GNPDA2 (NM_138335) Human Untagged Clone

SKU SC128186

GNPDA2 (untagged)-Human glucosamine-6-phosphate deaminase 2 (GNPDA2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GNPDA2
• Synonyms: GNP2; SB52
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128186 sequence for NM_138335 edited (data generated by NextGen Sequencing); ATGAGGCTTGTAATTCTTGATAACTATGACTTGGCTAGTGAATGGGCAGCCAAATACATC; TGTAATCGCATCATTCAGTTCAAACCTGGACAGGACAGATATTTTACACTGGGTTTACCA; ACAGGGAGTACACCTTTAGGATGCTATAAAAAACTAATAGAATATCATAAGAATGGACAC; CTTTCTTTTAAATATGTGAAGACCTTTAATATGGATGAATATGTAGGACTTCCAAGAAAT; CATCCTGAAAGCTACCATTCTTATATGTGGAATAATTTTTTTAAGCATATCGATATAGAT; CCTAATAATGCACATATCCTTGACGGGAATGCTGCAGATTTACAAGCAGAATGTGATGCT; TTTGAAAACAAAATAAAAGAAGCTGGAGGAATAGATCTTTTTGTTGGAGGAATTGGTCCA; GATGGTCATATCGCTTTCAATGAGCCTGGATCCAGTTTAGTGTCAAGGACAAGATTAAAG; ACTCTAGCAATGGATACCATCTTGGCAAATGCCAAATATTTTGATGGAGATTTATCAAAA; GTGCCAACTATGGCTCTAACTGTTGGTGTGGGGACAGTGATGGATGCTAGAGAAGTAATG; ATCCTTATAACAGGGGCACACAAGGCATTTGCCCTGTACAAAGCAATAGAAGAAGGAGTC; AATCACATGTGGACTGTTTCCGCTTTCCAGCAGCATCCCCGGACTATTTTTGTATGCGAT; GAAGATGCTACTTTAGAATTAAGAGTTAAAACTGTGAAATACTTTAAAGGTCTAATGCAT; GTGCACAATAAACTTGTGGATCCACTATTCAGTATGAAAGATGGAAACTGA; ; Clone variation with respect to NM_138335.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138335 unedited; TGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGCTGTTCCAGCGTCGAAGAA; CCATTGGGTCTGCCGGTTTGAACTTGTTCTGGAAGCTGTGCGTCACCGTAATGAGGCTTG; TAATTCTTGATAACTATGACTTGGCTAGTGAATGGGCAGCCAAATACATCTGTAATCGCA; TCATTCAGTTCAAACCTGGACAGGACAGATATTTTACACTGGGTTTACCAACAGGGAGTA; CACCTTTAGGATGCTATAAAAAACTAATAGAATATCATAAGAATGGACACCTTTCTTTTA; AATATGTGAAGACCTTTAATATGGATGAATATGTAGGACTTCCAAGAAATCATCCTGAAA; GCTACCATTCTTATATGTGGAATAATTTTTTTAAGCATATCGATATAGATCCTAATAATG; CACATATCCTTGACGGGAATGCTGCAGATTTACAAGCAGAATGTGATGCTTTTGAAAACA; AAATAAAAGAAGCTGGAGGAATAGATCTTTTTGTTGGAGGAATTGGTCCAGATGGTCATA; TCGCTTTCAATGAGCCTGGATCCAGTTTAGTGTCAAGGACAAGATTAAAGACTCTAGCAA; TGGATACCATCTTGGCAAATGCCAAATATTTTGATGGAGATTTATCAAAAGTGCCAACTA; TGGCTCTAACTGGTTTGGTGTGGGGACAGTNGATGGATGCTANNAGAGTNNATGATCCTT; ATAACAGGGGCACACAAGGCATNNTGCCCTGTACAAAGCANTAGAAGAAAGGAGTCNATC; ACATGTGGACTGGTTTCCGCTTTCCAGCAGCATCCCCGGACTATTTTGTATGCGATGAAG; ATGCTACTTTAAATTTAAGAGTTAAACTGGTGAATACTTTAAGGTCTAAGGCATGTGCAC; ATAACTCTGGGGATCCATCTTTTCAGTTGGAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_138335 unedited; AGCTATGNNACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTTCATT; AATTTATTTGCTATTTTAATATTTTAAGAAACTACATTTTAATCACATAGACAAGCAGAT; AAGTAAGGCTTCTTCTACCAGGTGGGCTTATGAAATGCAAAATAAGCAAAAGGAGCATTT; TTTTGCTCCCCACAGAGGCAAAGACATCTTTTAACCTAACTCTCCTAATGCATGATGGTA; ACAAACCCAAAATGAATGCAGGTTCACATGTACTATAATTGTTGGGAGTCGAATGCATGT; ATTCTTCAGGTTCACTTCTGGAAATTTAGATAACTTATTTATTACACGCTTTATTTGAGT; TAAAGATAAAAAACAATATACTTTTATGTAAATTGCTATGGCAATGTAGTTTACAGTAAT; TCCTTTTATATATACTTCGTATTATTCTTTTAGACATTTTATAAGGAATAAAGCTAATTG; TATATTAAGTTTAACAATAATCAGAAAATATTCTCCAAAAGAATTTCCTGAAGTGGCTTG; CTAGGTATAAAGCTCATAATTGTCAAGATACTTATATTAGGGACATGAACCCAAGTCGTT; AAATAAAAATAAGATATTTGTAAAAAAGTGCTATAGAAGAAGGTGCACAAAANACATGCA; CTTACACATACTTTCCAAAAAGATGTAAACTGTACTTTTAGGCACTGTCATCTCTTCAAA; TTCCTTTACCAATGATATATAGCACAATTTTTGATGTCTAGATGGTGCTGTAGGAAGATG; ACTAAGGCAACCACGTGCAGAAAAGCATGTGTGTGATGCACAGAAAATAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_138335
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138335.1, NP_612208.1
• RefSeq Size: 1939 bp
• RefSeq ORF: 831 bp
• Locus ID: 132789
• UniProt ID: Q8TDQ7
• Cytogenetics: 4p12
• Domains: Glucosamine_iso
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways
• Summary: The protein encoded by this gene is an allosteric enzyme that catalyzes the reversible reaction converting D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Variations of this gene have been reported to be associated with influencing body mass index and susceptibility to obesity. A pseudogene of this gene is located on chromosome 9. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).