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MPRIP (NM_201274) Human Untagged Clone

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SKU
SC308006
MPRIP (untagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2
  $982.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MPRIP
Synonyms M-RIP; MRIP; p116Rip; RHOIP3; RIP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC308006 representing NM_201274.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCGGCAGCCAAGGAGAACCCGTGCAGGAAATTCCAGGCCAACATCTTCAACAAGAGCAAGTGTCAG
AACTGCTTCAAGCCCCGCGAGTCGCATCTGCTCAACGACGAGGACCTGACGCAGGCAAAACCCATTTAT
GGCGGTTGGCTGCTCCTGGCTCCAGATGGGACCGACTTTGACAACCCAGTGCACCGGTCTCGGAAATGG
CAGCGACGGTTCTTCATCCTTTACGAGCACGGCCTCTTGCGCTACGCCCTGGATGAGATGCCCACGACC
CTTCCTCAGGGCACCATCAACATGAACCAGTGCACAGATGTGGTGGATGGGGAGGGCCGCACGGGCCAG
AAGTTCTCCCTGTGTATTCTGACGCCTGAGAAGGAGCATTTCATCCGGGCGGAGACCAAGGAGATCGTC
AGTGGGTGGCTGGAGATGCTCATGGTCTATCCCCGGACCAACAAGCAGAATCAGAAGAAGAAACGGAAA
GTGGAGCCCCCCACACCACAGGAGCCTGGGCCTGCCAAGGTGGCTGTTACCAGCAGCAGCAGCAGCAGC
AGCAGCAGCAGCAGCATCCCCAGTGCTGAGAAAGTCCCCACCACCAAGTCCACACTCTGGCAGGAAGAA
ATGAGGACCAAGGACCAGCCAGATGGCAGCAGCCTGAGTCCAGCTCAGAGTCCCAGCCAGAGCCAGCCT
CCTGCTGCCAGCTCCCTGCGGGAACCTGGGCTAGAGAGCAAAGAAGAGGAGAGCGCCATGAGTAGCGAC
CGCATGGACTGTGGCCGCAAAGTCCGGGTGGAGAGCGGCTACTTCTCTCTGGAGAAGACCAAACAGGAC
TTGAAGGCTGAAGAACAGCAGCTGCCCCCGCCGCTCTCCCCTCCCAGCCCCAGCACCCCCAACCACAGG
AGGTCCCAGGTGATTGAAAAGTTTGAGGCCTTGGACATTGAGAAGGCAGAGCACATGGAGACCAATGCA
GTGGGGCCCTCACCATCCAGCGACACACGCCAGGGCCGCAGCGAGAAGAGGGCGTTCCCTAGGAAGCGG
GACTTCACCAATGAAGCCCCCCCAGCTCCTCTCCCAGACGCCTCGGCTTCCCCCCTGTCTCCACACCGA
AGAGCCAAGTCACTGGACAGGAGGTCCACGGAGCCCTCCGTGACGCCCGACCTGCTGAATTTCAAGAAA
GGCTGGCTGACTAAGCAGTATGAGGACGGCCAGTGGAAGAAACACTGGTTTGTCCTCGCCGATCAAAGC
CTGAGATACTACAGGGATTCAGTGGCTGAGGAGGCAGCCGACTTGGATGGAGAAATTGACTTGTCCGCA
TGTTACGATGTCACAGAGTATCCAGTTCAGAGAAACTATGGCTTCCAGATACATACAAAGGAGGGCGAG
TTTACCCTGTCGGCCATGACATCTGGGATTCGGCGGAACTGGATCCAGACCATCATGAAGCACGTGCAC
CCGACCACTGCCCCGGATGTGACCAGCTCGTTGCCAGAGGAAAAAAACAAGAGCAGCTGCTCTTTTGAG
ACCTGCCCGAGGCCTACTGAGAAGCAAGAGGCAGAGCTGGGGGAGCCGGACCCTGAGCAGAAGAGGAGC
CGCGCACGGGAGCGGAGGCGAGAGGGCCGCTCCAAGACCTTTGACTGGGCTGAGTTCCGTCCCATCCAG
CAGGCCCTGGCTCAGGAGCGGGTGGGCGGCGTGGGGCCTGCTGACACCCACGAGCCCCTGCGCCCTGAG
GCGGAGCCTGGGGAGCTGGAGCGGGAGCGTGCACGGAGGCGGGAGGAGCGCCGCAAGCGCTTCGGGATG
CTCGACGCCACAGACGGGCCAGGCACTGAGGATGCAGCCCTGCGCATGGAGGTGGACCGGAGCCCAGGG
CTGCCTATGAGCGACCTCAAAACGCATAACGTCCACGTGGAGATTGAGCAGCGGTGGCATCAGGTGGAG
ACCACACCTCTCCGGGAAGAGAAGCAGGTGCCCATCGCCCCCGTCCACCTGTCTTCTGAAGATGGGGGT
