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HS3ST5 (NM_153612) Human Untagged Clone

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SKU
SC306625
HS3ST5 (untagged)-Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5)
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HS3ST5
Synonyms 3-OST-5; 3OST5; HS3OST5; NBLA04021
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC306625 representing NM_153612.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTATTCAAACAGCAGGCGTGGCTGAGACAGAAGCTCCTGGTGCTGGGAAGCCTTGCCGTTGGGAGT
CTCCTGTATCTAGTCGCCAGAGTTGGGAGCTTGGATAGGCTACAACCCATTTGCCCCATTGAAGGTCGA
CTGGGTGGAGCCCGCACTCAGGCTGAATTCCCACTTCGCGCCCTGCAGTTTAAGCGTGGCCTGCTGCAC
GAGTTCCGGAAGGGCAACGCTTCCAAGGAGCAGGTTCGCCTCCATGACCTGGTCCAGCAGCTCCCCAAG
GCCATTATCATTGGGGTGAGGAAAGGAGGCACAAGGGCCCTGCTTGAAATGCTGAACCTACATCCGGCA
GTAGTCAAAGCCTCTCAAGAAATCCACTTTTTTGATAATGATGAGAATTATGGTAAGGGCATTGAGTGG
TATAGGAAAAAGATGCCTTTTTCCTACCCTCAGCAAATCACAATTGAAAAGAGCCCAGCATATTTTATC
ACAGAGGAGGTTCCAGAAAGGATTTACAAAATGAACTCATCCATCAAGTTGTTGATCATTGTCAGGGAG
CCAACCACAAGAGCTATTTCTGATTATACTCAGGTGCTAGAGGGGAAGGAGAGGAAGAACAAAACTTAT
TACAAGTTTGAGAAGCTGGCCATAGACCCTAATACATGCGAAGTGAACACAAAATACAAAGCAGTAAGA
ACCAGCATCTACACCAAACATCTGGAAAGGTGGTTGAAATACTTTCCAATTGAGCAATTTCATGTCGTC
GATGGAGATCGCCTCATCACGGAACCTCTGCCAGAACTTCAGCTCGTGGAGAAGTTCCTAAATCTGCCT
CCAAGGATAAGTCAATACAATTTATACTTCAATGCTACCAGAGGGTTTTACTGCTTGCGGTTTAATATT
ATCTTTAATAAGTGCCTGGCGGGCAGCAAGGGGCGCATTCATCCAGAGGTGGACCCCTCTGTCATTACT
AAATTGCGCAAATTCTTTCATCCTTTTAATCAAAAATTTTACCAGATCACTGGGAGGACATTGAACTGG
CCCTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_153612
Insert Size 1041 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153612.3
RefSeq Size 2744 bp
RefSeq ORF 1041 bp
Locus ID 222537
UniProt ID Q8IZT8
Cytogenetics 6q21-q22.1
Protein Families Transmembrane
Protein Pathways Heparan sulfate biosynthesis
MW 40.4 kDa
Summary HS3ST5 belongs to a group of heparan sulfate 3-O-sulfotransferases (EC 2.8.2.23) that transfer sulfate from 3-prime-phosphoadenosine 5-prime phosphosulfate (PAPS) to heparan sulfate and heparin (Mochizuki et al., 2003 PubMed 12740361).supplied by OMIM, Mar 2008
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"NM_153612" in other vectors (4)
SKU Description Size Price
RC210876 HS3ST5 (Myc-DDK-tagged)-Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5) 10 ug
$457.00
RC210876L3 Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5), Myc-DDK-tagged 10 ug
$757.00
RC210876L4 Lenti ORF clone of Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5), mGFP tagged 10 ug
$757.00
RG210876 HS3ST5 (tGFP-tagged) - Human heparan sulfate (glucosamine) 3-O-sulfotransferase 5 (HS3ST5) 10 ug
$489.00 $657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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