OLAH (NM_018324) Human Untagged Clone

SKU
SC304578
OLAH (untagged)-Human oleoyl-ACP hydrolase (OLAH), transcript variant 1
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol OLAH
Synonyms AURA1; SAST; TE2; THEDC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC304578 representing NM_018324.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGAGAGGAGACCAACCTAAGAGAACCAGGAATGAAAACATTTTCAACTGCTTATACAAAAACCCT
GAGGCAACTTTTAAGCTGATTTGCTTTCCCTGGATGGGAGGTGGCTCCACTCATTTTGCCAAATGGGGC
CAAGATACTCATGATTTGCTGGAAGAGACAGCATCTCACCATGTTGCCAAGGCTGGTCTCAAACTCCGG
CGCTCAAGTGATCCTCCTGCTTCAGCCTACCCATGTGCTGGCGTGAGCCACCGTAGGCGTGAGCCACCG
TGCCTGGCCAAAATTCTTGGTCTATTCTGGATTCTAATTTTTTTTATGCACTCCTTAAGGCTTCCTGGA
AGAGAAAGCAGAGTTGAAGAACCTCTTGAAAATGACATCTCCCAGTTAGTTGATGAAGTTGTTTGTGCT
CTGCAGCCAGTCATCCAGGATAAACCATTTGCATTTTTTGGCCACAGTATGGGATCCTACATTGCTTTT
AGGACTGCACTAGGTCTAAAAGAAAACAATCAACCAGAACCATTGCATTTATTTTTGTCAAGTGCAACT
CCTGTACATTCAAAGGCCTGGCATCGCATTCCCAAAGATGATGAATTGTCAGAAGAACAAATAAGTCAT
TACCTTATGGAATTTGGAGGCACCCCCAAGCATTTTGCTGAAGCCAAGGAATTTGTGAAACAATGTAGT
CCCATCATAAGGGCAGATCTGAACATTGTTAGAAGTTGCACCTCTAACGTACCATCTAAGGCTGTTCTT
TCCTGTGACTTGACATGTTTTGTTGGATCTGAAGACATAGCAAAGGACATGGAAGCCTGGAAAGATGTA
ACCAGTGGAAATGCTAAAATTTACCAGCTTCCAGGGGGTCACTTTTATCTTCTGGATCCTGCGAACGAG
AAATTAATCAAGAACTACATAATCAAGTGTCTAGAAGTATCATCGATATCCAATTTTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_018324
Insert Size 957 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018324.2
RefSeq Size 1834 bp
RefSeq ORF 957 bp
Locus ID 55301
UniProt ID Q9NV23
Cytogenetics 10p13
Protein Pathways Fatty acid biosynthesis, Metabolic pathways
MW 35.8 kDa
Summary Contributes to the release of free fatty acids from fatty acid synthase (FASN). Has broad substrate specificity, giving rise to a range of free fatty acids with chain lengths between 10 and 16 carbon atoms (C10 - C16).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_018324" in other vectors (4)
SKU Description Size Price
RC219627 OLAH (Myc-DDK-tagged)-Human oleoyl-ACP hydrolase (OLAH), transcript variant 1 10 ug
$300.00
RC219627L3 Lenti ORF clone of Human oleoyl-ACP hydrolase (OLAH), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC219627L4 Lenti ORF clone of Human oleoyl-ACP hydrolase (OLAH), transcript variant 1, mGFP tagged 10 ug
$600.00
RG219627 OLAH (tGFP-tagged) - Human oleoyl-ACP hydrolase (OLAH), transcript variant 1 10 ug
$500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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