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KRT74 (NM_175053) Human Untagged Clone

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SKU
SC306983
KRT74 (untagged)-Human keratin 74 (KRT74)
  $543.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol KRT74
Synonyms ADWH; HTSS2; HYPT3; K6IRS4; KRT5C; KRT6IRS4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC306983 representing NM_175053.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGTCGGCAACTGAACATCAAGTCCAGTGGTGACAAGGGCAACTTCAGTGTGCATTCGGCAGTGGTG
CCAAGGAAGGCTGTGGGTAGCCTGGCTTCTTACTGTGCAGCTGGCAGAGGGGCTGGCGCTGGCTTTGGC
AGTCGGAGCCTCTATAGCCTTGGAGGGAATCGGCGTATTTCTTTCAATGTGGCTGGTGGCGGCGTTCGG
GCTGGAGGTTACGGCTTCAGGCCTGGCTCTGGGTATGGAGGGGGCCGGGCCAGTGGCTTTGCTGGCAGT
ATGTTTGGCAGTGTGGCCCTGGGGCCTGCATGTTTGTCTGTGTGCCCACCTGGGGGCATCCACCAGGTC
ACTGTCAACAAGAGCCTCTTGGCCCCCCTCAACGTGGAGCTGGACCCTGAGATCCAGAAGGTGCGCGCC
CAGGAGCGGGAACAGATCAAGGTGCTGAACGACAAGTTCGCCTCCTTCATTGACAAGGTACGCTTCCTA
GAGCAGCAGAACCAGGTTCTAGAAACCAAGTGGGAGCTGCTGCAGCAGCTGGACCTGAACAACTGCAAG
AAGAACCTGGAGCCCATCCTTGAGGGCTACATCAGCAACCTGCGGAAGCAGCTGGAGACACTGTCTGGG
GACAGGGTGAGGCTGGACTCGGAGCTGAGAAGCATGAGGGATCTGGTGGAGGACTATAAGAAGAGATAT
GAAGTGGAGATTAACCGGCGCACGACAGCAGAGAATGAGTTTGTGGTGCTTAAGAAGGATGCAGATGCA
GCCTACGCAGTCAAGGTGGAGCTTCAGGCCAAAGTGGACTCACTGGACAAAGAAATCAAGTTCCTCAAG
TGTCTGTATGATGCAGAGATCGCTCAGATCCAGACTCACGCCAGTGAGACCTCTGTCATCCTGTCCATG
GACAACAACCGGGACCTGGACCTTGACAGCATCATCGCTGAGGTCCGCATGCATTATGAGGAGATCGCC
CTGAAGAGCAAGGCCGAGGCCGAGGCCCTGTACCAGACCAAGATCCAGGAGCTGCAGCTGGCAGCCAGT
CGGCATGGTGACGACCTGAAACACACCAGGAGCGAGATGGTGGAGCTGAACCGGCTCATCCAGAGGATC
CGGTGTGAGATCGGGAATGTGAAGAAGCAGCGTGCCAGCCTGGAGACGGCCATCGCTGACGCTGAGCAG
CGGGGAGACAATGCCCTGAAGGATGCCCAGGCCAAGCTGGATGAGCTGGAGGGCGCCCTGCACCAGGCC
AAGGAGGAGCTGGCGCGGATGCTGCGCGAGTACCAGGAGCTCATGAGCCTGAAACTGGCCCTGGACATG
GAGATTGCCACCTACCGCAAGCTGCTGGAGGGCGAGGAGTGCAGGATGTCTGGTGAGAATCCATCCTCT
GTGAGCATCTCTGTCATCAGCAGTAGCAGCTACAGCTACCACCACCCCAGCTCTGCGGGTGTTGACCTT
GGGGCCAGCGCTGTGGCAGGCAGCTCTGGCAGCACCCAGAGCGGGCAGACCAAGACCACAGAGGCGCGA
GGGGGAGACCTCAAGGACACCCAGGGCAAGAGCACCCCAGCCAGCATCCCAGCAAGGAAAGCCACCCGC
TAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_175053
Insert Size 1590 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175053.3
RefSeq Size 2799 bp
RefSeq ORF 1590 bp
Locus ID 121391
UniProt ID Q7RTS7
Cytogenetics 12q13.13
MW 57.9 kDa
Summary Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This protein belongs to a family of keratins that are specifically expressed in the inner root sheath of hair follicles.provided by RefSeq, Jun 2009
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"NM_175053" in other vectors (4)
SKU Description Size Price
RC218329 KRT74 (Myc-DDK-tagged)-Human keratin 74 (KRT74) 10 ug
$492.00
RC218329L3 Lenti ORF clone of Human keratin 74 (KRT74), Myc-DDK-tagged 10 ug
$792.00
RC218329L4 Lenti ORF clone of Human keratin 74 (KRT74), mGFP tagged 10 ug
$792.00
RG218329 KRT74 (tGFP-tagged) - Human keratin 74 (KRT74) 10 ug
$692.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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