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ERK5 (MAPK7) (NM_139032) Human Untagged Clone

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SKU
SC127852
MAPK7 (untagged)-Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 2
  $621.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ERK5
Synonyms BMK1; ERK4; ERK5; PRKM7
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC127852 sequence for NM_139032 edited (data generated by NextGen Sequencing)
ATGGAAAGCGACCTGCACCAGATCATCCACTCCTCACAGCCCCTCACACTGGAACACGTG
CGCTACTTCCTGTACCAACTGCTGCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATC
CACCGTGACCTGAAGCCCTCCAACCTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGT
GACTTTGGTATGGCTCGTGGCCTGTGCACCTCGCCCGCTGAACATCAGTACTTCATGACT
GAGTATGTGGCCACGCGCTGGTACCGTGCGCCCGAGCTCATGCTCTCTTTGCATGAGTAT
ACACAGGCTATTGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGCCCGGCGC
CAGCTCTTCCCAGGCAAAAACTATGTACACCAGCTACAGCTCATCATGATGGTGCTGGGT
ACCCCATCACCAGCCGTGATTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATCCAG
AGCTTGCCACCACGCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGGTGCCGACCGCCAG
GCCCTATCACTGCTGGGTCGCATGCTGCGTTTTGAGCCCAGCGCTCGCATCTCAGCAGCT
GCTGCCCTTCGCCACCCTTTCCTGGCCAAGTACCATGATCCTGATGATGAGCCTGACTGT
GCCCCGCCCTTTGACTTTGCCTTTGACCGCGAAGCCCTCACTCGGGAGCGCATTAAGGAG
GCCATTGTGGCTGAAATTGAGGACTTCCATGCAAGGCGTGAGGGCATCCGCCAACAGATC
CGCTTCCAGCCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCCAGATGTTGAAATG
CCCAGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGGAGTCTCCACCACCAGCCCCGCCA
CCATGCCCCGGCCCTGCACCTGACACCATTGATCTGACCCTGCAGCCACCTCCACCAGTC
AGTGAGCCTGCCCCACCAAAGAAAGATGGTGCCATCTCAGACAATACTAAGGCTGCCCTT
AAAGCTGCCCTGCTCAAGTCTTTGAGGAGCCGGCTCAGAGATGGCCCCAGCGCACCCCTG
GAGGCTCCTGAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCCAGCGGGAGCGGGAGGAG
AAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGGAGAAACGGCGGCAGGAGCGGGAG
CGAAAGGAACGGGGGGCTGGGGCCTCTGGGGGCCCCTCCACTGACCCCTTGGCTGGACTA
GTGCTCAGTGACAATGACAGAAGCCTGTTGGAACGCTGGACTCGAATGGCCCGGCCCGCA
GCCCCAGCCCTCACCTCTGTGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACCCCA
GTCCAACCTACCAGTCCTCCTCCTGGCCCTGTAGCCCAGCCCACTGGCCCGCAACCACAA
TCTGCGGGCTCTACCTCTGGCCCTGTACCCCAGCCTGCCTGCCCACCCCCTGGCCCTGCA
CCCCACCCCACTGGCCCTCCTGGGCCCATCCCTGTCCCCGCGCCACCCCAGATTGCCACC
TCCACCAGCCTCCTGGCTGCCCAGTCACTTGTGCCACCCCCTGGGCTGCCTGGCTCCAGC
ACCCCAGGAGTTTTGCCTTACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGAGCC
CCTCAGTCTTCCATGTCAGAGTCACCTGATGTCAACCTTGTGACCCAGCAGCTATCTAAG
TCACAGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAGGCACACCAAAGGGCAGTGGGGCT
GGCTACGGTGTTGGCTTTGACCTGGAGGAATTCTTAAACCAGTCTTTCGACATGGGCGTG
GCTGATGGGCCACAGGATGGCCAGGCAGATTCAGCCTCTCTCTCAGCCTCCCTGCTTGCT
GACTGGCTCGAAGGCCATGGCATGAACCCTGCCGATATTGAGTCCCTGCAGCGTGAGATC
CAGATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCCCTGA

Clone variation with respect to NM_139032.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_139032 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCGCGAGGCGCGGGCTCCGCAGAGAGAAG
CAGAGGGTTGGGCGGCCGCCTCGGTTAACTCCGCTGCAGCCCAAAGGACGGGAATCGCGG
GACAGACAAACGAGCGGAGGGAAGATACCTAGAAGCCAGGAAACCGCGAGCTGCAGTCCA
ACTTGGCCGGAAGCTGCGGAGAGGCTCAGCCACCGGAAGTCAGTGGAGGGTTCGGCCGGA
CGCTCTAGAATCCCGGAGGACCGGGATCTCTGTGGTTGGCCGTGACGGGCACCCTCTACC
GGGGATGACACATTCCCAGAGCTCCTGGGACCAAGCAAATGGCGGACACAATTCCCTGGG
CGGAAGGGGACTTCGGGAGCCAGTAGCCAAGCTACGTGGTCCTGGACCTGATGGAAAGCG
ACCTGCACCAGATCATCCACTCCTCACAGCCCCTCACACTGGAACACGTGCGCTACTTCC
TGTACCAACTGCTGCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTGACC
TGAAGCCCTCCAACCTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGTGACTTTGGTA
TGGCTCGTGGCCTGTGCACCTCGCCCGCTGAACATCAGTACTTCATGACTGAGTATGTGG
CCACGCGCTGGTACCGTGCGCCCGAGCTCATGCTCTCTTTGCATGAGTATACACAGGCTA
TTGACCTCTGGTCTGG
Restriction Sites Please inquire |
ACCN NM_139032
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139032.1, NP_620601.1
RefSeq Size 2737 bp
RefSeq ORF 2034 bp
Locus ID 5598
UniProt ID Q13164
Cytogenetics 17p11.2
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Gap junction, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway
Summary The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) lacks a segment in the 5' region, which includes the translation start codon, when compared to variant 1. The translation of this transcript begins at a downstream in-frame start codon, and thus results in an N-terminal truncated isoform (2), as compared to isoform 1.
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Documents
Resources
"NM_139032" in other vectors (6)
SKU Description Size Price
RC200655 MAPK7 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 2 10 ug
$620.00
RC200655L1 Lenti ORF clone of Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 2, Myc-DDK-tagged 10 ug
$920.00
RC200655L2 Lenti ORF clone of Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 2, mGFP tagged 10 ug
$920.00
RC200655L3 Lenti ORF clone of Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 2, Myc-DDK-tagged 10 ug
$920.00
RC200655L4 Lenti ORF clone of Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 2, mGFP tagged 10 ug
$920.00
RG200655 MAPK7 (tGFP-tagged) - Human mitogen-activated protein kinase 7 (MAPK7), transcript variant 2 10 ug
$789.00 $820.00

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