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DKK1 (NM_012242) Human Untagged Clone

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SKU
SC303946
DKK1 (untagged)-Human dickkopf homolog 1 (Xenopus laevis) (DKK1)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DKK1
Synonyms DKK-1; SK
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_012242 edited
CCCTGCAGTCAGGACTCTGGGACCGCAGGGGGCTCCCGGACCCTGACTCTGCAGCCGAAC
CGGCACGGTTTCGTGGGGACCCAGGCTTGCAAAGTGACGGTCATTTTCTCTTTCTTTCTC
CCTCTTGAGTCCTTCTGAGATGATGGCTCTGGGCGCAGCGGGAGCTACCCGGGTCTTTGT
CGCGATGGTAGCGGCGGCTCTCGGCGGCCACCCTCTGCTGGGAGTGAGCGCCACCTTGAA
CTCGGTTCTCAATTCCAACGCTATCAAGAACCTGCCCCCACCGCTGGGCGGCGCTGCGGG
GCACCCAGGCTCTGCAGTCAGCGCCGCGCCGGGAATCCTGTACCCGGGCGGGAATAAGTA
CCAGACCATTGACAACTACCAGCCGTACCCGTGCGCAGAGGACGAGGAGTGCGGCACTGA
TGAGTACTGCGCTAGTCCCACCCGCGGAGGGGACGCAGGCGTGCAAATCTGTCTCGCCTG
CAGGAAGCGCCGAAAACGCTGCATGCGTCACGCTATGTGCTGCCCCGGGAATTACTGCAA
AAATGGAATATGTGTGTCTTCTGATCAAAATCATTTCCGAGGAGAAATTGAGGAAACCAT
CACTGAAAGCTTTGGTAATGATCATAGCACCTTGGATGGGTATTCCAGAAGAACCACCTT
GTCTTCAAAAATGTATCACACCAAAGGACAAGAAGGTTCTGTTTGTCTCCGGTCATCAGA
CTGTGCCTCAGGATTGTGTTGTGCTAGACACTTCTGGTCCAAGATCTGTAAACCTGTCCT
GAAAGAAGGTCAAGTGTGTACCAAGCATAGGAGAAAAGGCTCTCATGGACTAGAAATATT
CCAGCGTTGTTACTGTGGAGAAGGTCTGTCTTGCCGGATACAGAAAGATCACCATCAAGC
CAGTAATTCTTCTAGGCTTCACACTTGTCAGAGACACTAAACCAGCTATCCAAATGCAGT
GAACTCCTTTTATATAATAGATGCTATGAAAACCTTTTATGACCTTCATCAACTCAATCC
TAAGGATATACAAGTTCTGTGGTTTCAGTTAAGCATTCCAATAACACCTTCCAAAAAAAA
AAAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_012242 unedited
NAAGTCAACAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCTGCA
GTCAGGACTCTGGGACCGCAGGGGCTCCCGGACCCTGACTCTGCAGCCGAACCGGCACGG
TTTCGTGGGGACCCAGGCTTGCAAAGTGACGGTCATTTTCTCTTTCTTTCTCCCTCTTGA
GTCCTTCTGAGATGATGGCTCTGGGCGCAGCGGGAGCTACCCGGGTCTTTGTCGCGATGG
TAGCGGCGGCTCTCGGCGGCCACCCTCTGCTGGGAGTGAGCGCCACCTTGAACTCGGTTC
TCAATTCCAACGCTATCAAGAACCTGCCCCCACCGCTGGGCGGCGCTGCGGGGCACCCAG
GCTCTGCAGTCAGCGCCGCGCCGGGAATCCTGTACCCGGGCGGGAATAAGTACCAGACCA
TTGACAACTACCAGCCGTACCCGTGCGCAGAGGACGAGGAGTGCGGCACTGATGAGTACT
GCGCTAGTCCCACCCGCGGAGGGGACGCATGCGTGCAAATCTGTCTCGCCTGCAGGAAGC
GCCGAANACGCTGCATGCGTCACGCTATGTGCTGCCCCGGGAATTACTGCAAAAATGGAA
TATGTGTGTCTTCTGATCAAAATCATTTCCGAGGAGAAATTGANGAAACCATCACTGANA
GCTTTGGTAATGATCATAGCACCTTGGATGGGTATTCCAGAAGAACCACCTTGTCTTCAA
AAATGTATCACACCANAGGACAAGAAGGTTCTGTTTGTCTCCGGTCATCAGACTGTGCCT
CAAGATTGTGTTGTGCTAGACACTTCTGGGTCAAGATCTGNTAACCTGTCCTGAAAGAAG
GTCAAGTGTGTACCAAGCATAGGAGGAAAGGCTCTCATGGACTAGAAATATTCCAGCGTT
GNTACTGTGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_012242 unedited
NACTTGTATATCCTTAGGATTGAGTTGATGAAGGTCATAAAAGGTTTTCATAGCATCTAT
TATATAAAAGGAGTTCACTGCATTTGGATAGCTGGTTTAGTGTCTCTGACAAGTGTGAAG
CCTAGAAGAATTACTGGCTTGATGGTGATCTTTCTGTATCCGGCAAGACAGACCTTCTCC
ACAGTAACAACGCTGGAATATTTCTAGTCCATGAGAGCCTTTTCTCCTATGCTTGGTACA
CACTTGACCTTCTTTCAGGACAGGTTTACAGATCTTGGACCAGAAGTGTCTAGCACAACA
CAATCCTGAGGCACAGTCTGATGACCGGAGACAAACAGAACCTTCTTGTCCTTTGGTGTG
ATACATTTTTGAAGACAAGGTGGTTCTTCTGGAATACCCATCCAAGGTGCTATGATCATT
ACCAAAGCTTTCAGTGATGGTTTCCTCAATTTCTCCTCGGAAATGATTTTGATCAGAAGA
CACACATATTCCATTTTTGCAGTAATTCCCGGGGCAGCACATAGCGTGACGCATGCAGCG
TTTTCGGCGCTTCCTGCAGGCGAGACAGATTTGCACGCCTGCGTCCCCTCCGCGGGTGGG
ACTAGCGCAGTACTCATCAGTGCCGCACTCCTCGTCCTCTGCGCACGGGTACGGCTGGTA
GTTGTCAATGGTCTGGTACTTATTCCCGCCCGGGTACAGGATTCCCGGCGCGGCGCTGAC
TGCAGAGCCTGGGTGCCCCGCAGCGCCGCCCCAGCGTGGGGGCAGGTTCTTGATAGCGTT
GGAATTGAGAACCGAGTTCAAGGTGGCGCTCACTCCCAGCAGAGGGTGGCCGCCGAGAGC
CGCCGCTACCATCGCGACAAAACCCGGGTAGCTCCCGCTGCGCCCAGAACATCA
Restriction Sites Please inquire |
ACCN NM_012242
Insert Size 1103 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012242.2, NP_036374.1
RefSeq Size 1815 bp
RefSeq ORF 801 bp
Locus ID 22943
UniProt ID O94907
Cytogenetics 10q21.1
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
Protein Pathways Wnt signaling pathway
Summary This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. provided by RefSeq, Sep 2017
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Documents
Resources
"NM_012242" in other vectors (6)
SKU Description Size Price
RC200094 DKK1 (Myc-DDK-tagged)-Human dickkopf homolog 1 (Xenopus laevis) (DKK1) 10 ug
$300.00
RC200094L1 Lenti ORF clone of Human dickkopf homolog 1 (Xenopus laevis) (DKK1), Myc-DDK-tagged 10 ug
$600.00
RC200094L2 Lenti ORF clone of Human dickkopf homolog 1 (Xenopus laevis) (DKK1), mGFP tagged 10 ug
$600.00
RC200094L3 Lenti ORF clone of Human dickkopf homolog 1 (Xenopus laevis) (DKK1), Myc-DDK-tagged 10 ug
$600.00
RC200094L4 Lenti ORF clone of Human dickkopf homolog 1 (Xenopus laevis) (DKK1), mGFP tagged 10 ug
$600.00
RG200094 DKK1 (tGFP-tagged) - Human dickkopf homolog 1 (Xenopus laevis) (DKK1) 10 ug
$489.00 $500.00

