LCE1A (NM_178348) Human Untagged Clone

SKU
SC307163
LCE1A (untagged)-Human late cornified envelope 1A (LCE1A)
  $165.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol LCE1A
Synonyms LEP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC307163 representing NM_178348.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCCTGCCAGCAGAGCCAGCAGCAGTGCCAGCCCCCTCCCAAGTGCACCCCCAAGTGCCCTCCCAAG
TGCCCCACTCCTAAGTGCCCCCCAAAGTGTCCCCCTAAGTGCCCTCCAGTCTCTTCCTGCTGCAGTGTC
AGCTCCGGAGGCTGCTGTGGCTCCAGCTCTGGGGGCGGCTGCAGCTCTGGGGGAGGTGGCTGCTGCCTG
AGCCACCACAGGCGCCACAGGTCCCACCGTCACAGACTCCAGAGCTCTGGCTGCTGCAGCCAGCCCTCG
GGAGGCTCCAGCTGCTGTGGAGGGGACAGCGGCCAGCACTCTGGAGGCTGCTGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_178348
Insert Size 333 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178348.2
RefSeq Size 625 bp
RefSeq ORF 333 bp
Locus ID 353131
UniProt ID Q5T7P2
Cytogenetics 1q21.3
MW 11 kDa
Summary LCE1A belongs to the late cornified envelope (LCE) gene cluster within the epidermal differentiation complex (EDC) on chromosome 1. The LCE cluster contains multiple conserved genes that encode stratum corneum proteins, and these genes are expressed relatively late during fetal assembly of the skin cornified envelope (Jackson et al., 2005 PubMed 15854049). For further information on the LCE gene cluster, see GENE FAMILY below.supplied by OMIM, Feb 2009
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Documents
Resources
"NM_178348" in other vectors (4)
SKU Description Size Price
RC213404 LCE1A (Myc-DDK-tagged)-Human late cornified envelope 1A (LCE1A) 10 ug
$150.00
RC213404L3 Lenti ORF clone of Human late cornified envelope 1A (LCE1A), Myc-DDK-tagged 10 ug
$450.00
RC213404L4 Lenti ORF clone of Human late cornified envelope 1A (LCE1A), mGFP tagged 10 ug
$450.00
RG213404 LCE1A (tGFP-tagged) - Human late cornified envelope 1A (LCE1A) 10 ug
$350.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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