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PREPL (NM_006036) Human Untagged Clone

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SKU
SC303721
PREPL (untagged)-Human prolyl endopeptidase-like (PREPL), transcript variant 1
  $733.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PREPL
Synonyms CMS22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC303721 representing NM_006036.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCAGCAGAAGACCAAATTATTTCTCCAAGCTTTGAAGTATAGTATTCCTCACCTTGGAAAATGCATG
CAGAAACAGCATTTGAATCACTATAACTTCGCTGATCATTGTTACAATAGAATAAAATTGAAAAAATAT
CACCTAACCAAGTGTCTTCAGAATAAACCCAAGATATCAGAGTTAGCAAGAAACATCCCAAGTCGGAGC
TTCTCATGTAAGGATCTTCAGCCTGTTAAACAAGAAAACGAAAAACCCCTTCCAGAAAACATGGATGCA
TTTGAAAAAGTGAGAACAAAATTAGAAACACAGCCACAAGAAGAATATGAAATCATCAATGTGGAAGTT
AAACATGGTGGTTTTGTTTATTACCAAGAAGGTTGTTGCTTGGTTCGTTCCAAAGATGAAGAAGCAGAC
AATGATAATTATGAAGTTTTATTCAATTTGGAGGAACTTAAGTTAGACCAGCCCTTCATTGATTGTATC
AGAGTTGCTCCAGATGAAAAATATGTGGCTGCCAAGATAAGAACTGAAGATTCTGAAGCATCTACCTGT
GTAATTATAAAGCTCAGCGATCAGCCCGTAATGGAAGCTTCTTTCCCGAATGTGTCCAGTTTTGAATGG
GTAAAGGACGAGGAAGATGAAGATGTTTTATTCTACACCTTCCAGAGGAACCTTCGCTGTCATGACGTA
TATCGAGCCACTTTTGGTGATAACAAACGTAATGAACGCTTTTACACAGAAAAAGACCCAAGCTACTTT
GTTTTCCTTTATCTTACAAAAGACAGTCGTTTCCTCACCATAAATATTATGAACAAGACTACTTCTGAA
GTGTGGTTGATAGATGGCCTGAGCCCTTGGGACCCACCAGTACTTATCCAGAAGCGAATACATGGGGTC
CTTTACTATGTTGAACACAGAGATGATGAATTATACATTCTCACTAATGTTGGAGAACCTACAGAATTT
AAGCTAATGAGAACAGCGGCTGATACCCCTGCAATTATGAATTGGGATTTATTTTTTACAATGAAGAGA
AATACAAAAGTGATAGACTTGGACATGTTTAAGGATCACTGTGTTCTATTTCTGAAGCACAGCAATCTC
CTTTATGTTAATGTGATTGGTCTGGCTGATGATTCAGTTCGGTCTCTAAAGCTCCCTCCTTGGGCCTGT
GGATTCATAATGGATACAAATTCTGACCCAAAGAACTGCCCCTTTCAACTTTGCTCTCCAATACGTCCC
CCAAAATATTACACATACAAGTTTGCAGAAGGCAAACTGTTTGAGGAAACTGGGCATGAAGACCCAATC
ACAAAGACTAGTCGCGTTTTACGTCTAGAAGCCAAAAGCAAGGATGGAAAATTAGTGCCAATGACTGTT
TTCCACAAAACTGACTCTGAGGACTTGCAGAAGAAACCTCTCTTGGTACATGTATATGGAGCTTATGGA
ATGGATTTGAAAATGAATTTCAGGCCTGAGAGGCGGGTCCTGGTGGATGATGGATGGATATTAGCATAC
TGCCATGTTCGAGGTGGTGGTGAGTTAGGCCTCCAGTGGCACGCTGATGGCCGCCTAACTAAAAAACTC
AATGGCCTTGCTGATTTAGAGGCTTGCATTAAGACGCTTCATGGCCAAGGCTTTTCTCAGCCAAGTCTA
ACAACCCTGACTGCTTTCAGTGCTGGAGGGGTGCTTGCAGGAGCATTGTGTAATTCTAATCCAGAGCTG
GTGAGAGCGGTGACTTTGGAGGCACCTTTCTTGGATGTTCTCAACACCATGATGGACACTACACTTCCT
CTGACATTAGAAGAATTAGAAGAATGGGGGAATCCTTCATCTGATGAAAAACACAAGAACTACATAAAA
CGTTACTGTCCCTATCAAAATATTAAACCTCAGCATTATCCTTCAATTCACATAACGGCATATGAAAAC
GATGAACGGGTACCTCTGAAAGGAATTGTAAGTTATACTGAGAAACTCAAGGAAGCCATCGCGGAGCAT
GCTAAGGACACAGGTGAAGGCTATCAGACCCCTAATATTATTCTAGATATTCAGCCTGGAGGCAATCAT
GTAATTGAGGATTCTCACAAAAAGATTACAGCCCAAATTAAATTCCTGTACGAGGAACTTGGACTTGAC
AGCACCAGTGTTTTCGAGGATCTTAAGAAATACCTGAAATTCTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_006036
Insert Size 2184 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006036.4
RefSeq Size 6903 bp
RefSeq ORF 2184 bp
Locus ID 9581
UniProt ID Q4J6C6
Cytogenetics 2p21
Protein Families Druggable Genome, Protease
MW 83.9 kDa
Summary The protein encoded by this gene belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene.provided by RefSeq, Jan 2010
Transcript Variant: This variant (1, also known as variant C) represents the longest transcript, and encodes the longest isoform (1). Variants 1-3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_006036" in other vectors (4)
SKU Description Size Price
RC213731 PREPL (Myc-DDK-tagged)-Human prolyl endopeptidase-like (PREPL), transcript variant 1 10 ug
$665.00
RC213731L3 Lenti ORF clone of Human prolyl endopeptidase-like (PREPL), transcript variant 1, Myc-DDK-tagged 10 ug
$966.00
RC213731L4 Lenti ORF clone of Human prolyl endopeptidase-like (PREPL), transcript variant 1, mGFP tagged 10 ug
$966.00
RG213731 PREPL (tGFP-tagged) - Human prolyl endopeptidase-like (PREPL), transcript variant 1 10 ug
$866.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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