BRDT (NM_207189) Human Untagged Clone

SKU
SC308409
BRDT (untagged)-Human bromodomain, testis-specific (BRDT), transcript variant 1
  $1,388.00
2 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol BRDT
Synonyms BRD6; CT9; SPGF21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_207189
Insert Size 2844 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207189.3
RefSeq Size 3361 bp
RefSeq ORF 2844 bp
Locus ID 676
UniProt ID Q58F21
Cytogenetics 1p22.1
Protein Families Protein Kinase, Transcription Factors
MW 108 kDa
Summary BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. provided by RefSeq, Jun 2011
Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 4. The resulting isoform (b) lacks a short internal segment compared to isoform a. Variants 1, 2, and 3 all encode the same isoform (b).
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"NM_207189" in other vectors (6)
SKU Description Size Price
RC222276 BRDT (Myc-DDK-tagged)-Human bromodomain, testis-specific (BRDT), transcript variant 1 10 ug
$1,300.00
RC222276L1 Lenti-ORF clone of BRDT (Myc-DDK-tagged)-Human bromodomain, testis-specific (BRDT), transcript variant 1 10 ug
$1,600.00
RC222276L2 Lenti-ORF clone of BRDT (mGFP-tagged)-Human bromodomain, testis-specific (BRDT), transcript variant 1 10 ug
$1,600.00
RC222276L3 Lenti-ORF clone of BRDT (Myc-DDK-tagged)-Human bromodomain, testis-specific (BRDT), transcript variant 1 10 ug
$1,600.00
RC222276L4 Lenti-ORF clone of BRDT (mGFP-tagged)-Human bromodomain, testis-specific (BRDT), transcript variant 1 10 ug
$1,600.00
RG222276 BRDT (tGFP-tagged) - Human bromodomain, testis-specific (BRDT), transcript variant 1 10 ug
$1,500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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