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SP8 (NM_182700) Human Untagged Clone

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SKU
SC307485
SP8 (untagged)-Human Sp8 transcription factor (SP8), transcript variant 1
  $521.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SP8
Synonyms BTD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC307485 representing NM_182700.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCAACTTCACTTCTAGGGGAAGAACCGAGGTTGGGATCGACTCCTCTGGCCATGCTTGCCGCTACC
TGTAATAAGATAGGCAGCCCCAGCCCGTCTCCCTCCTCCCTCTCGGACAGCTCTTCTTCCTTCGGCAAA
GGCTTCCACCCCTGGAAACGCTCCTCGTCCTCTTCTTCCGCCAGCTGCAACGTAGTGGGTTCCAGTCTC
TCAAGCTTCGGCGTGTCCGGGGCCTCCAGGAACGGCGGCTCGTCCTCGGCGGCTGCGGCGGCCGCGGCA
GCAGCCGCGGCTGCCGCGGCCCTGGTGTCCGACTCGTTCAGCTGCGGCGGCTCGCCTGGCTCCAGCGCC
TTCTCCCTCACCTCCAGCAGCGCCGCAGCCGCCGCCGCCGCCGCCGCAGCCGCCGCCTCCAGCTCGCCC
TTCGCCAACGACTACTCTGTTTTCCAGGCCCCCGGAGTTTCCGGGGGCAGCGGCGGCGGCGGCGGGGGC
GGCGGCGGCGGCTCCTCCGCGCACTCGCAGGACGGCTCCCACCAGCCGGTGTTCATCTCCAAGGTGCAC
ACCTCTGTGGACGGGCTGCAGGGCATCTACCCGCGGGTGGGCATGGCGCACCCGTACGAGTCGTGGTTT
AAGCCCTCGCACCCGGGCCTGGGTGCTGCGGGCGAGGTGGGCTCGGCCGGCGCCTCCAGCTGGTGGGAC
GTGGGGGCCGGCTGGATCGACGTGCAGAACCCGAACAGCGCGGCTGCGCTGCCCGGCTCGCTGCACCCT
GCCGCCGGGGGGCTCCAAACCTCGCTGCACTCGCCGCTCGGAGGCTACAACTCGGATTACTCGGGCCTG
AGTCACTCGGCCTTCAGCAGCGGCGCCTCCTCGCACCTGCTCAGCCCCGCCGGGCAGCACCTCATGGAC
GGCTTCAAGCCAGTGCTACCCGGCTCCTACCCGGACTCGGCCCCGTCGCCGCTGGCCGGCGCGGGGGGC
TCCATGTTGAGCGCTGGGCCTTCGGCGCCGCTGGGGGGCTCCCCGCGCTCCTCAGCTCGCCGCTACTCC
GGCCGCGCCACCTGCGACTGCCCCAACTGCCAGGAGGCAGAGCGGCTGGGCCCTGCCGGGGCGAGCTTG
CGGCGCAAGGGCCTGCACAGCTGCCACATCCCGGGCTGCGGCAAGGTGTACGGCAAGACTTCGCACCTC
AAGGCGCACCTGCGCTGGCACACGGGCGAGCGGCCCTTCGTGTGCAACTGGCTTTTCTGCGGCAAGCGC
TTCACGCGCTCCGACGAGCTGCAGCGGCACCTGCGGACCCACACCGGCGAGAAGCGCTTCGCCTGTCCA
GTTTGCAACAAGCGCTTCATGCGCAGCGACCACCTCAGCAAGCACGTGAAGACGCACAGTGGCGGCGGC
GGCGGCGGCGGCTCGGCGGGCTCGGGCAGCGGCGGCAAGAAGGGCAGCGACACCGACAGCGAGCACAGC
GCCGCGGGCAGCCCGCCCTGCCACTCCCCAGAGCTGCTGCAGCCCCCCGAGCCCGGGCACCGCAACGGC
CTAGAGTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_182700
Insert Size 1527 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182700.5
RefSeq Size 3643 bp
RefSeq ORF 1527 bp
Locus ID 221833
UniProt ID Q8IXZ3
Cytogenetics 7p21.1
MW 50.5 kDa
Summary The protein encoded by this gene is an SP family transcription factor that in mouse has been shown to be essential for proper limb development. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2011
Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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"NM_182700" in other vectors (4)
SKU Description Size Price
RC212880 SP8 (Myc-DDK-tagged)-Human Sp8 transcription factor (SP8), transcript variant 1 10 ug
$473.00
RC212880L3 Lenti ORF clone of Human Sp8 transcription factor (SP8), transcript variant 1, Myc-DDK-tagged 10 ug
$773.00
RC212880L4 Lenti ORF clone of Human Sp8 transcription factor (SP8), transcript variant 1, mGFP tagged 10 ug
$773.00
RG212880 SP8 (tGFP-tagged) - Human Sp8 transcription factor (SP8), transcript variant 1 10 ug
$673.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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