGATGGCTCA
CGGCTCTGTGTGGGCACACTATGTGGTGGGGTGGAACAGTTTGACTGCTGCCTCCGAAGGAGTATTTAC
AAGAACAAGTTTGAGTTGACGTATGTGGGACCTAGCCAGGTGATTGTGAAGAACCTGTCATCAGGAACC
CGAGTGGTGCTCAAGTCACACTATGGCTATGAGGTGGAAGAGGTGAAAATCCTAGGAAAGGAACGTTAC
TTGGTGGCTCACACATCAGAAACACTGCTGCTGGGGGACCTGAACACTAATCGGCTTAGTGAGATAGCC
TGGCAAGGATCTGGTGGCAATGAGAAGTATTTCTTTGAAAATGAGAATGTATGCATGATCTTCAATGCC
GGAGAGCTAACCCTGGTGGAATATGGGAATAATGACACCCTGGGTTCTGTACGCACTGAATTCATGAAC
CCCCACCTCATCAGTGTTCGTATTAATGAGAGGTGTCAGCGAGGAACAGAAGATAATAAGAAATTGGCT
TATCTTATTGATATTAAGACTATTGCTATAGTGGATCTGATTGGTGGCTACAACATTGGCACCGTCAGC
CATGAGAGCCGTGTGGATTGGCTGGAACTTAATGAGACTGGACACAAGCTCCTCTTCAGGGACCGGAAA
CTTCGTTTGCATCTGTATGATATTGAAAGCTGCTCTAAGACAATGATCCTCAACTTCTGCTCCTATATG
CAGTGGGTCCCAGGAAGTGACGTGCTGGTAGCTCAGAACCGAAACAGTCTGTGTGTATGGTACAACATT
GAGGCACCTGAGAGAGTCACCATGTTCACTATTAGGGGTGATGTTATAGGTCTGGAGCGGGGCGGGGGA
AAGACCGAGGTGATGGTGATGGAAGGTGTGACTACTGTTGCCTACACATTGGATGAGGGCCTCATCGAG
TTTGGAACAGCCATTGATGATGGCAACTACATCCGGGCAACAGCCTTCTTAGAGACTCTGGAAATGACC
CCAGAAACAGAGGCAATGTGGAAAACCTTGAGTAAACTGGCACTAGAGGCAAGGCAACTACACATTGCG
GAGAGGTGCTTTTCTGCTTTGGGCCAAGTAGCAAAAGCTCGATTCCTGCATGAGACCAATGAGATTGCA
GATCAAGTATCCCGGGAATATGGCGGAGAAGGAACAGACTTTTATCAGGTCCGAGCACGTCTAGCCATG
CTGGAAAAGAACTACAAACTGGCTGAAATGATCTTTTTGGAACAGAATGCTGTGGAGGAGGCCATGGGC
ATGTACCAGGAGCTACACCGTTGGGATGAGTGTATCGCTGTGGCTGAAGCCAAGGGGCACCCAGCCCTG
GAGAAGCTACGTCGTAGTTACTACCAGTGGCTGATGGACACACAGCAAGAGGAGCGAGCAGGTGAACTA
CAGGAGAGCCAAGGGGATGGGCTAGCAGCCATCAGCCTCTACCTCAAAGCTGGGCTCCCTGCCAAAGCT
GCTCGGCTGGTGCTGACCCGAGAGGAACTGCTAGCCAACACAGAGCTGGTAGAACACATCACTGCAGCC
CTTATCAAGGGGGAACTCTACGAAAGGGCAGGTGATCTCTTTGAGAAGATTCACAATCCACAGAAGGCC
CTGGAGTGCTACCGTAAAGGCAACGCATTCATGAAAGCGGTAGAGCTGGCTCGATTGGCCTTCCCAGTG
GAGGTGGTGAAACTAGAGGAGGCATGGGGGGACCACCTGGTGCAGCAGAAGCAGCTTGATGCAGCCATT
AATCACTACATCGAAGCCAGGTGCTCCATTAAGGCAATTGAGGCCGCCCTGGGTGCCCGCCAGTGGAAG
AAGGCAATTTATATATTAGATCTACAGGACCGGAACACTGCATCCAAATACTATCCTCTCGTGGCCCAA
CACTATGCATCCCTGCAGGAGTATGAGATTGCTGAGGAGCTCTATACTAAGGGAGATCGGACAAAAGAT
GCCATAGACATGTACACCCAGGCTGGTCGTTGGGAACAAGCCCACAAGCTGGCGATGAAATGCATGAGA
CCAGAAGATGTGTCAGTGCTATACATCACTCAGGCCCAGGAAATGGAGAAGCAGGGCAAGTACCGTGAG
GCTGAAAGGCTATATGTGACAGTACAAGAGCCTGATCTTGCCATCACCATGTACAAAAAGCACAAGTTG
TATGATGACATGATCCGCCTGGTAGGGAAGCACCATCCAGATCTCCTCAGTGATACACACCTACATCTG
GGCAAGGAGCTGGAGGCTGAAGGCCGACTACAGGAGGCTGAGTACCACTACCTCGAGGCCCAGGAATGG
AAGGCAACAGTGAACATGTACCGGGCCAGTGGGCTTTGGGAAGAGGCCTACAGGGTGGCCAGAACTCAA
GGAGGGGCTAATGCCCACAAACACGTGGCCTATCTGTGGGCAAAGAGCCTGGGAGGAGAGGCTGCAGTT
AGACTGCTTAATAAGCTGGGACTCCTGGAAGCTGCTGTTGACCACGCTGCAGACAATTGCTCCTTTGAA
TTTGCGTTTGAACTCTCTCGGCTGGCCCTCAAGCACAAAACCCCCGAGGTTCATCTCAAATATGCTATG
TTCCTGGAGGATGAGGGTAAATTCGAAGAGGCTGAAGCTGAATTCATCAGAGCTGGTAAACCCAAGGAG
GCAGTCCTCATGTTTGTCCATAACCAGGATTGGGAGGCAGCTCAGCGTGTGGCTGAGGCTCACGACCCT
