PGBD4 (NM_152595) Human Untagged Clone

SKU
SC306454
PGBD4 (untagged)-Human piggyBac transposable element derived 4 (PGBD4)
$600.00
3 Weeks*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PGBD4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC306454 representing NM_152595.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCAAATCCTAGAAAACGTAGCATTCCTATGCGTGATAGTAATACCGGTCTCGAACAGTTGTTGGCT
GAAGATTCATTTGATGAATCTGATTTTTCGGAAATAGATGATTCTGATAATTTTTCGGATAGTGCTTTA
GAAGCCGATAAGATCAGGCCTCTGTCCCATTTAGAATCTGATGGAAAGAGCTCTACATCAAGTGACTCA
GGGCGCTCCATGAAATGGTCAGCTCGTGCTATGATTCCACGTCAAAGGTATGACTTTACCGGCACACCT
GGCAGAAAAGTCGATGTCAGTGATATCACTGACCCATTGCAGTATTTTGAACTGTTCTTTACTGAGGAA
TTAGTTTCAAAAATTACTAGAGAAACAAATGCCCAAGCTGCCTTGTTGGCTTCAAAGCCACCGGGTCCG
AAAGGATTTTCGCGAATGGATAAATGGAAAGACACTGACAATGACGAGCTCAAAGTCTTTTTTGCAGTA
ATGTTACTGCAAGGTATTGTGCAGAAACCTGAGCTGGAGATGTTTTGGTCAACAAGGCCTCTTTTGGAT
ACACCTTATCTCAGGCAAATTATGACTGGTGAAAGATTTTTACTTTTGTTTCGGTGCCTGCATTTTGTC
AACAATTCTTCTATATCTGCTGGTCAATCAAAGGCCCAGATTTCATTGCAGAAGATCAAACCTGTGTTC
GACTTTCTTGTAAATAAATTTTCCACTGTATATACTCCAAACAGAAACATTGCAGTTGATGAATCACTG
ATGCTGTTCAAGGGGCCATTAGCTATGAAGCAGTACCTCCCGACAAAACGAGTACGATTTGGTCTGAAG
CTATATGTACTTTGTGAAAGTCAGTCTGGTTATGTGTGGAATGCGCTTGTTCACACAGGGCCTGGCATG
AATTTGAAAGATTCAGCGGATGGCCTGAAATCATCACGCATTGTTCTTACCTTGGTCAATGACCTTCTT
GGCCAAGGGTATTGTGTCTTCCTCGATAACTTTAATATATCTCCCATGCTTTTCAGAGAATTACATCAA
AATAGGACTGATGCAGTTGGGACAGCTCGTTTGAACAGAAAACAGATTCCAAATGATCTGAAAAAAAGG
ATTGCAAAGGGGACGACTGTAGCCAGATTCTGTGGTGAACTTATGGCACTGAAATGGTGTGACGGCAAG
GAGGTGACAATGTTGTCAACATTCCACAATGATACTGTGATTGAAGTAAACAATAGAAATGGAAAGAAA
ACTAAAAGGCCACGTGTCATTGTGGATTATAACGAGAATATGGGAGCAGTGGACTCGGCTGATCAAATG
CTTACTTCTTATCCATCTGAGCGCAAAAGACACAAGGTTTGGTATAAGAAATTCTTTCACCATCTTCTA
CACATTACAGTGCTGAACTCCTACATCCTGTTCAAGAAGGATAATCCTGAGCACACGATGAGCCATATA
AACTTCAGACTGGCATTGATTGAAAGAATGCTGGAAAAGCATCACAAGCCAGGGCAGCAACATCTTCGA
GGTCGTCCTTGCTCCGATGATGTCACACCTCTTCGTCTGTCTGGAAGACATTTCCCCAAGAGCATACCA
GCAACGTCCGGGAAACAGAATCCAACTGGTCGCTGCAAAATTTGCTGCTCCCAATACGACAAGGATGGC
AAGAAGATCCGGAAAGAAACGCGCTATTTTTGTGCCGAATGTGATGTTCCGCTTTGTGTTGTTCCGTGC
TTTGAAATTTACCACACGAAAAAAAATTATTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI
ACCN NM_152595
Insert Size 1758 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152595.4
RefSeq Size 2318 bp
RefSeq ORF 1758 bp
Locus ID 161779
UniProt ID Q96DM1
Cytogenetics 15q14
MW 67 kDa
Summary The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. [provided by RefSeq, Jul 2008]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.