PCDHB1 (NM_013340) Human Untagged Clone

SKU SC303997

PCDHB1 (untagged)-Human protocadherin beta 1 (PCDHB1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PCDHB1
• Synonyms: PCDH-BETA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303997 representing NM_013340.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCGGGTACGCGCAGAAAATCTTTGCAAAACAGGCAAGTGGGATCTCTTCTCATTTTTCTGTGCATA; TCTGTGGGGGATGCGACAACTATCCGCTATTCAGTGGCAGAGGAAATGGAGAGCGGCTCGTTTGTGGCC; AACGTAGCTAAGGACCTAGGACTGGAGGTAGGGAAGCTGGCTGCGCGCGGGGCGCGGCTGGTTTCCGAG; GGCAACAAAATGCATTTCCGGCTCCACCGCAAGACGGGAGATTTGTTTGTGAAGGAGAAACTGGATCGG; GAGTCACTTTGTGGCAAAGCCGACCCTTGTGTTCTGCACTTTGAAGTAGTCCTGGTGGAGCCGCTGCAG; TCCTTCCGGGCCGAGGTCAGGGTATTTGATATCAATGACAATGCCCCAGTTTTCCTAAACAAGGAGCCG; CTTTTAAAGATTCCGGAGAGCACCCCTTTGGGTTCACGTTTTCCTCTGCAGAGCGCCCAGGATCTGGAC; GTGGGCCTTAACGGTCTCCAGAACTACACCCTGAGTGCCAATGGGTATTTCCACCTGCACACCCGCTTC; TGCAGCCACGGGCCTAAATATGCTGAGCTGGTGCTGAACAAACCCCTGGACCGAGAGGAGCAGCCTGAA; GTCAACTTGACAATTACGGCGGTGGACGGCGGGTCCCCGCCTAAGTCTGGCACAGCTCACATCCACGTG; GTGGTTCTGGATGTCAACGACCACGTGCCCCAGTTCTCGCGACTGGTGTACAGAGCCCAGGTATCAGAG; AACAGCCCCAATGGCTCTTTGGTGGCCACGGTGACTGCCGTGGACCTAGACGAGGGCACCAACAAAGCG; ATAACTTACTCTTTAGCTCAAAACCCAGAAGCAATTCTCAAGACGTTTCAGATTGACCCTCAAAATGGA; GAAGTTCGACTAAGAGGACCCCTCGATTTTGAAGCCATTGAAACATACGACATTGACATTCAAGCTACA; GATGGTGGAGGCCTCTCTGCCCACAGCAAAGTCCTGGTAGAAGTGGTGGATGTGAATGACAATCCTCCC; GAAGTGATGGTCTCCTCTGTGTCCAGCCCACTCCCTGAAGACTCACCACCACAGACAGTAGTAGCCCTT; TTCACTATCAGAGACCGGGACATTCGAGTGGGAGGAAAAGTCACCTGCTTCCTCAGAGAAGACCTTCCC; TTTGTAATCAAACCTACATTTGGGAATTCTTACTCACTGGTCACTGACAGAAGCTTGGATCGGGAGGAG; GTCTCAGGCTATAATATCACCATTGTTGCCATGGATACTGGACCACCTAGCTTGTCTGCCGAGACTATG; ATAGAGGTGCTAATATCCGACGTTAATGACAATCCTCCAATATTTCGGGAAGATTCCTATATCTTGACT; GTTCGAGAAAACAACAGTCCTGCGGTTTTTATTGGCAAAGTCCATGCTGAGGATCTTGATTTGGGTGAG; AATGCCCAAATAACATATTCTCTGTTGCCTCCAAAAAACGGAGATCTTTCAGTCTTTGCTTACATATCC; ATAAATTCAGGCAATGGGAAGCTCTACGCGCTGAGAACCATGGATTATGAGGCCATTCAAGATTTTCAA; TTTGTGGTAAAGGCAACTGATGGGGGCTTCCTGTCACTGAGTAGCCAAGTTACTGTCAGAGTGGTTGTC; CTAGATGACAATGACAATCGTCCAATGATCTTATACCCACTGCAGAACGGCACCTTGCCCTGCAATGAC; CTGGTGCCCAGGTCTGCAGAGGCAGGCTACCTAGTGACCAAAGTGGTGGCTGTGGATGGTGACTCAGGT; CAGAATTCTTGGCTTTCATATCATCTACTTAAGGCCACTGACCTTGGGTTATTTTCTGTTCAAAGACAA; AATGGAGAAATCCATACATTAAGGCAGATATCTGAGAGAGACCCCATGATGCAGAAATTGATCATTCTT; GTTCAGGATCACGGCCAACCAGCTCTTTCCACTACTGTCTCACTCAACATCCTGCTGGTAGATGGCTTT; TCAGAGCCCTACCTGCAGTTCCAGGATCCAACCAAGCATTCTAGAAAGGTAAATCCATCCACTAAATAT; TTGGTCATTTCTCTGGTCATCCTTTCCTTTCTCTTTCTCCTCTCTGTCATAGTGATCTTCATTATACAT; GTCTACCAAAAGATTAAATATAGAGAAAAGTTCACAATTCAAGAGCATTTCTATGATGACTGTAATTTC; TCTAACAACCTGGTACAAGGACAAGGCAATGGATCCTTATCTCGGCCTTGTCCATATGAAATGTGTTCA; GCCACTGGCACTGGTAATAGTGAGTTTCGCTTTCTTAAGCGTTTTATGCCCAACTTCCCTTTCCCTCAT; GCCACTGGGGAGATAAAAATGGAGGCTGGCTCCAGTTTGCCCCCAAATTCTGATAGGAATAAGTCTCAG; AGATTAGAGGGCCATGACCAGGTATCTGATGACTATATGTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_013340
• Insert Size: 2457 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013340.3
• RefSeq Size: 2587 bp
• RefSeq ORF: 2457 bp
• Locus ID: 29930
• UniProt ID: Q9Y5F3
• Cytogenetics: 5q31.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 90.5 kDa
• Summary: This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq, Jul 2008]