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EDC4 (NM_014329) Human Untagged Clone

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SKU
SC308921
EDC4 (untagged)-Human enhancer of mRNA decapping 4 (EDC4)
  $1,918.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EDC4
Synonyms Ge-1; GE1; HEDL5; HEDLS; RCD-8; RCD8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC308921 representing NM_014329.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCCTCCTGCGCGAGCATCGACATCGAGGACGCCACGCAGCACCTGCGGGACATCCTCAAGCTGGAC
CGGCCCGCGGGCGGCCCCAGTGCAGAGAGCCCACGGCCATCCAGTGCCTACAATGGGGACCTCAATGGA
CTTCTGGTCCCAGACCCGCTCTGCTCAGGTGATAGTACCTCAGCAAACAAGACTGGTCTTCGGACCATG
CCACCCATTAACCTGCAAGAGAAGCAGGTCATCTGTCTCTCAGGAGATGATAGCTCCACCTGCATTGGG
ATTTTGGCCAAGGAGGTGGAGATTGTGGCTAGCAGTGACTCTAGCATTTCAAGCAAGGCCCGGGGAAGC
AACAAGGTGAAAATTCAGCCTGTCGCCAAGTATGACTGGGAACAGAAGTACTACTATGGCAACCTGATT
GCTGTGTCTAACTCCTTCTTGGCCTATGCCATTCGGGCTGCCAACAATGGCTCTGCCATGGTGCGGGTG
ATCAGCGTCAGCACTTCGGAGCGGACCTTGCTCAAGGGCTTCACAGGCAGTGTGGCTGATCTGGCTTTC
GCGCACCTCAACTCTCCACAGCTGGCCTGCCTGGATGAGGCAGGCAACCTGTTCGTGTGGCGCTTGGCT
CTGGTTAATGGCAAAATTCAAGAAGAGATCTTGGTCCATATTCGGCAGCCAGAGGGCACGCCACTGAAC
CACTTTCGCAGGATCATCTGGTGCCCCTTCATCCCTGAGGAGAGCGAAGACTGCTGTGAGGAGAGCAGC
CCAACAGTGGCCCTGCTGCATGAAGACCGGGCTGAGGTGTGGGACCTGGACATGCTCCGCTCCAGCCAC
AGTACCTGGCCTGTGGATGTTAGCCAGATCAAGCAGGGCTTCATTGTGGTAAAAGGTCATAGCACGTGC
CTCAGTGAAGGAGCCCTCTCTCCTGATGGGACTGTGCTGGCTACTGCGAGCCACGATGGCTATGTCAAG
TTCTGGCAGATCTACATTGAGGGGCAAGATGAGCCAAGGTGTCTGCACGAGTGGAAACCTCATGATGGG
CGGCCCCTCTCCTGCCTCCTGTTCTGTGACAACCATAAGAAACAAGACCCTGATGTCCCTTTCTGGAGG
TTCCTTATTACTGGTGCTGACCAGAACCGAGAGTTAAAGATGTGGTGTACAGTATCCTGGACCTGCCTG
CAGACTATTCGCTTCTCCCCAGATATCTTCAGCTCAGTGAGTGTGCCCCCTAGCCTCAAGGTTTGCTTG
GACCTCTCAGCAGAATACCTGATTCTCAGCGATGTGCAACGGAAGGTCCTCTATGTGATGGAGCTGCTG
CAAAACCAGGAGGAGGGCCACGCCTGCTTCAGCTCCATCTCGGAGTTCCTGCTCACCCACCCTGTGCTG
AGCTTTGGTATCCAGGTTGTGAGTCGCTGCCGGCTACGGCACACTGAGGTGCTGCCTGCCGAAGAGGAA
AATGACAGCCTGGGTGCTGATGGTACCCATGGAGCCGGTGCCATGGAGTCTGCGGCCGGTGTGCTCATC
AAGCTCTTTTGTGTGCATACTAAGGCACTGCAAGATGTGCAGATCCGCTTCCAGCCACAGCTGAACCCT
GATGTGGTGGCCCCACTGCCCACCCACACTGCCCACGAGGACTTCACATTTGGAGAGTCTCGGCCCGAA
CTGGGCTCTGAGGGCCTGGGGTCAGCCGCTCACGGCTCCCAGCCTGACCTCCGACGAATCGTGGAGCTG
CCTGCACCTGCCGACTTCCTCAGTCTGAGCAGTGAGACCAAGCCCAAGTTGATGACACCTGACGCCTTC
ATGACACCTAGCGCCTCCTTGCAGCAGATCACTGCCTCTCCCAGCAGCAGCAGCAGCGGTAGCAGCAGC
AGCAGCAGCAGTAGCAGCAGCTCCCTTACAGCTGTGTCTGCCATGAGCAGCACCTCAGCTGTGGACCCC
TCCTTGACCAGGCCACCTGAGGAGCTGACCTTGAGCCCCAAGCTGCAGCTGGATGGCAGCCTGACAATG
AGCAGCAGTGGCAGCCTTCAGGCAAGCCCGCGTGGCCTCCTGCCTGGCCTGCTCCCAGCCCCAGCTGAC
AAACTGACTCCCAAGGGGCCGGGCCAGGTGCCTACTGCCACCTCTGCACTGTCCCTGGAGCTGCAGGAA
GTGGAGCCCCTGGGGCTACCCCAAGCCTCCCCTAGCCGCACTCGTTCCCCTGATGTCATCTCCTCAGCT
TCCACTGCCCTGTCCCAGGACATCCCTGAGATTGCATCTGAGGCCCTGTCCCGTGGTTTTGGCTCCTCT
GCACCAGAGGGCCTTGAGCCAGACAGTATGGCTTCAGCCGCCTCGGCACTGCACCTGCTGTCCCCACGG
CCCCGGCCAGGGCCCGAGCTCGGCCCCCAGCTCGGGCTTGATGGAGGCCCTGGGGATGGAGATCGGCAT
