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DLC1 (NM_182643) Human Untagged Clone

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SKU
SC307473
DLC1 (untagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 1
  $1,439.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DLC1
Synonyms ARHGAP7; HP; p122-RhoGAP; STARD12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC307473 representing NM_182643.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCTGTAGCTATCAGAAAGAGAAGCTGGGAAGAACATGTGACCCACTGGATGGGACAGCCTTTTAAT
TCTGATGATCGTAACACAGCATGTCATCATGGACTAGTAGCTGACAGCTTGCAGGCAAGTATGGAAAAA
GATGCAACTCTAAATGTGGACCGCAAAGAGAAGTGTGTTTCACTACCTGACTGCTGTCATGGATCAGAG
CTGAGAGATTTTCCTGGGAGGCCAATGGGTCATCTTTCAAAGGATGTGGACGAAAATGACAGCCATGAA
GGTGAAGATCAGTTTCTTTCTCTGGAAGCCAGCACAGAAACACTAGTGCATGTTTCTGATGAGGATAAC
AATGCTGATTTATGCCTTACAGATGATAAACAGGTTTTAAATACCCAAGGGCAGAAAACATCAGGCCAA
CATATGATCCAAGGAGCAGGCTCCTTAGAAAAGGCACTGCCCATCATACAAAGTAACCAAGTTTCTTCT
AACTCCTGGGGAATAGCTGGTGAAACTGAATTAGCACTGGTAAAAGAAAGTGGGGAGAGAAAAGTTACT
GACTCTATAAGTAAAAGCCTGGAGCTTTGCAATGAAATAAGCTTAAGTGAAATAAAAGATGCACCCAAA
GTAAATGCAGTGGATACTTTGAACGTGAAAGATATTGCACCTGAGAAACAATTGCTTAACTCTGCTGTA
ATTGCTCAGCAACGAAGGAAACCTGACCCCCCTAAAGATGAAAATGAAAGAAGCACCTGCAATGTAGTA
CAAAATGAGTTCTTGGATACTCCTTGCACAAACAGAGGACTGCCATTATTAAAAACAGATTTTGGAAGC
TGCCTTCTGCAGCCTCCTTCCTGCCCCAATGGAATGTCAGCTGAAAATGGCCTGGAGAAGAGTGGTTTT
TCACAACATCAAAACAAAAGTCCACCAAAGGTCAAGGCAGAAGATGGCATGCAGTGTTTACAATTAAAG
GAGACCCTGGCCACCCAGGAACCCACAGATAACCAAGTCAGACTTCGTAAGAGAAAGGAAATAAGAGAA
GATCGAGATAGGGCGCGGCTGGACTCCATGGTGCTGCTGATTATGAAACTGGACCAGCTTGATCAGGAC
ATAGAAAATGCCCTCAGCACCAGCTCCTCTCCATCAGGCACACCAACAAACCTGCGGCGGCACGTTCCT
GATCTGGAATCAGGATCTGAAAGTGGAGCAGATACCATTTCAGTAAATCAGACACGAGTAAATTTGTCT
TCTGACACTGAGTCCACGGACCTCCCATCTTCCACTCCAGTAGCCAATTCTGGAACCAAACCCAAGACT
ACGGCTATTCAAGGTATTTCAGAGAAGGAAAAGGCTGAAATTGAAGCCAAGGAAGCTTGTGATTGGCTA
CGGGCAACTGGTTTCCCCCAGTATGCACAGCTTTATGAAGATTTCCTGTTCCCCATCGATATTTCCTTG
GTCAAGAGAGAGCATGATTTTTTGGACAGAGATGCCATTGAGGCTCTATGCAGGCGTCTAAATACTTTA
AACAAATGTGCGGTGATGAAGCTAGAAATTAGTCCTCATCGGAAACGAAGTGACGATTCAGACGAGGAT
GAGCCTTGTGCCATCAGTGGCAAATGGACTTTCCAAAGGGACAGCAAGAGGTGGTCCCGGCTTGAAGAG
