FAM86A (EEF2KMT) (NM_201598) Human Untagged Clone

SKU
SC308071
FAM86A (untagged)-Human family with sequence similarity 86, member A (FAM86A), transcript variant 2
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol FAM86A
Synonyms eEF2-KMT; EFM3; FAM86A; SB153
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC308071 representing NM_201598.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGCCCGAGGAGAACGCGGGGACCGAACTCTTGCTGCAGAGTTTCGAGCGCCGCTTCCTGGCGGCA
CGCACACTGCGCTCCTTCCCCTGGCAGAGCTTAGAAGCAAAGTTAAGAGACTCATCAGATTCTGAGCTG
CTGCGGGATATTTTGCACAAGACTGTGAAGCATCCTGTGTGTGTGAAGCACCCGCCGTCCGTCAAATAT
GCCCGGTGCTTTCTCTCAGAACTCATCAAAAAGCCCTCGGGAGGCTCGGTCACACTCTCCGAGAGCACG
GCCATCATCTCCTACGGTACCACAGGCCTGGTCACATGGGACGCCGCCCTCTACCTTGCAGAATGGGCC
ATCGAGAACCCGGCAGTCTTCACTAACAGGACTGTCCTAGAGCTTGGCAGTGGTGCTGGCCTCACAGGC
CTGGCCATCTGCAAGATGTGCCGCCCCCGGGCATACATCTTCAGCGACTGTCACAGCCGGGTCCTTGAG
CAGCTCCGAGGGAATGTCCTTCTCAATGGCCTCTCATTAGAGGCAGACATCACTGCCAAGTTAGACAGC
CCCAGGGTGACAGTGGCCCAGCTGGACTGGGACGTCGCGACGGTCCATCAGCTCTCTGCCTTCCAGCCA
GATGTTGTCATTGCAGCAGATGTGCTGTATTGCCCAGAAGCCATCATGTCGCTGGTCGGCGTCCTGCGG
AGGCTGGCTGCCTGCCGGGAGCACCAGCGGGCTCCTGAGGTCTACGTGGCCTTTACCGTCCGCAACCCA
GAGACGTGCCAGCTGTTCACCACCGAGCTAGGCCGGGCCGGGATCAGATGGGAAGTGGAACCTCGTCAT
GAGCAGAAACTGTTTCCCTACGAAGAGCACTTGGAGATGGCAATGCTGAATCTCACCCTGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_201598
Insert Size 891 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201598.3
RefSeq Size 2309 bp
RefSeq ORF 891 bp
Locus ID 196483
UniProt ID Q96G04
Cytogenetics 16p13.3
MW 33.1 kDa
Summary Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.
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Documents
Resources
"NM_201598" in other vectors (4)
SKU Description Size Price
RC212357 FAM86A (Myc-DDK-tagged)-Human family with sequence similarity 86, member A (FAM86A), transcript variant 2 10 ug
$300.00
RC212357L3 Lenti ORF clone of Human family with sequence similarity 86, member A (FAM86A), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC212357L4 Lenti ORF clone of Human family with sequence similarity 86, member A (FAM86A), transcript variant 2, mGFP tagged 10 ug
$600.00
RG212357 FAM86A (tGFP-tagged) - Human family with sequence similarity 86, member A (FAM86A), transcript variant 2 10 ug
$500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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