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KRT85 (NM_002283) Human Untagged Clone

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SKU
SC303167
KRT85 (untagged)-Human keratin 85 (KRT85)
  $520.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol KRT85
Synonyms ECTD4; Hb-5; HB5; hHb5; K85; KRTHB5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC303167 representing NM_002283.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCGTGCCGCTCCTACAGGATCAGCTCAGGATGCGGGGTCACCAGGAACTTCAGCTCCTGCTCAGCT
GTGGCCCCCAAAACTGGCAACCGCTGCTGCATCAGCGCCGCCCCCTACCGAGGGGTGTCCTGCTACCGA
GGGCTGACGGGCTTCGGCAGCCGCAGCCTCTGCAACCTGGGCTCCTGCGGGCCCCGGATAGCTGTAGGT
GGCTTCCGAGCCGGCTCCTGCGGACGCAGCTTCGGCTACCGCTCCGGGGGCGTGTGCGGACCCAGCCCC
CCATGCATCACTACCGTGTCGGTCAACGAGAGCCTCCTCACGCCCCTCAACCTGGAGATCGACCCCAAC
GCACAGTGCGTGAAGCAGGAGGAGAAGGAGCAGATCAAGTCCCTCAACAGCAGGTTCGCGGCCTTCATC
GACAAGGTGCGCTTCCTGGAGCAGCAGAACAAGCTGCTGGAGACCAAGTGGCAGTTCTACCAGAACCAG
CGCTGCTGCGAGAGCAACCTGGAGCCACTGTTCAGTGGCTACATCGAGACTCTGCGGCGGGAGGCCGAG
TGCGTGGAGGCCGACAGCGGGAGGCTGGCCTCAGAGCTCAACCATGTGCAGGAGGTGCTGGAGGGCTAC
AAGAAGAAGTATGAAGAGGAGGTGGCCCTGAGAGCCACAGCAGAGAATGAGTTTGTCGTTCTAAAGAAG
GACGTGGACTGTGCCTACCTGCGGAAATCAGACCTGGAGGCCAATGTGGAGGCCCTGGTGGAGGAGTCT
AGCTTCCTGAGGCGCCTCTATGAAGAGGAGATCCGCGTTCTCCAAGCCCACATCTCAGACACCTCGGTC
ATAGTCAAGATGGACAACAGCCGAGACCTGAACATGGACTGCATCATCGCTGAGATCAAGGCTCAGTAT
GACGATGTTGCCAGCCGCAGCCGGGCCGAGGCTGAGTCCTGGTACCGTAGCAAGTGTGAGGAGATGAAG
GCCACGGTGATCAGGCATGGGGAGACCCTGCGCCGCACCAAGGAGGAGATCAACGAGCTGAACCGCATG
ATCCAGAGGCTGACGGCCGAGATTGAGAATGCCAAGTGCCAGCGTGCCAAGCTGGAGGCTGCTGTGGCT
GAGGCAGAGCAGCAGGGTGAGGCGGCCCTCAGCGATGCCCGCTGCAAGCTGGCTGAGCTGGAGGGCGCC
CTGCAGAAGGCCAAGCAGGACATGGCCTGCCTGCTCAAGGAGTACCAGGAGGTGATGAACTCCAAGCTG
GGCCTGGACATCGAGATCGCCACCTACAGGCGCCTGCTGGAGGGCGAGGAACACAGGCTGTGTGAAGGT
GTGGGCTCTGTGAATGTCTGTGTCAGCAGCTCCCGTGGTGGAGTCTCCTGTGGGGGCCTCTCCTACAGC
ACCACCCCAGGGCGCCAGATCACTTCTGGCCCCTCAGCCATAGGCGGCAGCATCACGGTGGTGGCCCCT
GACTCCTGTGCCCCCTGCCAGCCTCGTTCCTCCAGCTTCAGCTGCGGGAGTAGCCGGTCGGTCCGCTTT
GCCTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_002283
Insert Size 1524 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002283.3
RefSeq Size 2491 bp
RefSeq ORF 1524 bp
Locus ID 3891
UniProt ID P78386
Cytogenetics 12q13.13
MW 55.8 kDa
Summary The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
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"NM_002283" in other vectors (4)
SKU Description Size Price
RC220351 KRT85 (Myc-DDK-tagged)-Human keratin 85 (KRT85) 10 ug
$289.00 $472.00
RC220351L3 Lenti ORF clone of Human keratin 85 (KRT85), Myc-DDK-tagged 10 ug
$772.00
RC220351L4 Lenti ORF clone of Human keratin 85 (KRT85), mGFP tagged 10 ug
$772.00
RG220351 KRT85 (tGFP-tagged) - Human keratin 85 (KRT85) 10 ug
$672.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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