NLRP14 (NM_176822) Human Untagged Clone

SKU SC309243

NLRP14 (untagged)-Human NLR family, pyrin domain containing 14 (NLRP14)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NLRP14
• Synonyms: CLR11.2; GC-LRR; NALP14; NOD5; PAN8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309243 representing NM_176822.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCAGATTCATCATCATCTTCTTTCTTTCCTGATTTTGGGCTGCTATTGTATTTGGAGGAGCTAAAC; AAAGAGGAATTAAATACATTCAAGTTATTCCTAAAGGAGACCATGGAACCTGAGCATGGCCTGACACCC; TGGAATGAAGTGAAGAAGGCCAGGCGGGAGGACCTGGCCAATTTGATGAAGAAATATTATCCAGGAGAG; AAAGCCTGGAGTGTGTCTCTCAAAATCTTTGGCAAGATGAACCTGAAGGATCTGTGTGAGAGAGCGAAA; GAAGAGATCAACTGGTCGGCCCAGACTATAGGACCAGATGATGCCAAGGCTGGAGAGACACAAGAAGAT; CAGGAGGCAGTGCTGGGTGATGGAACAGAATACAGAAATAGAATAAAGGAAAAATTTTGCATCACTTGG; GACAAGAAGTCTTTGGCTGGAAAGCCTGAAGATTTCCATCATGGAATTGCAGAGAAAGATAGAAAACTG; TTGGAACACTTGTTCGATGTGGATGTCAAAACCGGTGCACAGCCACAGATCGTGGTGCTTCAGGGAGCT; GCTGGAGTTGGGAAAACAACCTTGGTGAGAAAGGCAATGTTAGATTGGGCAGAGGGCAGTCTCTACCAG; CAGAGGTTTAAGTATGTTTTTTATCTCAATGGGAGAGAAATTAACCAGCTGAAAGAGAGAAGCTTTGCT; CAATTGATATCAAAGGACTGGCCCAGCACAGAAGGCCCCATTGAAGAAATCATGTACCAGCCAAGTAGC; CTCTTGTTTATTATTGACAGTTTCGATGAACTGAACTTTGCCTTTGAAGAACCTGAGTTTGCACTGTGC; GAAGACTGGACCCAAGAACACCCAGTGTCCTTCCTCATGAGTAGTTTGCTGAGGAAAGTGATGCTCCCT; GAGGCATCCTTATTGGTGACAACAAGACTCACAACTTCTAAGAGACTAAAGCAGTTGTTGAAGAATCAC; CATTATGTAGAGCTACTAGGAATGTCTGAGGATGCAAGAGAGGAGTATATTTACCAGTTTTTTGAAGAT; AAGAGGTGGGCCATGAAAGTATTCAGTTCACTAAAAAGCAATGAGATGCTGTTTAGCATGTGCCAAGTC; CCCCTAGTGTGCTGGGCCGCTTGTACTTGTCTGAAGCAGCAAATGGAGAAGGGTGGTGATGTCACATTG; ACCTGCCAAACAACCACAGCTCTGTTTACCTGCTATATTTCTAGCTTGTTCACACCAGTAGATGGAGGC; TCTCCTAGTCTACCCAACCAAGCCCAGCTGAGAAGACTGTGCCAAGTCGCTGCCAAAGGAATATGGACT; ATGACTTACGTGTTTTACAGAGAAAATCTCAGAAGGCTTGGGTTAACTCAATCTGATGTCTCTAGTTTT; ATGGACAGCAATATTATTCAGAAGGACGCAGAGTATGAAAACTGCTATGTGTTCACCCACCTTCATGTT; CAGGAGTTTTTTGCAGCTATGTTCTATATGTTGAAAGGCAGTTGGGAAGCTGGGAACCCTTCCTGCCAG; CCTTTTGAAGATTTGAAGTCATTACTTCAAAGCACAAGTTATAAAGACCCCCATTTGACACAGATGAAG; TGCTTTTTGTTTGGCCTTTTGAATGAAGATCGAGTAAAACAACTGGAGAGGACTTTTAACTGTAAAATG; TCACTGAAGATAAAATCAAAGTTACTTCAGTGTATGGAAGTATTAGGAAACAGTGACTATTCTCCATCA; CAGCTGGGATTTCTGGAGTTGTTTCACTGTCTGTATGAGACTCAAGATAAAGCGTTTATAAGCCAGGCA; ATGAGATGTTTCCCAAAGGTTGCCATTAATATTTGTGAGAAAATACATTTGCTTGTATCTTCTTTCTGC; CTTAAGCACTGCCGGTGTTTGCGGACCATCAGGCTGTCTGTAACTGTGGTATTTGAGAAGAAGATATTA; AAAACAAGCCTCCCAACTAACACTTGGGATGGTGATCGCATTACTCACTGTTGGCAAGATCTCTGTTCT; GTGCTTCATACAAATGAACACTTGAGAGAATTGGACCTGTACCATAGCAACCTTGATAAATCAGCAATG; AATATCCTGCATCATGAACTAAGGCACCCAAACTGTAAACTACAAAAGCTACTGTTGAAATTTATCACT; TTCCCTGATGGTTGTCAGGATATCTCTACTTCTTTGATTCATAACAAGAATCTGATGCATCTTGACCTA; AAAGGGAGTGATATAGGGGATAATGGAGTAAAGTCATTGTGTGAGGCCTTGAAACACCCAGAGTGTAAA; CTACAGACTCTCAGGCTGGAATCTTGCAACCTAACTGTATTTTGTTGTCTAAATATATCTAATGCTCTC; ATCAGAAGCCAGAGCCTGATATTTCTGAATCTGTCAACCAATAATCTGTTGGATGATGGAGTGCAGCTT; TTGTGTGAGGCCTTAAGACATCCAAAGTGTTATCTAGAGAGACTGTCCTTAGAAAGCTGTGGTCTCACA; GAGGCTGGCTGTGAGTATCTTTCTTTGGCTCTCATCAGCAATAAAAGACTGACACATTTGTGCTTGGCA; GACAATGTCTTGGGTGATGGTGGAGTAAAGCTTATGAGTGATGCCCTGCAACATGCACAATGTACTCTG; AAGAGCCTTGTGCTGAGGCGTTGCCATTTCACTTCACTTAGCAGTGAATATCTGTCAACTTCTCTTCTA; CACAACAAGAGCCTGACGCATCTGGATCTAGGATCAAACTGGCTACAAGACAATGGAGTGAAGCTTCTG; TGTGATGTCTTTCGGCATCCAAGCTGTAATCTTCAGGACTTGGAATTGATGGGCTGTGTTCTCACTAAT; GCATGTTGTCTGGATCTGGCTTCTGTTATTTTGAATAACCCAAACCTGAGGAGCCTGGACCTTGGGAAC; AACGATTTGCAGGATGATGGAGTGAAAATTCTGTGTGATGCTTTGAGATATCCAAACTGTAACATTCAG; AGGCTCGGGTTGGAATACTGTGGTTTGACATCTCTCTGCTGTCAAGATCTCTCCTCTGCTCTTATCTGC; AACAAAAGACTGATAAAAATGAATCTGACACAGAATACCTTAGGATATGAAGGAATTGTGAAGTTATAT; AAAGTCTTGAAGTCTCCTAAGTGTAAACTACAAGTTCTAGGGTTGTGCAAAGAGGCATTTGATGAGGAA; GCCCAGAAGCTGCTGGAAGCTGTGGGAGTTAGCAATCCACACTTAATCATTAAGCCAGATTGTAACTAT; CATAATGAAGAAGATGTGTCTTGGTGGTGGTGTTTCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_176822
• Insert Size: 3282 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_176822.3
• RefSeq Size: 3823 bp
• RefSeq ORF: 3282 bp
• Locus ID: 338323
• UniProt ID: Q86W24
• Cytogenetics: 11p15.4
• MW: 124.7 kDa
• Summary: The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). This protein may play a regulatory role in the innate immune system as similar family members belong to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. [provided by RefSeq, Jul 2008]