DLEC1 (NM_007337) Human Untagged Clone
SKU
SC303915
DLEC1 (untagged)-Human deleted in lung and esophageal cancer 1 (DLEC1), transcript variant DLEC1-S3
$1,644.00
3 Weeks*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | DLEC1 |
| Synonyms | CFAP81; DLC-1; DLC1; F56; FAP81 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_007337 |
| Insert Size | 5337 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007337.3 |
| RefSeq Size | 5511 bp |
| RefSeq ORF | 5337 bp |
| Locus ID | 9940 |
| UniProt ID | Q9Y238 |
| Cytogenetics | 3p22.2 |
| MW | 197.9 kDa |
| Summary | The cytogenetic location of this gene is 3p21.3, and it is located in a region that is commonly deleted in a variety of malignancies. Down-regulation of this gene has been observed in several human cancers including lung, esophageal, renal tumors, and head and neck squamous cell carcinoma. In some cases, reduced expression of this gene in tumor cells is a result of aberrant promoter methylation. Several alternatively spliced transcripts have been observed that contain disrupted coding regions and likely encode nonfunctional proteins.provided by RefSeq, Mar 2016 Transcript Variant: This variant (DLEC1-S3) lacks an exon in the 3' coding region that results in a frameshift and use of a downstream stop codon, compared to variant DLEC1-N1. The encoded isoform (DLEC1-S3) has a longer, distinct C-terminus compared to the DLEC1-N1 isoform. There are currently no publicly available full-length transcripts supporting this variant, but it is supported by data in PMID:10213508. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007337" in other vectors (2)
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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