ACSL5 (NM_203380) Human Untagged Clone

SKU SC308146

ACSL5 (untagged)-Human acyl-CoA synthetase long-chain family member 5 (ACSL5), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: ACSL5
• Synonyms: ACS2; ACS5; FACL5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308146 representing NM_203380.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCTTTTTATCTTTAACTTTTTGTTTTCCCCACTTCCGACCCCGGCGTTGATCTGCATCCTGACATTT; GGAGCTGCCATCTTCTTGTGGCTGATCACCAGACCTCAACCCGTCTTACCTCTTCTTGACCTGAACAAT; CAGTCTGTGGGAATTGAGGGAGGAGCACGGAAGGGGGTTTCCCAGAAGAACAATGACCTAACAAGTTGC; TGCTTCTCAGATGCCAAGACTATGTATGAGGTTTTCCAAAGAGGACTCGCTGTGTCTGACAATGGGCCC; TGCTTGGGATATAGAAAACCAAACCAGCCCTACAGATGGCTATCTTACAAACAGGTGTCTGATAGAGCA; GAGTACCTGGGTTCCTGTCTCTTGCATAAAGGTTATAAATCATCACCAGACCAGTTTGTCGGCATCTTT; GCTCAGAATAGGCCAGAGTGGATCATCTCCGAATTGGCTTGTTACACGTACTCTATGGTAGCTGTACCT; CTGTATGACACCTTGGGACCAGAAGCCATCGTACATATTGTCAACAAGGCTGATATCGCCATGGTGATC; TGTGACACACCCCAAAAGGCATTGGTGCTGATAGGGAATGTAGAGAAAGGCTTCACCCCGAGCCTGAAG; GTGATCATCCTTATGGACCCCTTTGATGATGACCTGAAGCAAAGAGGGGAGAAGAGTGGAATTGAGATC; TTATCCCTATATGATGCTGAGAACCTAGGCAAAGAGCACTTCAGAAAACCTGTGCCTCCTAGCCCAGAA; GACCTGAGCGTCATCTGCTTCACCAGTGGGACCACAGGTGACCCCAAAGGAGCCATGATAACCCATCAA; AATATTGTTTCAAATGCTGCTGCCTTTCTCAAATGTGTGGAGCATGCTTATGAGCCCACTCCTGATGAT; GTGGCCATATCCTACCTCCCTCTGGCTCATATGTTTGAGAGGATTGTACAGGCTGTTGTGTACAGCTGT; GGAGCCAGAGTTGGATTCTTCCAAGGGGATATTCGGTTGCTGGCTGACGACATGAAGACTTTGAAGCCC; ACATTGTTTCCCGCGGTGCCTCGACTCCTTAACAGGATCTACGATAAGGTACAAAATGAGGCCAAGACA; CCCTTGAAGAAGTTCTTGTTGAAGCTGGCTGTTTCCAGTAAATTCAAAGAGCTTCAAAAGGGTATCATC; AGGCATGATAGTTTCTGGGACAAGCTCATCTTTGCAAAGATCCAGGACAGCCTGGGCGGAAGGGTTCGT; GTAATTGTCACTGGAGCTGCCCCCATGTCCACTTCAGTCATGACATTCTTCCGGGCAGCAATGGGATGT; CAGGTGTATGAAGCTTATGGTCAAACAGAATGCACAGGTGGCTGTACATTTACATTACCTGGGGACTGG; ACATCAGGTCACGTTGGGGTGCCCCTGGCTTGCAATTACGTGAAGCTGGAAGATGTGGCTGACATGAAC; TACTTTACAGTGAATAATGAAGGAGAGGTCTGCATCAAGGGTACAAACGTGTTCAAAGGATACCTGAAG; GACCCTGAGAAGACACAGGAAGCCCTGGACAGTGATGGCTGGCTTCACACAGGAGACATTGGTCGCTGG; CTCCCGAATGGAACTCTGAAGATCATCGACCGTAAAAAGAACATTTTCAAGCTGGCCCAAGGAGAATAC; ATTGCACCAGAGAAGATAGAAAATATCTACAACAGGAGTCAACCAGTGTTACAAATTTTTGTACACGGG; GAGAGCTTACGGTCATCCTTAGTAGGAGTGGTGGTTCCTGACACAGATGTACTTCCCTCATTTGCAGCC; AAGCTTGGGGTGAAGGGCTCCTTTGAGGAACTGTGCCAAAACCAAGTTGTAAGGGAAGCCATTTTAGAA; GACTTGCAGAAAATTGGGAAAGAAAGTGGCCTTAAAACTTTTGAACAGGTCAAAGCCATTTTTCTTCAT; CCAGAGCCATTTTCCATTGAAAATGGGCTCTTGACACCAACATTGAAAGCAAAGCGAGGAGAGCTTTCC; AAATACTTTCGGACCCAAATTGACAGCCTGTATGAGCACATCCAGGATTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_203380
• Insert Size: 2052 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_203380.1
• RefSeq Size: 3399 bp
• RefSeq ORF: 2052 bp
• Locus ID: 51703
• UniProt ID: Q9ULC5
• Cytogenetics: 10q25.2
• Protein Families: Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• MW: 76 kDa
• Summary: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (3) differs in the 5' UTR and coding region compared to variant 1. This results in translation initiation from a downstream ATG and an isoform (b) that has a shorter N-terminus compared to isoform a. Both variants 2 and 3 encode isoform b.