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RAPH1 (NM_203365) Human Untagged Clone

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SKU
SC308137
RAPH1 (untagged)-Human Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 (RAPH1), transcript variant 3
  $960.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RAPH1
Synonyms ALS2CR9; ALS2CR18; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC308137 representing NM_203365.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGCAGCTATCAGATGAAGAAATTGATCATGGTGCTGAAGAAGACAGTGACAAGGAAGATCAGGAC
CTGGACAAAATGTTTGGAGCCTGGCTTGGAGAACTAGACAAACTCACTCAGAGTTTGGATTCTGACAAG
CCCATGGAACCAGTAAAAAGATCTCCTCTTCGCCAGGAAACAAACATGGCCAACTTTTCTTACCGCTTC
TCCATATACAACTTGAATGAAGCTCTGAATCAGGGAGAGACTGTGGATCTGGATGCCTTGATGGCTGAT
CTTTGCTCTATAGAGCAGGAGCTCAGCAGCATTGGTTCAGGAAACAGTAAGCGTCAAATCACAGAAACG
AAAGCTACTCAGAAATTGCCTGTTAGCCGACATACATTGAAACATGGCACCTTGAAAGGATTATCTTCT
TCATCTAATAGGATAGCTAAACCTTCCCATGCCAGCTACTCCTTGGACGACGTCACTGCACAGTTAGAA
CAGGCCTCTTTGAGTATGGATGAGGCTGCTCAGCAATCTGTACTAGAAGATACTAAACCCTTAGTAACT
AATCAGCACAGAAGAACCGCGTCAGCAGGCACAGTGAGTGATGCTGAAGTACACTCTATTAGTAATTCC
TCCCATTCCAGCATCACTTCCGCAGCCTCCAGCATGGACTCTTTGGATATTGATAAAGTAACACGCCCT
CAAGAGCTGGATTTGACACATCAAGGGCAGCCAATTACTGAGCATGCTATTTCTCTGAGATGTTCCAGC
AAGCAGGCCAAGCGTCATATTGACTTCACAGAAGAACAGGCTGAGCTGACACCTCACTCCTATCTGGAC
AGGGAAACCTCCCTTCTTCTGAGAAACATTGCAGGAAAACCTTCTCATTTGCTGACCAAGGAAGAACAG
GCAGCAAAATTGAAAGCTGAGAAGATCAGAGTTGCCCTAGAGAAAATTAAAGAGGCACAAGTGAAAAAG
CTGGTGATCAGAGTCCACATGTCTGATGACAGTTCTAAAACAATGATGGTGGATGAGAGGCAGACAGTA
AGACAAGTACTGGATAACCTGATGGACAAATCCCACTGCGGTTATAGTTTAGACTGGTCACTGGTAGAA
ACCGTTTCTGAATTACAAATGGAGAGAATCTTTGAAGACCATGAAAACTTGGTTGAAAATCTTCTTAAT
TGGACAAGAGATAGCCAAAACAAGCTTATATTTATGGAGCGTATAGAAAAATATGCACTTTTCAAAAAC
CCACAGAATTATCTTTTGGGGAAAAAAGAAACAGCTGAGATGGCAGATAGAAACAAAGAAGTCCTCTTG
GAGGAATGTTTTTGTGGAAGTTCTGTAACTGTACCAGAAATTGAAGGAGTCCTTTGGTTGAAGGATGAT
GGCAAGAAGTCCTGGAAAAAGCGTTATTTTCTCTTGCGAGCATCTGGTATCTACTATGTTCCCAAAGGA
AAAGCAAAGGTCTCTCGGGATCTGGTGTGCTTTCTCCAGCTGGATCATGTCAACGTTTATTATGGCCAG
GACTATCGGAACAAATACAAAGCACCTACAGACTATTGTCTGGTGCTGAAGCATCCACAAATCCAGAAG
AAATCTCAATATATCAAATACCTTTGTTGTGATGATGTGAGGACACTGCATCAGTGGGTCAATGGGATC
CGCATTGCAAAGTATGGGAAGCAGCTCTATATGAACTACCAAGAAGCCTTGAAGAGGACAGAGTCAGCC
TATGATTGGACTTCCTTATCCAGCTCCAGCATTAAATCGGGATCCAGTTCTTCCAGCATCCCAGAGTCT
CAGTCAAACCACTCCAATCAGTCTGATAGCGGAGTTTCTGACACCCAGCCAGCAGGACACGTCCGTTCC
CAGAGCATTGTGAGCTCCGTATTCTCTGAAGCCTGGAAACGAGGCACTCAGTTGGAAGAGTCCAGCAAG
TAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_203365
Insert Size 1935 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_203365.3
RefSeq Size 2782 bp
RefSeq ORF 1935 bp
Locus ID 65059
UniProt ID Q70E73
Cytogenetics 2q33.2
MW 72.9 kDa
Summary This gene encodes a protein that belongs to the Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of adapter proteins, which function in cell migration. Members of this family contain pleckstrin-homology domains, Ras-association domains, and proline-rich C-termini. The protein encoded by this gene regulates actin dynamics through interaction with Ena/Vasodilator proteins as well as direct binding to filamentous actin to regulate actin network assembly. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2016
Transcript Variant: This variant (3) has two consecutive additional exons in the coding region and an alternate 3' terminal exon, which results in an immediate translation termination, compared to variant 1. The resulting isoform (3) has an additional int
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Documents
Resources
"NM_203365" in other vectors (4)
SKU Description Size Price
RC216153 RAPH1 (Myc-DDK-tagged)-Human Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 (RAPH1), transcript variant 3 10 ug
$899.00
RC216153L3 Lenti ORF clone of Human Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 (RAPH1), transcript variant 3, Myc-DDK-tagged 10 ug
$1,199.00
RC216153L4 Lenti ORF clone of Human Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 (RAPH1), transcript variant 3, mGFP tagged 10 ug
$1,199.00
RG216153 RAPH1 (tGFP-tagged) - Human Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 (RAPH1), transcript variant 3 10 ug
$1,099.00

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