GACAGTGTCGCCGAGGTGCTTGTGGGACAGGCCCGGGGGGCCTTGGAGGAGAAGGACTTTCAGAAAGCA
GAAGGGCTGCTGCTCCGGGCCCAGAGACCAGGCCTGGCCCTCAATTATTATAAGGAGGCTGGATTATGG
AGTGACGCTCTGCGCATCTGCAAGGACTATGTGCCCAGCCAGCTGGAGGCTCTGCAGGAAGAATATGAG
CGGGAAGCTACTAAGAAGGGGGCCAGGGGTGTGGAGGGATTTGTGGAACAAGCTCGACACTGGGAGCAG
GCTGGAGAGTACAGCCGTGCCGTGGACTGCTACCTCAAAGTGCGAGACTCTGGAAACAGCGGCCTGGCG
GAGAAGTGCTGGATGAAGGCAGCTGAACTCTCCATCAAGTTTCTGCCTCCCCAACGTAATATGGAAGTC
GTTCTGGCTGTAGGACCCCAGCTGATTGGAATTGGAAAGCACAGTGCAGCTGCAGAGCTCTATCTGAAT
CTGGACCTTGTCAAGGAAGCAATCGATGCTTTCATCGAGGGTGAGGAGTGGAACAAGGCGAAGCGTGTA
GCTAAGGAGTTAGATCCCAGGTATGAAGACTATGTGGACCAGCATTATAAAGAGTTCCTCAAGAATCAG
GGCAAAGTGGACTCGCTGGTGGGTGTGGATGTGATAGCTGCTTTGGACCTGTATGTGGAGCAGGGCCAG
TGGGACAAGTGCATTGAAACAGCTACCAAGCAGAACTACAAGATTCTGCACAAGTATGTGGCTTTGTAT
GCAACTCACTTGATCCGGGAGGGTAGCTCTGCCCAGGCATTGGCCCTGTATGTACAGCACGGAGCCCCT
GCTAACCCACAGAACTTCAATATCTACAAAAGGATCTTCACTGACATGGTGAGCTCTCCTGGAACCAAC
TGTGCCGAGGCCTATCATAGCTGGGCTGATCTTCGAGATGTCCTCTTCAACCTGTGTGAAAACCTGGTG
AAGTCCAGTGAGGCAAACTCTCCAGCCCATGAGGAGTTCAAGACGATGCTGCTGATCGCTCATTACTAT
GCCACGCGCTCTGCAGCCCAGAGTGTCAAACAGCTGGAAACCGTGGCTGCCAGGCTTTCTGTTTCACTC
TTGCGTCACACCCAGCTACTACCTGTAGACAAAGCCTTCTATGAAGCAGGCATTGCTGCCAAGGCAGTT
GGCTGGGATAACATGGCATTCATCTTCCTCAATCGCTTTTTGGACCTGACCGATGCAATCGAGGAAGGG
ACTCTAGATGGCCTTGACCACTCTGATTTTCAGGATACAGACATTCCCTTTGAGGTGCCACTCCCAGCT
AAGCAGCATGTACCGGAGGCTGAGAGAGAAGAGGTTCGAGACTGGGTGCTTACAGTCTCCATGGACCAG
CGGCTGGAGCAGGTTCTGCCTCGGGATGAGCGTGGCGCCTACGAGGCCTCCCTAGTGGCAGCGAGCACT
GGTGTTCGAGCCCTGCCCTGCCTTATTACAGGATACCCCATTCTGAGGAACAAAATTGAATTTAAGCGG
CCAGGGAAGGCTGCTAACAAGGACAACTGGAATAAATTCCTTATGGCCATCAAGACCTCCCACAGCCCA
GTGTGCCAGGACGTGCTGAAATTCATCAGTCAGTGGTGTGGAGGGCTCCCCAGCACCAGCTTTTCCTTT
CAGTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_015662
Insert Size 5250 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015662.2
RefSeq Size 5488 bp
RefSeq ORF 5250 bp
Locus ID 26160
UniProt ID Q9UG01
Cytogenetics 2p23.3
MW 197.6 kDa
Summary This gene encodes a subunit of the intraflagellar transport subcomplex IFT-B. Subcomplexes IFT-A and IFT-B are necessary for ciliary assembly and maintenance. Mutations in this gene have been associated with skeletal ciliopathies, with or without polydactyly, such as such short-rib thoracic dysplasias 1, 9 or 10. provided by RefSeq, Mar 2014
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"NM_015662" in other vectors (2)
SKU Description Size Price
RC218799 IFT172 (Myc-DDK-tagged)-Human intraflagellar transport 172 homolog (Chlamydomonas) (IFT172) 10 ug
$1,470.00
RG218799 IFT172 (tGFP-tagged) - Human intraflagellar transport 172 homolog (Chlamydomonas) (IFT172) 10 ug
$1,670.00

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