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SETD3 (NM_199123) Human Untagged Clone

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SKU
SC307867
SETD3 (untagged)-Human SET domain containing 3 (SETD3), transcript variant 2
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SETD3
Synonyms C14orf154; hSETD3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC307867 representing NM_199123.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGTAAGAAGAGTCGAGTAAAAACTCAGAAATCTGGCACTGGTGCTACAGCAACTGTGTCACCAAAG
GAAATCTTGAACCTGACCAGTGAGCTGCTGCAGAAATGCAGCAGTCCGGCGCCTGGCCCAGGAAAAGAG
TGGGAAGAGTATGTGCAGATCCGGACTCTGGTTGAGAAAATACGGAAAAAGCAAAAAGGTCTGTCCGTT
ACTTTTGATGGAAAAAGAGAAGATTACTTTCCTGATCTAATGAAATGGGCCTCTGAAAATGGGGCTTCT
GTCGAGGGTTTTGAAATGGTTAACTTCAAAGAAGAGGGCTTTGGTTTGAGAGCAACAAGAGATATCAAG
GCAGAAGAATTGTTTTTATGGGTTCCACGAAAATTGCTAATGACTGTTGAATCTGCTAAAAATTCAGTG
TTGGGGCCCTTATATTCTCAAGACCGAATCCTTCAAGCCATGGGAAACATCGCACTGGCCTTTCATTTG
CTGTGTGAGCGAGCCAGCCCTAACTCCTTCTGGCAGCCCTATATTCAAACCCTCCCCAGTGAATATGAC
ACTCCTCTCTACTTTGAAGAAGATGAAGTTCGGTATCTTCAGTCCACACAAGCTATACATGATGTCTTC
AGCCAGTATAAAAACACAGCTCGACAGTACGCCTACTTCTATAAAGTCATCCAGACCCATCCTCATGCC
AACAAACTACCCTTGAAGGATTCTTTCACTTACGAGGACTACAGGTGGGCAGTCTCTTCTGTTATGACG
AGGCAAAACCAAATTCCCACAGAGGATGGTTCCCGCGTGACCCTGGCTCTGATTCCTTTATGGGATATG
TGTAACCACACCAACGGCCTGACACCAGAAGATTCCTTCGCTCTTGCTGTCGCCTCTGCCTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_199123
Insert Size 891 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199123.1
RefSeq Size 1441 bp
RefSeq ORF 891 bp
Locus ID 84193
UniProt ID Q86TU7
Cytogenetics 14q32.2
MW 33.7 kDa
Summary Protein-histidine N-methyltransferase that specifically mediates methylation of actin at 'His-73' (PubMed:30526847, PubMed:30626964, PubMed:30785395). Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (PubMed:30626964). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (PubMed:30626964, PubMed:30785395).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a unique C-terminus compared to isoform a.
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Documents
Resources
"NM_199123" in other vectors (4)
SKU Description Size Price
RC220767 SETD3 (Myc-DDK-tagged)-Human SET domain containing 3 (SETD3), transcript variant 2 10 ug
$300.00
RC220767L3 Lenti ORF clone of Human SET domain containing 3 (SETD3), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC220767L4 Lenti ORF clone of Human SET domain containing 3 (SETD3), transcript variant 2, mGFP tagged 10 ug
$600.00
RG220767 SETD3 (tGFP-tagged) - Human SET domain containing 3 (SETD3), transcript variant 2 10 ug
$500.00

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