GACCGGCTCTCCACACACGAGCTGACCTCTCTGCTCGAGAAGGAGCTGGAGCAGAGCCAGAAGGAGGCC
TCAGACCTTCTGGAGCAGAACCGGCTCCTGCAGGACCAGCTGAGGGTGGCCCTGGGCCGGGAGCAGAGC
GCCCGTGAGGGCTACGTGCTGCAGGCCACGTGCGAGCGAGGGTTTGCAGCAATGGAAGAAACGCACCAG
AAGAAGATTGAAGATCTCCAGAGGCAGCACCAGCGGGAGCTAGAGAAACTTCGAGAAGAGAAAGACCGC
CTCCTAGCCGAGGAGACAGCGGCCACCATCTCAGCCATCGAAGCCATGAAGAACGCCCACCGGGAGGAA
ATGGAGCGGGAGCTGGAGAAGAGCCAGCGGTCCCAGATCAGCAGCGTCAACTCGGATGTTGAGGCCCTG
CGGCGCCAGTACCTGGAGGAGCTGCAGTCGGTGCAGCGGGAACTGGAGGTCCTCTCGGAGCAGTACTCG
CAGAAGTGCCTGGAGAATGCCCATCTGGCCCAGGCGCTGGAGGCCGAGCGGCAGGCCCTGCGGCAGTGC
CAGCGTGAGAACCAGGAGCTCAATGCCCACAACCAGGAGCTGAACAACCGCCTGGCTGCAGAGATCACA
CGGTTGCGGACGCTGCTGACTGGGGACGGCGGTGGGGAGGCCACTGGGTCACCCCTTGCACAGGGCAAG
GATGCCTATGAACTAGAGGTCTTATTGCGGGTAAAGGAATCGGAAATACAGTACCTGAAACAGGAGATT
AGCTCCCTCAAGGATGAGCTGCAGACGGCACTGCGGGACAAGAAGTACGCAAGTGACAAGTACAAAGAC
ATCTACACAGAGCTCAGCATCGCGAAGGCTAAGGCTGACTGTGACATCAGCAGGTTGAAGGAGCAGCTC
AAGGCTGCAACGGAAGCACTGGGGGAGAAGTCCCCTGACAGTGCCACGGTGTCCGGATATGATATAATG
AAATCTAAAAGCAACCCTGACTTCTTGAAGAAAGACAGATCCTGTGTCACCCGGCAACTCAGAAACATC
AGGTCCAAGTCCGTAATTGAGCAGGTCTCGTGGGATACCTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_201274
Insert Size 3078 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201274.3
RefSeq Size 11023 bp
RefSeq ORF 3078 bp
Locus ID 23164
UniProt ID Q6WCQ1
Cytogenetics 17p11.2
Protein Families Druggable Genome
MW 116.5 kDa
Summary Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_201274" in other vectors (4)
SKU Description Size Price
RC224291 MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 10 ug
$892.00
RC224291L3 Lenti-ORF clone of MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 10 ug
$1,192.00
RC224291L4 Lenti-ORF clone of MPRIP (mGFP-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 10 ug
$1,192.00
RG224291 MPRIP (tGFP-tagged) - Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2 10 ug
$1,092.00

file-alt Citations

A novel marker for Purkinje cells, KIAA0864 protein. An analysis based on a monoclonal antibody HFB-16 in developing human cerebellum
Nakamura, Yamamoto, Oda et al
J Histochem Cytochem (2005) 53 (4), 423-30 DOI: 10.1369/jhc.4A6499.2005
AI Summary: In this study, OriGene’s human KIAA0864 cDNA and protein expression vector were used to confirm the antigenicity of the KIAA0864 protein recognized by the HFB-16 monoclonal antibody, thus validating KIAA0864 as a novel marker for Purkinje cells in the developing human cerebellum.
Showing 1-1 of 1 papers.
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