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KRT25 (NM_181534) Human Untagged Clone

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SKU
SC307301
KRT25 (untagged)-Human keratin 25 (KRT25)
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol KRT25
Synonyms ARWH3; KRT24IRS1; KRT25A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC307301 representing NM_181534.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCTCTTCGACTTTCCAGTGCATCCAGGAGGTCCTGTCCTCGTCCCACCACTGGATCACTCAGACTC
TATGGTGGGGGAACCAGCTTTGGTACTGGAAATTCTTGTGGCATTTCAGGGATTGGAAGTGGCTTCTCT
AGTGCCTTCGGAGGCAGCTCATCGGGAGGAAACACAGGGGGAGGTAATCCCTGTGCTGGCTTCACTGTG
AATGAGCGGGGGCTCCTTTCTGGCAATGAGAAGGTGACCATGCAGAACCTCAATGACCGCCTGGCATCC
TACCTGGACAGTGTGCATGCTCTGGAGGAGGCCAACGCTGACCTGGAGCAGAAGATCAAGGGCTGGTAT
GAGAAATTTGGGCCTGGCTCTTGCCGTGGTCTTGATCATGACTATAGCAGATATTTCCCAATAATTGAT
GACCTTAAAAATCAGATCATCGCATCCACCACCAGCAATGCTAATGCTGTTCTGCAGATCGATAATGCC
AGGCTTACAGCTGATGATTTCAGACTCAAGTATGAAAATGAGCTGGCTCTTCACCAGAGTGTAGAGGCT
GATGTCAATGGGTTACGAAGAGTTTTGGATGAAATAACCCTGTGCAGAACAGATCTGGAGATTCAGTAT
GAAACCCTGAGTGAGGAGATGACTTACCTCAAAAAGAACCATAAAGAGGAAATGCAAGTTCTGCAGTGC
GCAGCTGGAGGCAACGTGAACGTGGAGATGAACGCAGCCCCCGGGGTGGACCTCACAGTTCTGCTGAAC
AACATGCGAGCTGAGTACGAAGCCCTTGCAGAGCAGAACCGCAGGGACGCGGAGGCCTGGTTCAACGAG
AAGAGCGCCTCCCTGCAGCAGCAGATCTCTGAGGATGTCGGAGCCACAACCTCAGCCCGGAATGAGCTG
ACTGAAATGAAGCGCACTCTTCAAACCCTGGAAATTGAACTTCAGTCTCTCCTAGCCACGAAACACTCC
CTGGAGTGCTCCTTGACAGAGACCGAGAGCAACTACTGTGCGCAGCTGGCGCAGATCCAGGCTCAGATC
GGGGCCCTGGAGGAGCAGCTGCACCAGGTCAGAACCGAGACCGAGGGCCAGAAGCTGGAGTATGAGCAG
CTCCTGGACATCAAGCTCCACCTGGAAAAAGAAATTGAGACCTACTGTCTCCTTATAGGAGGAGATGAT
GGAGCCTGTAAGTCTGGGGGTTACAAGTCTAAAGATTATGGATCTGGAAATGTGGGAAGTCAAGTCAAA
GACCCAGCCAAAGCCATAGTGGTTAAGAAAGTTCTTGAGGAGGTAGACCAACGCAGCAAAATACTTACC
ACCAGGCTCCACTCCCTGGAAGAGAAATCTCAAAGCAATTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_181534
Insert Size 1353 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181534.3
RefSeq Size 1703 bp
RefSeq ORF 1353 bp
Locus ID 147183
UniProt ID Q7Z3Z0
Cytogenetics 17q21.2
MW 49.3 kDa
Summary This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. provided by RefSeq, Jul 2009
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Documents
Resources
"NM_181534" in other vectors (4)
SKU Description Size Price
RC223582 KRT25 (Myc-DDK-tagged)-Human keratin 25 (KRT25) 10 ug
$457.00
RC223582L3 Lenti ORF clone of Human keratin 25 (KRT25), Myc-DDK-tagged 10 ug
$757.00
RC223582L4 Lenti ORF clone of Human keratin 25 (KRT25), mGFP tagged 10 ug
$757.00
RG223582 KRT25 (tGFP-tagged) - Human keratin 25 (KRT25) 10 ug
$657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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