TTTGATGTCTTTTCTCCAAAACAAGACCTGGTCCCTGGGTCCCCAGACGACTCCCACCCGAAGGACGGC
CCCAGCCCCGGAGGCACGCTGATGGACCTCAGCGAGCGCCAGGAGGTGTCTTCCGTCCGCAGCCTCAGC
AGCACTGGCAGCCTCCCCAGCCACGCGCCCCCCAGCGAGGATGCTGCCACCCCCCGGACTAACTCCGTC
ATCAGCGTTTGCTCCTCCAGCAACTTGGCAGGCAATGACGACTCTTTCGGCAGCCTGCCCTCTCCCAAG
GAACTGTCCAGCTTCAGCTTCAGCATGAAAGGCCACGAAAAAACTGCCAAGTCCAAGACGCGCAGTCTG
CTGAAACGGATGGAGAGCCTGAAGCTCAAGAGCTCCCATCACAGCAAGCACAAAGCGCCCTCAAAGCTG
GGGTTGATCATCAGCGGGCCCATCTTGCAAGAGGGGATGGATGAGGAGAAGCTGAAGCAGCTCAACTGC
GTGGAGATCTCCGCCCTCAATGGCAACCGCATCAACGTCCCCATGGTACGAAAGAGGAGCGTTTCCAAC
TCCACGCAGACCAGCAGCAGCAGCAGCCAGTCGGAGACCAGCAGCGCGGTCAGCACGCCCAGCCCTGTT
ACGAGGACCCGGAGCCTCAGTGCGTGCAACAAGCGGGTGGGCATGTACTTAGAGGGCTTCGATCCTTTC
AATCAGTCAACATTTAACAACGTGGTGGAGCAGAACTTTAAGAACCGCGAGAGCTACCCAGAGGACACG
GTGTTCTACATCCCTGAAGATCACAAGCCTGGCACTTTCCCCAAAGCTCTCACCAATGGCAGTTTCTCC
CCCTCGGGGAATAACGGCTCTGTGAACTGGAGGACGGGAAGCTTCCACGGCCCTGGCCACATCAGCCTC
AGGAGGGAAAACAGTAGCGACAGCCCCAAGGAACTGAAGAGACGCAATTCTTCCAGCTCCATGAGCAGC
CGCCTGAGCATCTACGACAACGTGCCGGGCTCCATCCTCTACTCCAGTTCAGGGGACCTGGCGGATCTG
GAGAACGAGGACATCTTCCCCGAGCTGGACGACATCCTCTACCACGTGAAGGGGATGCAGCGGATAGTC
AATCAGTGGTCGGAGAAGTTTTCTGATGAGGGAGATTCGGACTCAGCCCTGGACTCGGTCTCTCCCTGC
CCGTCCTCTCCAAAACAGATACACCTGGATGTGGACAACGACCGAACCACACCCAGCGACCTGGACAGC
ACAGGCAACTCCCTGAATGAACCGGAAGAGCCCTCCGAGATCCCGGAAAGAAGGGATTCTGGGGTTGGG
GCTTCCCTAACCAGGTCCAACAGGCACCGACTGAGATGGCACAGTTTCCAGAGCTCACATCGGCCAAGC
CTCAACTCTGTATCACTACAGATTAACTGCCAGTCTGTGGCCCAGATGAACCTGCTGCAGAAATACTCA
CTCCTAAAGCTAACGGCCCTGCTGGAGAAATACACACCTTCTAACAAGCATGGTTTTAGCTGGGCCGTG
CCCAAGTTCATGAAGAGGATCAAGGTTCCAGACTACAAGGACCGGAGTGTGTTTGGGGTCCCACTGACG
GTCAACGTGCAGCGCACAGGACAACCGTTGCCTCAGAGCATCCAGCAGGCCATGCGATACCTCCGGAAC
CATTGTTTGGATCAGGTTGGGCTCTTCAGAAAATCGGGGGTCAAGTCCCGGATTCAGGCTCTGCGCCAG
ATGAATGAAGGTGCCATAGACTGTGTCAACTACGAAGGACAGTCTGCTTATGACGTGGCAGACATGCTG
AAGCAGTATTTTCGAGATCTTCCTGAGCCACTAATGACGAACAAACTCTCGGAAACCTTTCTACAGATC
TACCAATATGTGCCCAAGGACCAGCGCCTGCAGGCCATCAAGGCTGCCATCATGCTGCTGCCTGACGAG
AACCGGGAGGTTCTGCAGACCCTGCTTTATTTCCTGAGCGATGTCACAGCAGCCGTAAAAGAAAACCAG
ATGACCCCAACCAACCTGGCCGTGTGCTTAGCGCCTTCCCTCTTCCATCTCAACACCCTGAAGAGAGAG
