ATP2B2 (NM_001001331) Human Tagged ORF Clone

SKU RC221710

ATP2B2 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ATP2B2
• Synonyms: PMCA2; PMCA2a; PMCA2i
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221710 representing NM_001001331; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT; TCGGGTGCACAATGGAGGAGCTCCGCTCCCTCATGGAGCTGCGGGGCACTGAGGCTGTGGTCAAGATCAA; GGAGACTTATGGGGACACCGAAGCCATCTGCCGGCGCCTCAAAACCTCACCTGTTGAAGGTTTGCCGGGC; ACCGCTCCAGACCTGGAAAAGAGAAAGCAAATTTTTGGGCAAAACTTTATACCTCCAAAGAAGCCAAAAA; CCTTCCTGCAGCTCGTGTGGGAGGCGCTGCAGGACGTGACGCTCATCATCCTGGAGATTGCCGCCATCAT; CTCCCTGGGGCTGTCCTTCTACCACCCGCCCGGCGAGGGCAACGAAGGATGTGCGACGGCCCAGGGTGGG; GCAGAGGATGAAGGAGAGGCAGAGGCAGGTTGGATCGAGGGGGCCGCCATTCTCCTCTCAGTTATCTGTG; TGGTCCTGGTCACGGCCTTCAATGACTGGAGCAAAGAGAAACAGTTCCGGGGCCTGCAGAGCCGCATCGA; GCAGGAACAGAAATTTACCGTGGTCCGGGCTGGCCAGGTGGTCCAGATCCCTGTGGCTGAGATCGTGGTT; GGGGACATAGCCCAGGTCAAATATGGTGACCTCCTCCCTGCCGACGGCCTCTTCATCCAGGGCAATGACC; TCAAGATTGATGAAAGCTCCCTAACTGGAGAGTCTGACCAGGTGCGCAAGTCCGTGGACAAGGACCCCAT; GCTGCTGTCAGGAACCCACGTGATGGAGGGCTCAGGACGGATGTTGGTGACTGCTGTGGGTGTGAACTCT; CAGACTGGCATCATCTTTACCCTCCTGGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGGTG; TGAAGAAGGGGGATGGCCTTCAGCTACCAGCAGCAGACGGTGCGGCAGCTTCAAATGCTGCAGATAGTGC; GAATGCCAGCCTAGTCAATGGTAAAATGCAGGATGGCAATGTGGACGCCAGCCAGAGCAAAGCCAAACAA; CAGGACGGGGCAGCCGCCATGGAGATGCAGCCCCTCAAGAGTGCCGAGGGCGGCGACGCTGACGACAGGA; AGAAGGCCAGCATGCACAAGAAGGAGAAGTCCGTGCTGCAGGGCAAGCTCACCAAGCTGGCTGTGCAGAT; CGGGAAGGCGGGCTTGGTGATGTCAGCCATCACGGTGATCATCCTGGTGCTCTACTTCACTGTGGACACC; TTCGTGGTCAACAAGAAGCCGTGGCTGCCTGAGTGCACGCCCGTCTACGTGCAGTACTTTGTCAAGTTCT; TCATCATTGGCGTGACGGTGCTGGTGGTCGCCGTGCCCGAGGGGCTCCCTCTGGCCGTCACCATCTCGTT; GGCCTATTCGGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGAGACCATG; GGCAATGCCACAGCCATCTGCTCAGACAAGACAGGCACGCTGACCACCAATCGCATGACAGTGGTACAGG; CCTATGTCGGCGACGTCCACTATAAAGAGATCCCCGACCCCAGCTCCATCAACACCAAGACCATGGAGCT; GCTGATCAATGCCATCGCCATCAACAGCGCCTACACCACCAAGATTCTGCCCCCAGAGAAGGAGGGCGCC; CTGCCTCGGCAGGTGGGCAACAAGACGGAGTGCGGCCTGCTGGGCTTCGTGCTGGACCTGAAGCAGGACT; ACGAGCCCGTGCGCAGCCAGATGCCAGAGGAGAAGTTGTACAAAGTGTACACCTTCAACTCCGTGCGCAA; GTCCATGAGCACTGTCATCAAGCTGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGGGCTTCTGAGATC; GTGCTCAAGAAGTGCTGCAAAATCCTCAATGGGGCGGGAGAGCCTCGTGTCTTCCGGCCCCGCGACCGGG; ACGAGATGGTAAAGAAGGTGATTGAGCCCATGGCTTGCGATGGGCTCCGCACTATCTGCGTGGCCTACCG; CGACTTCCCCAGCAGCCCGGAGCCGGACTGGGACAATGAGAATGACATCCTCAACGAACTCACCTGCATC; TGCGTGGTGGGCATCGAGGACCCGGTGCGGCCAGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCGGGCAG; GCATCACGGTCCGCATGGTCACTGGCGACAATATCAACACGGCTCGGGCCATCGCCATCAAGTGTGGCAT; CATCCATCCTGGGGAGGACTTTCTGTGCCTCGAGGGCAAGGAGTTCAACAGGAGGATCCGCAACGAGAAG; GGGGAGATTGAGCAGGAGCGAATTGACAAGATCTGGCCAAAGCTGCGGGTGCTGGCTCGCTCCTCCCCAA; CGGACAAGCATACCCTGGTTAAAGGCATCATCGACAGCACACACACTGAGCAGCGGCAGGTGGTGGCCGT; GACGGGGGACGGGACCAACGACGGGCCTGCACTCAAGAAGGCCGACGTGGGCTTCGCCATGGGCATCGCA; GGCACTGACGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGACGACAATTTCAGCAGCATCGTCAAGG; CAGTGATGTGGGGCCGCAACGTCTATGACAGCATCTCCAAATTCTTGCAGTTCCAGCTCACCGTCAACGT; GGTGGCCGTGATTGTGGCCTTCACAGGCGCCTGCATCACGCAGGACTCCCCTCTGAAGGCCGTGCAGATG; CTCTGGGTGAACCTCATCATGGACACGTTTGCCTCGCTGGCACTGGCCACTGAGCCGCCCACGGAGACCC; TGCTGCTGAGGAAGCCGTACGGCCGCAACAAGCCGCTCATCTCCAGGACCATGATGAAGAACATCCTGGG; CCATGCTGTCTACCAGCTTGCCCTCATCTTCACCCTGCTCTTTGTTGGCGAGAAGATGTTCCAGATCGAC; AGCGGGAGGAACGCGCCCCTGCATTCGCCACCCTCAGAACATTACACCATCATCTTCAACACCTTCGTCA; TGATGCAGCTCTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAATGTCTTTGACGGCATCTT; CCGGAACCCCATCTTCTGCACCATCGTGCTGGGCACCTTTGCCATCCAGATAGTGATCGTGCAGTTTGGA; GGGAAGCCATTCAGCTGCTCTCCACTGCAGCTGGACCAGTGGATGTGGTGCATATTCATTGGGTTAGGAG; AGCTCGTTTGGGGCCAGGTCATCGCCACCATCCCGACCAGCAGACTCAAGTTCCTCAAGGAGGCAGGCAG; GCTCACACAGAAGGAGGAGATCCCGGAGGAGGAGCTCAACGAGGACGTGGAGGAGATCGACCACGCGGAG; CGGGAGCTGCGGCGGGGCCAGATCCTGTGGTTCCGAGGCCTGAATCGGATCCAGACACAGATCCGCGTCG; TGAAGGCGTTCCGTAGCTCTCTCTATGAAGGTTTAGAAAAGCCTGAATCTCGAACCTCCATCCATAACTT; CATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATTGATGACACCGACCTG; GAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCATCCCTCAACAAGAACAACAGCGCCATCG; ACAGTGGGATCAACCTGACGACCGACACAAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCCATCCA; CAGCCTGGAGACGTCGCTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221710 representing NM_001001331; Red=Cloning site Green=Tags(s); ; MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPG; TAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGEGNEGCATAQGG; AEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVV; GDIAQVKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMLVTAVGVNS; QTGIIFTLLGAGGEEEEKKDKKGVKKGDGLQLPAADGAAASNAADSANASLVNGKMQDGNVDASQSKAKQ; QDGAAAMEMQPLKSAEGGDADDRKKASMHKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDT; FVVNKKPWLPECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETM; GNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINTKTMELLINAIAINSAYTTKILPPEKEGA; LPRQVGNKTECGLLGFVLDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEI; VLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCI; CVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEK; GEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIA; GTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQM; LWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNILGHAVYQLALIFTLLFVGEKMFQID; SGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDGIFRNPIFCTIVLGTFAIQIVIVQFG; GKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEAGRLTQKEEIPEEELNEDVEEIDHAE; RELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIHNFMAHPEFRIEDSQPHIPLIDDTDL; EEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001331
• ORF Size: 3729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001001331.4
• RefSeq Size: 6821 bp
• RefSeq ORF: 3732 bp
• Locus ID: 491
• UniProt ID: Q01814
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• MW: 136.7 kDa
• Summary: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]