PHACTR3 (NM_183246) Human Untagged Clone

SKU
SC307564
PHACTR3 (untagged)-Human phosphatase and actin regulator 3 (PHACTR3), transcript variant 3
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PHACTR3
Synonyms C20orf101; H17739; PPP1R123; SCAPIN1; SCAPININ
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC307564 representing NM_183246.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGACCAAACGCCCCCGGCGCGTCCTGAATATCTGGTCTCAGGGATTCGAACTCCCCCTGTGAGGAGG
AACAGCAAACTGGCCACCCTGGGCAGGATCTTCAAACCCTGGAAATGGAGGAAAAAGAAAAACGAAAAA
CTGAAGCAGACAACGTCAGCGCTGGAGAAGAAGATGGCCGGCAGGCAAGGCCGAGAGGAGCTCATCAAG
AAGGGGCTGCTGGAGATGATGGAGCAGGATGCTGAAAGCAAAACTTGCAACCCCGATGGAGGACCCCGA
TCTGTACAGAGTGAACCACCCACTCCCAAGTCGGAGACGCTGACTTCAGAAGATGCCCAGCCCGGAAGC
CCCTTGGCCACTGGGACGGACCAGGTCTCCCTGGACAAGCCACTGTCCTCAGCTGCCCACTTGGACGAT
GCAGGCCAAGCCACACTCTTCCAAGCCTCCAGCATGAAGAGTGCCGACCCTTCCCTCCGGGGCCAGCTC
TCCACACCCACGGGGTCTCCGCATCTCACCACGGTCCACCGGCCTCTTCCCCCAAGCCGCGTCATTGAG
GAGCTGCACAGGGCGCTGGCCACGAAGCACCGCCAGGACAGTTTTCAAGGAAGAGAAAGTAAAGGGTCT
CCAAAGAAGCGGCTGGATGTCCGTCTGTCGAGAACGTCCAGCGTGGAGCGGGGCAAGGAGAGGGAGGAG
GCTTGGAGCTTTGACGGGGCATTGGAGAACAAGCGAACTGCCGCTAAGGAATCTGAGGAGAACAAGGAG
AACCTGATCATAAATTCTGAACTCAAAGACGACTTGCTTTTGTATCAGGACGAGGAGGCGCTGAACGAC
TCCATTATTTCTGGAACACTGCCACGGAAATGCAAGAAGGAGCTCCTGGCCGTGAAGCTAAGGAACCGG
CCAAGCAAACAGGAACTAGAAGACCGGAACATTTTCCCCAGAAGGACTGATGAAGAAAGACAGGAGATC
CGGCAGCAGATCGAGATGAAGCTTTCCAAACGGCTGAGCCAAAGACCTGCCGTGGAAGAGCTGGAGAGA
AGAAATATCTTGAAACAAAGGAATGATCAGACAGAGCAGGAAGAAAGAAGAGAAATCAAGCAAAGATTG
ACAAGAAAGCTTAATCAGAGACCCACTGTTGATGAATTAAGAGACAGAAAAATTCTGATACGATTCAGT
GATTACGTGGAAGTAGCAAAAGCGCAGGACTATGACAGGAGGGCAGACAAACCCTGGACGAGACTGTCA
GCAGCAGATAAGGCAGCAATTCGTAAAGAATTAAATGAGTACAAAAGTAATGAAATGGAGGTACATGCA
TCAAGCAAGCACTTGACAAGATTCCACAGGCCATAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_183246
Insert Size 1347 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183246.1
RefSeq Size 2185 bp
RefSeq ORF 1347 bp
Locus ID 116154
UniProt ID Q96KR7
Cytogenetics 20q13.32-q13.33
MW 51.5 kDa
Summary This gene encodes a member of the phosphatase and actin regulator protein family. The encoded protein is associated with the nuclear scaffold in proliferating cells, and binds to actin and the catalytic subunit of protein phosphatase-1, suggesting that it functions as a regulatory subunit of protein phosphatase-1. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2013
Transcript Variant: This variant (3) uses an alternate 5'-terminal exon, lacks an in-frame exon in the central coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3, also known as scapinin-S) is shorter, compared to isoform 1.
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Documents
Resources
"NM_183246" in other vectors (4)
SKU Description Size Price
RC212083 PHACTR3 (Myc-DDK-tagged)-Human phosphatase and actin regulator 3 (PHACTR3), transcript variant 3 10 ug
$289.00 $457.00
RC212083L3 Lenti ORF clone of Human phosphatase and actin regulator 3 (PHACTR3), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC212083L4 Lenti ORF clone of Human phosphatase and actin regulator 3 (PHACTR3), transcript variant 3, mGFP tagged 10 ug
$757.00
RG212083 PHACTR3 (tGFP-tagged) - Human phosphatase and actin regulator 3 (PHACTR3), transcript variant 3 10 ug
$657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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