CCBE1 (NM_133459) Human Untagged Clone

SKU
SC309783
CCBE1 (untagged)-Human collagen and calcium binding EGF domains 1 (CCBE1)
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CCBE1
Synonyms HKLLS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC309783 representing NM_133459.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGTGCCGCCGCCTCCGAGCCGGGGAGGAGCTGCCAGGGGCCAGCTGGGCAGGAGCCTGGGTCCGCTG
CTGCTGCTCCTGGCGTTGGGACACACGTGGACCTACAGAGAGGAGCCGGAGGACGGCGACAGAGAAATC
TGCTCAGAGAGCAAAATCGCGACGACTAAATACCCGTGTCTGAAGTCTTCAGGCGAGCTCACCACATGC
TACAGGAAAAAGTGCTGCAAAGGATATAAATTTGTTCTTGGACAATGCATCCCAGAAGATTACGACGTT
TGTGCCGAGGCTCCCTGTGAACAGCAGTGCACGGACAACTTTGGCCGAGTGCTGTGTACTTGTTATCCG
GGATACCGATATGACCGGGAGAGACACCGGAAGCGGGAGAAGCCATACTGTCTGGATATTGATGAGTGT
GCCAGCAGCAATGGGACGCTGTGTGCCCACATCTGCATCAATACCTTGGGCAGCTACCGCTGCGAGTGC
CGGGAAGGCTACATCCGGGAAGATGATGGGAAGACATGTACCAGGGGAGACAAATATCCCAATGACACT
GGCCATGAGAAGTCTGAGAACATGGTGAAAGCCGGAACTTGCTGTGCCACATGCAAGGAGTTCTACCAG
ATGAAGCAGACCGTGCTGCAGCTGAAGCAAAAGATTGCTCTGCTCCCCAACAATGCAGCTGACCTGGGC
AAGTATATCACTGGTGACAAGGTGCTGGCCTCAAACACCTACCTTCCAGGACCTCCTGGCCTGCCTGGG
GGCCAGGGCCCTCCCGGCTCACCAGGACCAAAGGGAAGCCCAGGCTTCCCCGGTATGCCAGGCCCTCCT
GGGCAGCCCGGCCCACGGGGCTCAATGGGACCCATGGGACCATCTCCTGATCTGTCCCACATTAAGCAA
GGCCGGAGGGGCCCTGTGGGTCCACCAGGGGCACCAGGAAGAGATGGTTCTAAGGGGGAGAGAGGAGCG
CCTGGGCCCAGAGGGTCTCCAGGACCCCCTGGTTCTTTCGACTTCCTGCTACTTATGCTGGCTGACATC
CGCAATGACATCACTGAGCTGCAGGAAAAGGTGTTCGGGCACCGGACTCACTCTTCAGCAGAGGAGTTC
CCTTTACCTCAGGAATTTCCCAGCTACCCAGAAGCCATGGACCTGGGCTCTGGAGATGACCATCCAAGA
AGAACTGAGACAAGAGACTTGAGAGCCCCCAGAGACTTCTACCCATAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_133459
Insert Size 1221 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133459.3
RefSeq Size 6276 bp
RefSeq ORF 1221 bp
Locus ID 147372
UniProt ID Q6UXH8
Cytogenetics 18q21.32
MW 44.1 kDa
Summary This gene is thought to function in extracellular matrix remodeling and migration. It is predominantly expressed in the ovary, but down regulated in ovarian cancer cell lines and primary carcinomas, suggesting its role as a tumour suppressor. Mutations in this gene have been associated with Hennekam lymphangiectasia-lymphedema syndrome, a generalized lymphatic dysplasia in humans. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_133459" in other vectors (6)
SKU Description Size Price
RC224203 CCBE1 (Myc-DDK-tagged)-Human collagen and calcium binding EGF domains 1 (CCBE1) 10 ug
$457.00
RC224203L1 Lenti ORF clone of Human collagen and calcium binding EGF domains 1 (CCBE1), Myc-DDK-tagged 10 ug
$757.00
RC224203L2 Lenti ORF clone of Human collagen and calcium binding EGF domains 1 (CCBE1), mGFP tagged 10 ug
$757.00
RC224203L3 Lenti ORF clone of Human collagen and calcium binding EGF domains 1 (CCBE1), Myc-DDK-tagged 10 ug
$757.00
RC224203L4 Lenti ORF clone of Human collagen and calcium binding EGF domains 1 (CCBE1), mGFP tagged 10 ug
$757.00
RG224203 CCBE1 (tGFP-tagged) - Human collagen and calcium binding EGF domains 1 (CCBE1) 10 ug
$657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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