AATTCCTCTCCCAGGGTAATGCAAAGAAAACAAAGTTTGGGCAAACCAGATCAGAAAGATTTGAATGAA
AACCTAGCTGCCACTCAAGGGCTGGCCCATATGATCGCCGAGTGCAAGAAGCTTTTCCAGGTTCCCGAG
GAAATGAGCCGATGTCGTAATTCCTATACCGAACAAGAGCTGAAGCCCCTCACTCTGGAAGCACTCGGG
CACCTGGGTAATGATGACTCAGCTGACTACCAACACTTCCTCCAGGACTGTGTGGATGGCCTGTTTAAA
GAAGTCAAAGAGAAGTTTAAAGGCTGGGTCAGCTACTCCACTTCGGAGCAGGCTGAGCTGTCCTATAAG
AAGGTGAGCGAAGGACCCCCTCTGAGGCTTTGGAGGTCAGTCATTGAAGTCCCTGCTGTGCCAGAGGAA
ATCTTAAAGCGCCTACTTAAAGAACAGCACCTCTGGGATGTAGACCTGTTGGATTCAAAAGTGATCGAA
ATTCTGGACAGCCAAACTGAAATTTACCAGTATGTCCAAAACAGTATGGCACCTCATCCTGCTCGAGAC
TACGTTGTTTTAAGAACCTGGAGGACTAATTTACCCAAAGGAGCCTGTGCCCTTTTACTAACCTCTGTG
GATCACGATCGCGCACCTGTGGTGGGTGTGAGGGTTAATGTGCTCTTGTCCAGGTATTTGATTGAACCC
TGTGGGCCAGGAAAATCCAAACTCACCTACATGTGCAGAGTTGACTTAAGGGGCCACATGCCAGAATGG
TACACAAAATCTTTTGGACATTTGTGTGCAGCTGAAGTTGTAAAGATCCGGGATTCCTTCAGTAACCAG
AACACTGAAACCAAAGACACCAAATCTAGGTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_182643
Insert Size 4587 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182643.2
RefSeq Size 7479 bp
RefSeq ORF 4587 bp
Locus ID 10395
UniProt ID Q96QB1
Cytogenetics 8p22
MW 170.6 kDa
Summary This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.provided by RefSeq, Apr 2010
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variant 1 represents a transcript that is found in low abundance. Variants 1 and 6 both encode the same isoform (1).
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Documents
Resources
"NM_182643" in other vectors (6)
SKU Description Size Price
RC208691 DLC1 (Myc-DDK-tagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 1 10 ug
$1,307.00
RC208691L1 Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,607.00
RC208691L2 Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 1, mGFP tagged 10 ug
$1,607.00
RC208691L3 Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,607.00
RC208691L4 Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 1, mGFP tagged 10 ug
$1,607.00
RG208691 DLC1 (tGFP-tagged) - Human deleted in liver cancer 1 (DLC1), transcript variant 1 10 ug
$1,507.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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