AATACCCCCTCCCTCCTGGAGGCAGCCTTGACCCAGGAGGCCTCGACTCCTGACAGTCAGGTTTGGCCC
ACAGCACCTGACATTACTCGTGAGACCTGCAGCACCCTGGCAGAAAGCCCCAGGAATGGCCTTCAGGAA
AAGCACAAGAGCCTGGCCTTCCACCGACCACCATATCACCTGCTGCAGCAACGTGACAGCCAGGATGCC
AGTGCTGAGCAAAGTGACCATGATGATGAGGTGGCCAGCCTTGCCTCTGCTTCAGGAGGCTTTGGCACC
AAAGTTCCTGCTCCACGGCTGCCTGCCAAGGACTGGAAGACCAAGGGATCCCCTCGAACCTCACCCAAG
CTCAAGAGGAAAAGCAAGAAGGATGATGGGGATGCAGCCATGGGATCCCGGCTCACAGAGCACCAGGTG
GCAGAGCCCCCTGAGGACTGGCCAGCACTAATTTGGCAACAGCAGAGAGAGCTGGCAGAGCTGCGGCAC
AGCCAGGAAGAGCTGCTGCAGCGTCTGTGTACCCAACTCGAAGGCCTGCAGAGCACAGTCACAGGCCAC
GTAGAACGTGCCCTTGAGACTCGGCACGAGCAGGAACAGCGGCGGCTGGAGCGAGCACTGGCTGAGGGG
CAGCAGCGGGGAGGGCAGCTGCAGGAGCAGCTGACACAACAGTTGTCCCAAGCACTGTCGTCAGCTGTA
GCTGGGCGGCTAGAGCGCAGCATACGGGATGAGATCAAGAAGACAGTCCCTCCATGTGTCTCAAGGAGT
CTGGAGCCTATGGCAGGCCAACTGAGCAACTCAGTGGCTACCAAGCTCACAGCTGTGGAGGGCAGCATG
AAAGAGAACATCTCCAAGCTGCTCAAGTCCAAGAACTTGACTGATGCCATCGCCCGAGCAGCTGCAGAC
ACATTACAAGGGCCGATGCAGGCTGCCTACCGGGAAGCCTTCCAGAGTGTGGTGCTGCCGGCCTTTGAG
AAGAGCTGCCAGGCCATGTTCCAGCAAATCAATGATAGCTTCCGGCTGGGGACACAGGAATACTTGCAG
CAGCTAGAAAGCCACATGAAGAGCCGGAAGGCACGGGAACAGGAGGCCAGGGAGCCTGTGCTAGCCCAG
CTGCGGGGCCTGGTCAGCACACTGCAGAGTGCCACTGAGCAGATGGCAGCCACCGTGGCCGGCAGTGTT
CGTGCTGAGGTGCAGCACCAGCTGCATGTGGCTGTGGGCAGCCTGCAGGAGTCCATTTTAGCACAGGTA
CAGCGCATCGTTAAGGGTGAGGTGAGTGTGGCGCTCAAGGAGCAGCAGGCCGCCGTCACCTCCAGCATC
ATGCAGGCCATGCGCTCAGCTGCTGGCACACCTGTCCCCTCTGCCCACCTTGACTGCCAGGCCCAGCAA
GCCCATATCCTGCAGCTGCTGCAGCAGGGCCACCTCAATCAGGCCTTCCAGCAGGCGCTGACAGCTGCT
GACCTGAACCTGGTGCTGTATGTGTGTGAAACTGTGGACCCAGCCCAGGTTTTTGGGCAGCCACCCTGC
CCGCTCTCCCAGCCTGTGCTCCTTTCCCTCATCCAGCAGCTGGCATCTGACCTTGGCACTCGAACTGAC
CTCAAGCTCAGCTACCTGGAAGAGGCCGTGATGCACCTGGACCACAGTGACCCCATCACTCGGGACCAC
ATGGGCTCCGTTATGGCCCAGGTGCGCCAAAAGCTTTTTCAGTTCCTGCAGGCTGAGCCACACAACTCA
CTTGGCAAAGCAGCTCGGCGTCTCAGCCTCATGCTGCATGGCCTCGTGACCCCCAGCCTCCCTTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_014329
Insert Size 4206 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014329.4
RefSeq Size 4811 bp
RefSeq ORF 4206 bp
Locus ID 23644
UniProt ID Q6P2E9
Cytogenetics 16q22.1
Domains WD40
Protein Pathways RNA degradation
MW 151.7 kDa
Summary In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_014329" in other vectors (6)
SKU Description Size Price
RC222770 EDC4 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 4 (EDC4) 10 ug
$1,797.00
RC222770L1 Lenti-ORF clone of EDC4 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 4 (EDC4) 10 ug
$2,097.00
RC222770L2 Lenti-ORF clone of EDC4 (mGFP-tagged)-Human enhancer of mRNA decapping 4 (EDC4) 10 ug
$2,097.00
RC222770L3 Lenti-ORF clone of EDC4 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 4 (EDC4) 10 ug
$2,097.00
RC222770L4 Lenti-ORF clone of EDC4 (mGFP-tagged)-Human enhancer of mRNA decapping 4 (EDC4) 10 ug
$2,097.00
RG222770 EDC4 (tGFP-tagged) - Human enhancer of mRNA decapping 4 (EDC4) 10 ug
$1,997.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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