file-alt Citations

Bone marrow stromal cells create a permissive microenvironment for myeloma development: a new stromal role for Wnt inhibitor Dkk1
Fowler, Mundy, Lwin et al
Cancer Res. (2012) 72 (9), 2183-9 DOI: 10.1158/0008-5472.CAN-11-2067
Cited by 7
AI Summary: OriGene's full-length Dkk1 cDNA was used to transfect ST2 bone marrow stromal cells, enabling the researchers to study how increased Dkk1 expression in these cells influences myeloma development and bone disease in their mouse model.
Snippet: 14M1 BMSCs were transfected with 1 μg Dkk1shRNA or scrambled control (Santa Cruz Biotechnology) using short hairpin RNA (shRNA) transfection reagent ... ST2 BMSCs were transfected with 250 ng full-length Dkk1 cDNA (OriGene) or empty vector control according to the
A decrease in DKK1, a WNT inhibitor, contributes to placental lipid accumulation in an obesity-prone rat model
Strakovsky, Pan
Biol. Reprod. (2012) 86 (3), 81 DOI: 10.1095/biolreprod.111.094482
Cited by 56
AI Summary: OriGene’s transfection-ready DKK1 DNA (pCMV6-XL5 vector, catalog no. SC117943) was used to overexpress DKK1 in JEG3 cells, enabling the study of DKK1’s role in regulating placental lipid accumulation and metabolism.
Snippet: Treatment media without fatty acids was supplemented with additional bovine serum albumin to maintain the same osmolarity. Overexpression of DKK1 in JEG3 Cells. Transfection-ready DKK1 DNA inserted into a pCMV6-XL5 vector was purchased from OriGene (catalog no.
SOX2 has a crucial role in the lineage determination and proliferation of mesenchymal stem cells through Dickkopf-1 and c-MYC
Park, Seo, So et al
Cell Death Differ. (2012) 19 (3), 534-45 DOI: 10.1038/cdd.2011.137
Cited by 125
AI Summary: OriGene products, specifically the DKK1 plasmid vector (SC303946) and the control vector (PCMV6XL5), were used to manipulate DKK1 expression in human mesenchymal stem cells, enabling the authors to assess the role of DKK1 in SOX2-regulated differentiation and proliferation.
Snippet: characterization analyses. The DKK1 recovery assay was conducted using a DKK1 plasmid vector (number SC303946;OriGene Technologies, Rockville, MD, USA) and a control vector (number PCMV6XL5;OriGene Technologies). The
FigureFigureFigure
A decrease in dkk1, a Wnt inhibitor, contributes to placental and fetal lipid accumulation in an obesity-prone rat model
Strakovsky
Thesis (2011)
Snippet: Transfection-ready Dickkopf homolog 1 (Dkk1) DNA inserted into a pCMV6-XL5 vector was purchased from OriGene (Catalog #SC303946, mRNA NCBI Reference Sequence NM_012242.2, Rockville, MD)
Showing 1-4 of 4 papers.
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