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PHF12 (NM_001033561) Human Untagged Clone

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SKU
SC302730
PHF12 (untagged)-Human PHD finger protein 12 (PHF12), transcript variant 1
  $875.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PHF12
Synonyms PF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC302730 representing NM_001033561.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTGGGAGAAAATGGAGACCAAGACGATCGTGTACGACTTGGACACATCAGGGGGGCTGATGGAGCAA
ATCCAAGCTCTGCTGGCTCCCCCCAAGACGGACGAGGCAGAAAAGCGCAGTCGGAAGCCTGAGAAGGAG
CCCCGGAGAAGCGGCAGGGCCACCAACCACGACAGCTGCGATAGCTGCAAGGAAGGTGGAGATCTCCTG
TGCTGCGACCACTGCCCGGCTGCCTTCCACCTCCAGTGCTGTAACCCTCCACTGAGTGAAGAAATGTTG
CCTCCTGGAGAGTGGATGTGTCACCGGTGCACTGTTCGCCGAAAGAAACGAGAGCAGAAAAAGGAGCTG
GGTCATGTCAATGGACTGGTGGACAAATCTGGCAAACGGACTACATCCCCCAGCAGTGACACTGACTTG
TTGGACAGATCGGCCAGCAAAACTGAACTAAAGGCCATTGCCCATGCCCGGATCCTGGAAAGGAGAGCC
AGCAGGCCTGGCACACCCACATCCAGCGCCAGCACAGAGACTCCCACCTCTGAGCAGAATGATGTCGAC
GAAGACATCATTGACGTGGATGAGGAACCAGTAGCAGCGGAGCCAGACTATGTGCAGCCCCAGCTGAGG
CGGCCCTTTGAGCTGCTGATTGCTGCCGCCATGGAGCGGAACCCCACCCAATTTCAGTTGCCCAATGAA
CTGACTTGTACCACTGCACTACCAGGTTCTAGCAAGAGGAGAAGAAAGGAGGAAACCACAGGGAAAAAT
GTTAAGAAGACACAGCATGAATTAGATCACAATGGTCTCGTTCCCTTACCCGTCAAAGTCTGCTTCACG
TGTAACAGGAGTTGCCGTGTGGCTCCTCTCATCCAGTGTGACTATTGCCCTCTCCTGTTTCACATGGAT
TGCCTCGAGCCGCCGCTCACTGCCATGCCCCTGGGCAGATGGATGTGTCCGAATCACATCGAACATGTG
GTGCTGAACCAGAAGAATATGACACTGAGCAATCGGTGCCAGGTGTTTGATCGTTTCCAGGACACCGTT
TCGCAGCATGTCGTCAAAGTGGACTTCCTGAACCGAATCCACAAGAAGCACCCCCCTAACCGGCGTGTG
CTCCAGTCGGTCAAAAGAAGAAGCTTGAAGGTTCCTGATGCTATAAAATCTCAGTACCAGTTTCCACCC
CCTCTCATTGCACCCGCGGCCATTCGGGACGGGGAGCTGATCTGCAATGGGATCCCTGAGGAATCACAG
ATGCACCTTTTGAACTCTGAGCACTTAGCCACCCAAGCAGAGCAGCAAGAGTGGCTCTGTAGTGTTGTT
GCGCTCCAGTGCAGCATATTGAAACATTTATCTGCTAAGCAGATGCCTTCGCATTGGGACTCTGAACAG
ACAGAGAAGGCTGATATTAAGCCTGTTATTGTGACTGACAGCTCAGTCACCACCTCCCTGCAAACAGCT
GACAAGACACCTACACCTTCCCACTACCCCTTGTCCTGCCCCTCAGGGATTAGCACCCAGAATTCCCTG
AGCTGCTCTCCACCCCACCAGTCCCCAGCCCTAGAGGACATCGGCTGCAGTTCTTGTGCGGAAAAATCC
AAGAAAACCCCTTGTGGGACTGCCAATGGGCCAGTGAACACAGAGGTGAAAGCTAATGGCCCACACCTC
TACAGCAGCCCTACTGATTCCACGGACCCCCGGCGACTTCCAGGCGCTAACACCCCACTACCAGGCCTC
TCACACCGGCAAGGCTGGCCCCGGCCCCTCACGCCACCAGCGGCTGGGGGCCTTCAGAACCACACCGTC
