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CERKL (NM_201548) Human Untagged Clone

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SKU
SC308051
CERKL (untagged)-Human ceramide kinase-like (CERKL), transcript variant 1
  $546.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CERKL
Synonyms RP26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC308051 representing NM_201548.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCCTGGAGGAGGCGCAGGAACCGGGTGAGTGCCCTGGAGGGCGGCCGGGAGGAAGAGGCGCCCCCG
GAGGCTGCCGCTGTGCCTCCGGCGCTGTTAACGTCCCCGCAGCAGACGGAGGCGGCGGCCGAGCGGATT
CTGCTCCGGGGCATCTTCGAGATCGGGAGGGACAGTTGTGACGTGGTGCTGAGCGAGCGAGCACTGCGG
TGGCGGCCCATTCAGCCCGAGCGCCCGGCGGGTGATTCTAAGTATGACTTGCTATGTAAAGAAGAATTT
ATTGAACTCAAAGACATATTCTCTGTGAAACTGAAACGGCGTTGTTCTGTTAAACAGCAGAGAAGTGGT
ACTTTATTAGGTATCACACTCTTCATCTGCTTGAAAAAGGAACAAAATAAACTAAAGAATTCTACACTT
GATCTTATTAATTTAAGTGAAGACCACTGTGACATATGGTTTAGACAGTTCAAGAAAATATTGGCAGGC
TTTCCAAACAGACCGAAGTCATTAAAAATACTCCTTAACCCCCAAAGTCACAAAAAAGAAGCTACCCAG
GTTTATTATGAGAAGGTTGAACCTCTGTTGAAGCTTGCAGGAATAAAAACTGATGTAACAATAATGGAA
TATGAAGGGCACGCTCTGTCACTGCTTAAGGAATGTGAACTCCAGGGATTTGATGGTGTTGTCTGTGTT
GGTGGAGATGGATCTGCTAGCGAAGTAGCCCATGCTTTGCTTCTGAGAGCTCAGAAGAATGCTGGGATG
GAAACAGACCGAATCCTGACTCCTGTCAGAGCACAGCTTCCACTTGGCTTAATACCAGCAGGATCTACC
AATGTATTGGCACATTCTCTTCATGGAGTTCCTCATGTGATAACTGCAACATTGCACATTATAATGGGG
CATGTACAGCTGGTCGACGTCTGCACCTTCAGCACCGCTGGCAAGCTTCTTCGCTTTGGGTTCTCAGCC
ATGTTTGGCTTTGGTGGAAGAACTTTGGCTCTGGCAGAAAAATATCGATGGATGTCCCCTAACCAACGG
AGAGATTTTGCTGTTGTTAAGGCACTGGCAAAACTTAAGGCAGAAGACTGTGAAATATCATTTTTACCA
TTTAACAGCTCTGATGATGTGCAAGAAAGGAGGGCACAGGGATCTCCCAAATCTGACTGTAATGATCAA
TGGCAAATGATCCAGGGTCAGTTCTTGAATGTCAGCATTATGGCAATTCCTTGCCTGTGTTCAGTGGCA
CCTAGAGGCTTGGCACCTAATACCAGATTAAATAATGGAAGTATGGCTCTTATAATTGCCCGAAACACT
TCTCGGCCAGAATTTATAAAACACCTGAAAAGATATGCCAGTGTAAAAAATCAGTTCAATTTTCCATTT
GTTGAGACTTACACTGTTGAGGAAGTAAAAGTTCATCCAAGGAATAATACTGGTGGATATAATCCAGAG
GAGGAGGAGGATGAAACTGCTTCAGAAAATTGTTTCCCTTGGAATGTAGATGGTGACTTAATGGAAGTT
GCATCAGAGGTCCATATTAGATTGCATCCAAGACTTATCAGTCTTTATGGAGGAAGCATGGAAGAAATG
ATTCCAAAGTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_201548
Insert Size 1599 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201548.4
RefSeq Size 3226 bp
RefSeq ORF 1599 bp
Locus ID 375298
UniProt ID Q49MI3
Cytogenetics 2q31.3
Protein Families Druggable Genome
MW 59.6 kDa
Summary This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.provided by RefSeq, May 2010
Transcript Variant: This variant (1) lacks one alternate in-frame exon in the central coding region, compared to variant 2. The resulting isoform (1) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_201548" in other vectors (6)
SKU Description Size Price
RC223592 CERKL (Myc-DDK-tagged)-Human ceramide kinase-like (CERKL), transcript variant 1 10 ug
$495.00
RC223592L1 Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 1, Myc-DDK-tagged 10 ug
$795.00
RC223592L2 Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 1, mGFP tagged 10 ug
$795.00
RC223592L3 Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 1, Myc-DDK-tagged 10 ug
$795.00
RC223592L4 Lenti ORF clone of Human ceramide kinase-like (CERKL), transcript variant 1, mGFP tagged 10 ug
$795.00
RG223592 CERKL (tGFP-tagged) - Human ceramide kinase-like (CERKL), transcript variant 1 10 ug
$695.00

file-alt Citations

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