DUSP28 (NM_001033575) Human Untagged Clone

SKU
SC302742
DUSP28 (untagged)-Human dual specificity phosphatase 28 (DUSP28)
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DUSP28
Synonyms DUSP26; VHP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC302742 representing NM_001033575.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGACCGGCAGAAGCTGGGCGCCGCGGGGCCGCCTCGCCCGTACCTCCACCGTTGGTGCGCGTCGCG
CCCTCACTCTTCCTCGGGAGCGCGCGAGCCGCGGGCGCGGAGGAGCAGCTGGCGCGCGCGGGAGTCACG
CTGTGCGTCAACGTCTCCCGCCAGCAGCCCGGCCCGCGCGCGCCCGGCGTGGCAGAGCTGCGCGTGCCC
GTGTTCGACGACCCGGCTGAGGACCTGCTGGCGCACCTGGAGCCCACGTGCGCCGCCATGGAGGCCGCG
GTGCGCGCCGGCGGCGCCTGCCTAGTCTACTGCAAGAACGGCCGCAGCCGCTCGGCCGCCGTCTGCACC
GCGTACCTCATGCGGCACCGCGGCCTCAGCCTGGCGAAGGCCTTCCAGATGGTGAAGAGCGCTCGCCCG
GTAGCAGAACCGAACCCGGGCTTCTGGTCTCAGCTCCAGAAGTATGAGGAGGCCCTCCAGGCCCAGTCC
TGCCTGCAGGGAGAGCCCCCAGCCTTAGGGTTGGGCCCTGAGGCTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001033575
Insert Size 531 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033575.1
RefSeq Size 1555 bp
RefSeq ORF 531 bp
Locus ID 285193
UniProt ID Q4G0W2
Cytogenetics 2q37.3
MW 18.3 kDa
Summary Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate (in vitro) (PubMed:24531476, PubMed:29121083). Has almost no detectable activity with phosphotyrosine, even less activity with phosphothreonine and displays complete lack of activity with phosphoserine (PubMed:29121083). The poor activity with phosphotyrosine may be due to steric hindrance by bulky amino acid sidechains that obstruct access to the active site (PubMed:29121083).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033575" in other vectors (4)
SKU Description Size Price
RC206912 DUSP28 (Myc-DDK-tagged)-Human dual specificity phosphatase 28 (DUSP28) 10 ug
$300.00
RC206912L3 Lenti ORF clone of Human dual specificity phosphatase 28 (DUSP28), Myc-DDK-tagged 10 ug
$600.00
RC206912L4 Lenti ORF clone of Human dual specificity phosphatase 28 (DUSP28), mGFP tagged 10 ug
$600.00
RG206912 DUSP28 (tGFP-tagged) - Human dual specificity phosphatase 28 (DUSP28) 10 ug
$500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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