GGCATCATTGTGAAGACAGAGAATGCCACTGGCCCCAGCTCTTGCCCCCAGAGGAGTTTGGTTCCTGTC
CCAAGCCTGCCCCCTTCCATTCCCAGCTCTTGTGCCAGCATCGAGAACACCAGCACTTTGCAAAGAAAG
ACTGTCCAATCACAGATAGGACCTCCGTTGACAGATTCAAGGCCACTGGGCTCACCCCCAAACGCCACC
CGGGTGCTCACTCCCCCGCAAGCAGCAGGAGATGGTATCTTGGCCACAACAGCCAACCAACGATTCAGC
TCACCAGCGCCATCGTCAGATGGCAAGGTCAGCCCCGGCACGTTATCCATAGGAAGCGCTTTAACCGTA
CCCTCTTTCCCAGCCAACTCTACTGCCATGGTGGACCTCACCAACTCACTTCGAGCATTTATGGATGTC
AATGGAGAAATCGAGATAAATATGCTGGACGAGAAGCTGATCAAGTTTCTGGCCTTGCAGAGAATACAT
CAGCTTTTCCCCTCCCGGGTCCAACCTTCACCGGGCAGTGTCGGGACACATCAGCTGGCTTCTGGAGGG
CACCACATAGAAGTGCAAAGAAAGGAGGTACAGGCCCGAGCTGTGTTCTACCCCCTCTTAGGGTTGGGA
GGAGCTGTGAACATGTGCTATCGAACCCTCTACATCGGGACAGGAGCTGACATGGATGTGTGCCTTACA
AACTATGGTCACTGTAACTACGTGTCCGGGAAACATGCCTGCATATTCTACGATGAGAATACCAAACAT
TATGAGCTGTTAAACTACAGTGAGCATGGGACAACGGTGGACAATGTGCTGTATTCATGTGACTTCTCG
GAGAAGACCCCGCCAACCCCCCCAAGCAGTATTGTTGCCAAAGTGCAGAGTGTCATCAGGCGCCGCCGG
CACCAGAAACAGGACGAAGAGCCAAGTGAGGAGGCAGCCATGATGAGTTCCCAGGCCCAGGGGCCGCAG
CGGAGACCCTGCAATTGCAAAGCCAGCAGCTCGAGCTTGATTGGGGGCAGTGGGGCCGGCTGGGAGGGC
ACAGCCTTACTGCACCATGGCAGCTACATCAAGCTGGGCTGCCTGCAGTTTGTCTTCAGCATCACTGAG
TTTGCGACCAAACAGCCCAAAGGCGATGCCAGCCTGCTGCAGGATGGGGTCTTGGCCGAGAAGCTCTCT
CTCAAGCCCCACCAGGGCCCTGTGCTGCGCTCCAACTCTGTTCCTTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001033561
Insert Size 3015 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033561.1
RefSeq Size 4475 bp
RefSeq ORF 3015 bp
Locus ID 57649
UniProt ID Q96QT6
Cytogenetics 17q11.2
Protein Families Druggable Genome, Transcription Factors
MW 109.7 kDa
Summary Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional TLE5 complexes to DNA.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_001033561" in other vectors (4)
SKU Description Size Price
RC216199 PHF12 (Myc-DDK-tagged)-Human PHD finger protein 12 (PHF12), transcript variant 1 10 ug
$874.00
RC216199L3 Lenti ORF clone of Human PHD finger protein 12 (PHF12), transcript variant 1, Myc-DDK-tagged 10 ug
$1,174.00
RC216199L4 Lenti ORF clone of Human PHD finger protein 12 (PHF12), transcript variant 1, mGFP tagged 10 ug
$1,174.00
RG216199 PHF12 (tGFP-tagged) - Human PHD finger protein 12 (PHF12), transcript variant 1 10 ug
$1,074.00

file-alt Citations

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