ATP2B2 (NM_001001331) Human Untagged Clone

SKU SC300165

ATP2B2 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ATP2B2
• Synonyms: PMCA2; PMCA2a; PMCA2i
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC300165 representing NM_001001331.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAG; TTCGGGTGCACAATGGAGGAGCTCCGCTCCCTCATGGAGCTGCGGGGCACTGAGGCTGTGGTCAAGATC; AAGGAGACTTATGGGGACACCGAAGCCATCTGCCGGCGCCTCAAAACCTCACCTGTTGAAGGTTTGCCG; GGCACCGCTCCAGACCTGGAAAAGAGAAAGCAAATTTTTGGGCAAAACTTTATACCTCCAAAGAAGCCA; AAAACCTTCCTGCAGCTCGTGTGGGAGGCGCTGCAGGACGTGACGCTCATCATCCTGGAGATTGCCGCC; ATCATCTCCCTGGGGCTGTCCTTCTACCACCCGCCCGGCGAGGGCAACGAAGGATGTGCGACGGCCCAG; GGTGGGGCAGAGGATGAAGGAGAGGCAGAGGCAGGTTGGATCGAGGGGGCCGCCATTCTCCTCTCAGTT; ATCTGTGTGGTCCTGGTCACGGCCTTCAATGACTGGAGCAAAGAGAAACAGTTCCGGGGCCTGCAGAGC; CGCATCGAGCAGGAACAGAAATTTACCGTGGTCCGGGCTGGCCAGGTGGTCCAGATCCCTGTGGCTGAG; ATCGTGGTTGGGGACATAGCCCAGGTCAAATATGGTGACCTCCTCCCTGCCGACGGCCTCTTCATCCAG; GGCAATGACCTCAAGATTGATGAAAGCTCCCTAACTGGAGAGTCTGACCAGGTGCGCAAGTCCGTGGAC; AAGGACCCCATGCTGCTGTCAGGAACCCACGTGATGGAGGGCTCAGGACGGATGTTGGTGACTGCTGTG; GGTGTGAACTCTCAGACTGGCATCATCTTTACCCTCCTGGGGGCTGGTGGTGAAGAGGAAGAGAAGAAA; GACAAAAAAGGTGTGAAGAAGGGGGATGGCCTTCAGCTACCAGCAGCAGACGGTGCGGCAGCTTCAAAT; GCTGCAGATAGTGCGAATGCCAGCCTAGTCAATGGTAAAATGCAGGATGGCAATGTGGACGCCAGCCAG; AGCAAAGCCAAACAACAGGACGGGGCAGCCGCCATGGAGATGCAGCCCCTCAAGAGTGCCGAGGGCGGC; GACGCTGACGACAGGAAGAAGGCCAGCATGCACAAGAAGGAGAAGTCCGTGCTGCAGGGCAAGCTCACC; AAGCTGGCTGTGCAGATCGGGAAGGCGGGCTTGGTGATGTCAGCCATCACGGTGATCATCCTGGTGCTC; TACTTCACTGTGGACACCTTCGTGGTCAACAAGAAGCCGTGGCTGCCTGAGTGCACGCCCGTCTACGTG; CAGTACTTTGTCAAGTTCTTCATCATTGGCGTGACGGTGCTGGTGGTCGCCGTGCCCGAGGGGCTCCCT; CTGGCCGTCACCATCTCGTTGGCCTATTCGGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCAC; CTGGATGCCTGTGAGACCATGGGCAATGCCACAGCCATCTGCTCAGACAAGACAGGCACGCTGACCACC; AATCGCATGACAGTGGTACAGGCCTATGTCGGCGACGTCCACTATAAAGAGATCCCCGACCCCAGCTCC; ATCAACACCAAGACCATGGAGCTGCTGATCAATGCCATCGCCATCAACAGCGCCTACACCACCAAGATT; CTGCCCCCAGAGAAGGAGGGCGCCCTGCCTCGGCAGGTGGGCAACAAGACGGAGTGCGGCCTGCTGGGC; TTCGTGCTGGACCTGAAGCAGGACTACGAGCCCGTGCGCAGCCAGATGCCAGAGGAGAAGTTGTACAAA; GTGTACACCTTCAACTCCGTGCGCAAGTCCATGAGCACTGTCATCAAGCTGCCCGACGAGAGCTTCCGC; ATGTACAGCAAGGGGGCTTCTGAGATCGTGCTCAAGAAGTGCTGCAAAATCCTCAATGGGGCGGGAGAG; CCTCGTGTCTTCCGGCCCCGCGACCGGGACGAGATGGTAAAGAAGGTGATTGAGCCCATGGCTTGCGAT; GGGCTCCGCACTATCTGCGTGGCCTACCGCGACTTCCCCAGCAGCCCGGAGCCGGACTGGGACAATGAG; AATGACATCCTCAACGAACTCACCTGCATCTGCGTGGTGGGCATCGAGGACCCGGTGCGGCCAGAGGTC; CCAGAAGCCATCCGCAAGTGCCAGCGGGCAGGCATCACGGTCCGCATGGTCACTGGCGACAATATCAAC; ACGGCTCGGGCCATCGCCATCAAGTGTGGCATCATCCATCCTGGGGAGGACTTTCTGTGCCTCGAGGGC; AAGGAGTTCAACAGGAGGATCCGCAACGAGAAGGGGGAGATTGAGCAGGAGCGAATTGACAAGATCTGG; CCAAAGCTGCGGGTGCTGGCTCGCTCCTCCCCAACGGACAAGCATACCCTGGTTAAAGGCATCATCGAC; AGCACACACACTGAGCAGCGGCAGGTGGTGGCCGTGACGGGGGACGGGACCAACGACGGGCCTGCACTC; AAGAAGGCCGACGTGGGCTTCGCCATGGGCATCGCAGGCACTGACGTGGCCAAGGAGGCCTCAGACATC; ATCCTGACAGACGACAATTTCAGCAGCATCGTCAAGGCAGTGATGTGGGGCCGCAACGTCTATGACAGC; ATCTCCAAATTCTTGCAGTTCCAGCTCACCGTCAACGTGGTGGCCGTGATTGTGGCCTTCACAGGCGCC; TGCATCACGCAGGACTCCCCTCTGAAGGCCGTGCAGATGCTCTGGGTGAACCTCATCATGGACACGTTT; GCCTCGCTGGCACTGGCCACTGAGCCGCCCACGGAGACCCTGCTGCTGAGGAAGCCGTACGGCCGCAAC; AAGCCGCTCATCTCCAGGACCATGATGAAGAACATCCTGGGCCATGCTGTCTACCAGCTTGCCCTCATC; TTCACCCTGCTCTTTGTTGGCGAGAAGATGTTCCAGATCGACAGCGGGAGGAACGCGCCCCTGCATTCG; CCACCCTCAGAACATTACACCATCATCTTCAACACCTTCGTCATGATGCAGCTCTTCAACGAGATCAAC; GCCCGCAAGATCCACGGCGAGCGCAATGTCTTTGACGGCATCTTCCGGAACCCCATCTTCTGCACCATC; GTGCTGGGCACCTTTGCCATCCAGATAGTGATCGTGCAGTTTGGAGGGAAGCCATTCAGCTGCTCTCCA; CTGCAGCTGGACCAGTGGATGTGGTGCATATTCATTGGGTTAGGAGAGCTCGTTTGGGGCCAGGTCATC; GCCACCATCCCGACCAGCAGACTCAAGTTCCTCAAGGAGGCAGGCAGGCTCACACAGAAGGAGGAGATC; CCGGAGGAGGAGCTCAACGAGGACGTGGAGGAGATCGACCACGCGGAGCGGGAGCTGCGGCGGGGCCAG; ATCCTGTGGTTCCGAGGCCTGAATCGGATCCAGACACAGATCCGCGTCGTGAAGGCGTTCCGTAGCTCT; CTCTATGAAGGTTTAGAAAAGCCTGAATCTCGAACCTCCATCCATAACTTCATGGCTCATCCTGAATTC; CGGATCGAAGATTCCCAGCCCCACATCCCCCTCATTGATGACACCGACCTGGAAGAAGATGCCGCGCTC; AAGCAGAACTCGAGCCCGCCGTCATCCCTCAACAAGAACAACAGCGCCATCGACAGTGGGATCAACCTG; ACGACCGACACAAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCCATCCACAGCCTGGAGACGTCG; CTTTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001331
• Insert Size: 3732 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001001331.2
• RefSeq Size: 8962 bp
• RefSeq ORF: 3732 bp
• Locus ID: 491
• UniProt ID: Q01814
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• MW: 136.9 kDa
• Summary: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longest isoform (1), also known as WB. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.