MSRB2 (NM_012228) Human Untagged Clone

SKU SC127636

MSRB2 (untagged)-Human methionine sulfoxide reductase B2 (MSRB2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MSRB2
• Synonyms: CBS-1; CBS1; CGI-131; MSRB; PILB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127636 sequence for NM_012228 edited (data generated by NextGen Sequencing); ATGGCGCGGCTCCTCTGGTTGCTCCGGGGCCTGACCCTCGGAACTGCGCCTCGGCGGGCG; GTGCGGGGCCAAGCGGGCGGCGGCGGGCCCGGCACCGGGCCGGGACTGGGGGAGGCAGGG; TCTCTTGCAACGTGTGAGCTGCCTCTTGCCAAGAGTGAGTGGCAAAAGAAACTAACCCCG; GAGCAGTTCTACGTCACAAGAGAAAAGGGAACGGAACCGCCTTTCAGTGGGATCTACCTG; AATAACAAGGAAGCAGGAATGTATCATTGCGTGTGCTGCGACAGTCCACTCTTCAGTTCT; GAGAAAAAGTACTGCTCTGGCACTGGGTGGCCTTCGTTTTCCGAGGCTCATGGTACGTCT; GGCTCTGATGAAAGCCACACAGGGATCCTGAGACGTCTGGATACCTCGTTAGGATCAGCT; CGCACAGAGGTTGTCTGCAAGCAGTGTGAAGCTCATCTAGGTCACGTGTTTCCTGATGGA; CCTGGGCCCAATGGTCAGAGGTTTTGCATCAACAGTGTGGCTTTGAAGTTCAAACCAAGG; AAACACTGA; ; Clone variation with respect to NM_012228.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012228 unedited; TTCAAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTGGGAT; GGGAGCAGGGGCAGAGACGGGCAGAGGGCAGAGGGCGGAGCGGCGCCGGAGCGGGCGTCA; TGGCGCGGCTCCTCTGGTTGCTCCGGGGCCTGACCCTCGGAACTGCGCCTCGGCGGGCGG; TGCGGGGCCAAGCGGGCGGCGGCGGGCCCGGCACCGGGCCGGGACTGGGGGAGGCAGGGT; CTCTTGCAACGTGTGAGCTGCCTCTTGCCAAGAGTGAGTGGCAAAAGAAACTAACCCCGG; AGCAGTTCTACGTCACAAGAGAAAAGGGAACGGAACCGCCTTTCAGTGGGATCTACCTGA; ATAACAAGGAAGCAGGAATGTATCATTGCGTGTGCTGCGACAGTCCACTCTTCAGTTCTG; AGAAAAAGTACTGCTCTGGCACTGGGTGGCCTTCGTTTTCCGAGGCTCATGGTACGTCTG; GCTCTGATGAAAGCCACACAGGGATCCTGAGACGTCTGGATACCTCGTTAGGATCAGCTC; GCACAGAGGTTGTCTGCAAGCAGTGTGAAGCTCATCTAGGTCACGTGTTTCCTGATGGAC; CTGGGCCCAATGGTCAGAGGTTTTGCATCAACAGTGTGGCTTTGAAGTTCAAACCAAGGA; AACACTGACCATCTTCAAGAGTCCCGTTCCCTTGCCACCCCTTCACGTGCACCCTCAATT; TCCACAATTCACTTGAATGACTTGTTTTATTTGCATAAAACTGGGCTGAATTTGAAAAAA; AAAAAAAAACTCGCTCTAGATTGCGCCGCGTCATAGCTGTTCCTGAACGATCCCGGTGGC; ATCCTGTGACCCCTCCCATGCCTTCCTGCCCTGGAGTGCCATNCATGCCCACANCCTGTC; TATAAATAAGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012228 unedited; CGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTCAAATTCAGCCCATTTTT; ATTGCAAATAAAACAAGTCATTCAAGTGAATTGTGGAAATTGAGGGTGCACGTGAAGGGG; TGGCAAGGGAACGGGACTCTTGAAGATGGTCAGTGTTTCCTTGGTTTGAACTTCAAAGCC; ACACTGTTGATGCAAAACCTCTGACCATTGGGCCCAGGTCCATCAGGAAACACGTGACCT; ATATGAGCTTCACACTGCTTGCAGACAACCTCTGTGCGAGCTGATCCTAACGAGGTATCC; AGACGTCTCAGGATCCCTGTGTGGCTTTCATCAGAGCCAGACGTACCATGAGCCTCGGAA; AACGAAGGCCACCCAGTGCCAGAGCAGTACTTTTTCTCAGAACTGAAGAGTGGACTGTCG; CAGCACACGCAATGATACATTCCTGCTTCCTTGTTATTCAGGTAGATCCCACTGAAAGGC; GGTTCCGTTCCCTTTTCTCTTGTGACGTAAAACTGCTCCGGGGTTAGTTTCTTTTGCCAC; TCACTCTTGGCAAGAGGCAGCTCACACGTTGCAAGAGACCCTGCCTCCCCCAGTCCCGGC; CCGGTGCCGGGCCCGCCGCCGCCCGCTTGGCCCCGTACCGCCCGCCGAGGCGCATTTCCG; AGGGTCAGGCCCCGTAGCAACCAGAGGAGCCGCGCCATGACGCCCGCTCCGGNGCCGTTC; ACGCCTCTGCCCTCTGCCGTCTCTGCCCCTGCTNCCATCCACCCTCGTGCCGAATTCGCG; GCCGCCCTATAGTGAGTCGTATTACAAAATCTGACGTTCACTANACGACCTCTGCTTTTT; AGACCTNCCACCGTAACGCCTACCGCCATTTGCGTAACGGGCGGGTTTTTACACCTTTGG; AAAGCCCGTGTTTTGTGGCAAACAACTCCATGGAGGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_012228
• Insert Size: 770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012228.2, NP_036360.2
• RefSeq Size: 903 bp
• RefSeq ORF: 606 bp
• Locus ID: 22921
• UniProt ID: Q9Y3D2
• Cytogenetics: 10p12.2
• Domains: SelR
• Protein Families: Transcription Factors
• Summary: Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Upon oxidative stress, may play a role in the preservation of mitochondrial integrity by decreasing the intracellular reactive oxygen species build-up through its scavenging role, hence contributing to cell survival and protein maintenance.[UniProtKB/Swiss-Prot Function]