DKK1 (NM_012242) Human Untagged Clone

SKU SC303946

DKK1 (untagged)-Human dickkopf homolog 1 (Xenopus laevis) (DKK1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DKK1
• Synonyms: DKK-1; SK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012242 edited; CCCTGCAGTCAGGACTCTGGGACCGCAGGGGGCTCCCGGACCCTGACTCTGCAGCCGAAC; CGGCACGGTTTCGTGGGGACCCAGGCTTGCAAAGTGACGGTCATTTTCTCTTTCTTTCTC; CCTCTTGAGTCCTTCTGAGATGATGGCTCTGGGCGCAGCGGGAGCTACCCGGGTCTTTGT; CGCGATGGTAGCGGCGGCTCTCGGCGGCCACCCTCTGCTGGGAGTGAGCGCCACCTTGAA; CTCGGTTCTCAATTCCAACGCTATCAAGAACCTGCCCCCACCGCTGGGCGGCGCTGCGGG; GCACCCAGGCTCTGCAGTCAGCGCCGCGCCGGGAATCCTGTACCCGGGCGGGAATAAGTA; CCAGACCATTGACAACTACCAGCCGTACCCGTGCGCAGAGGACGAGGAGTGCGGCACTGA; TGAGTACTGCGCTAGTCCCACCCGCGGAGGGGACGCAGGCGTGCAAATCTGTCTCGCCTG; CAGGAAGCGCCGAAAACGCTGCATGCGTCACGCTATGTGCTGCCCCGGGAATTACTGCAA; AAATGGAATATGTGTGTCTTCTGATCAAAATCATTTCCGAGGAGAAATTGAGGAAACCAT; CACTGAAAGCTTTGGTAATGATCATAGCACCTTGGATGGGTATTCCAGAAGAACCACCTT; GTCTTCAAAAATGTATCACACCAAAGGACAAGAAGGTTCTGTTTGTCTCCGGTCATCAGA; CTGTGCCTCAGGATTGTGTTGTGCTAGACACTTCTGGTCCAAGATCTGTAAACCTGTCCT; GAAAGAAGGTCAAGTGTGTACCAAGCATAGGAGAAAAGGCTCTCATGGACTAGAAATATT; CCAGCGTTGTTACTGTGGAGAAGGTCTGTCTTGCCGGATACAGAAAGATCACCATCAAGC; CAGTAATTCTTCTAGGCTTCACACTTGTCAGAGACACTAAACCAGCTATCCAAATGCAGT; GAACTCCTTTTATATAATAGATGCTATGAAAACCTTTTATGACCTTCATCAACTCAATCC; TAAGGATATACAAGTTCTGTGGTTTCAGTTAAGCATTCCAATAACACCTTCCAAAAAAAA; AAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012242 unedited; NAAGTCAACAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCTGCA; GTCAGGACTCTGGGACCGCAGGGGCTCCCGGACCCTGACTCTGCAGCCGAACCGGCACGG; TTTCGTGGGGACCCAGGCTTGCAAAGTGACGGTCATTTTCTCTTTCTTTCTCCCTCTTGA; GTCCTTCTGAGATGATGGCTCTGGGCGCAGCGGGAGCTACCCGGGTCTTTGTCGCGATGG; TAGCGGCGGCTCTCGGCGGCCACCCTCTGCTGGGAGTGAGCGCCACCTTGAACTCGGTTC; TCAATTCCAACGCTATCAAGAACCTGCCCCCACCGCTGGGCGGCGCTGCGGGGCACCCAG; GCTCTGCAGTCAGCGCCGCGCCGGGAATCCTGTACCCGGGCGGGAATAAGTACCAGACCA; TTGACAACTACCAGCCGTACCCGTGCGCAGAGGACGAGGAGTGCGGCACTGATGAGTACT; GCGCTAGTCCCACCCGCGGAGGGGACGCATGCGTGCAAATCTGTCTCGCCTGCAGGAAGC; GCCGAANACGCTGCATGCGTCACGCTATGTGCTGCCCCGGGAATTACTGCAAAAATGGAA; TATGTGTGTCTTCTGATCAAAATCATTTCCGAGGAGAAATTGANGAAACCATCACTGANA; GCTTTGGTAATGATCATAGCACCTTGGATGGGTATTCCAGAAGAACCACCTTGTCTTCAA; AAATGTATCACACCANAGGACAAGAAGGTTCTGTTTGTCTCCGGTCATCAGACTGTGCCT; CAAGATTGTGTTGTGCTAGACACTTCTGGGTCAAGATCTGNTAACCTGTCCTGAAAGAAG; GTCAAGTGTGTACCAAGCATAGGAGGAAAGGCTCTCATGGACTAGAAATATTCCAGCGTT; GNTACTGTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012242 unedited; NACTTGTATATCCTTAGGATTGAGTTGATGAAGGTCATAAAAGGTTTTCATAGCATCTAT; TATATAAAAGGAGTTCACTGCATTTGGATAGCTGGTTTAGTGTCTCTGACAAGTGTGAAG; CCTAGAAGAATTACTGGCTTGATGGTGATCTTTCTGTATCCGGCAAGACAGACCTTCTCC; ACAGTAACAACGCTGGAATATTTCTAGTCCATGAGAGCCTTTTCTCCTATGCTTGGTACA; CACTTGACCTTCTTTCAGGACAGGTTTACAGATCTTGGACCAGAAGTGTCTAGCACAACA; CAATCCTGAGGCACAGTCTGATGACCGGAGACAAACAGAACCTTCTTGTCCTTTGGTGTG; ATACATTTTTGAAGACAAGGTGGTTCTTCTGGAATACCCATCCAAGGTGCTATGATCATT; ACCAAAGCTTTCAGTGATGGTTTCCTCAATTTCTCCTCGGAAATGATTTTGATCAGAAGA; CACACATATTCCATTTTTGCAGTAATTCCCGGGGCAGCACATAGCGTGACGCATGCAGCG; TTTTCGGCGCTTCCTGCAGGCGAGACAGATTTGCACGCCTGCGTCCCCTCCGCGGGTGGG; ACTAGCGCAGTACTCATCAGTGCCGCACTCCTCGTCCTCTGCGCACGGGTACGGCTGGTA; GTTGTCAATGGTCTGGTACTTATTCCCGCCCGGGTACAGGATTCCCGGCGCGGCGCTGAC; TGCAGAGCCTGGGTGCCCCGCAGCGCCGCCCCAGCGTGGGGGCAGGTTCTTGATAGCGTT; GGAATTGAGAACCGAGTTCAAGGTGGCGCTCACTCCCAGCAGAGGGTGGCCGCCGAGAGC; CGCCGCTACCATCGCGACAAAACCCGGGTAGCTCCCGCTGCGCCCAGAACATCA;
• Restriction Sites: Please inquire
• ACCN: NM_012242
• Insert Size: 1103 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012242.2, NP_036374.1
• RefSeq Size: 1815 bp
• RefSeq ORF: 801 bp
• Locus ID: 22943
• UniProt ID: O94907
• Cytogenetics: 10q21.1
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Wnt signaling pathway
• Summary: This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]