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GPR55 (NM_005683) Human Untagged Clone

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SKU
SC126570
GPR55 (untagged)-Human G protein-coupled receptor 55 (GPR55)
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GPR55
Synonyms LPIR1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC126570 sequence for NM_005683 edited (data generated by NextGen Sequencing)
ATGAGTCAGCAAAACACCAGTGGGGACTGCCTGTTTGACGGTGTCAACGAGCTGATGAAA
ACCCTACAGTTTGCAGTCCACATCCCCACCTTCGTCCTGGGCCTGCTCCTCAACCTGCTG
GCCATCCATGGCTTCAGCACCTTCCTTAAGAACAGGTGGCCCGATTATGCTGCCACCTCC
ATCTACATGATCAACCTGGCAGTCTTTGACCTGCTGCTGGTGCTCTCCCTCCCATTCAAG
ATGGTCCTGTCCCAGGTACAGTCCCCCTTCCCGTCCCTGTGCACCCTGGTGGAGTGCCTT
TACTTCGTCAGCATGTACGGAAGCGTCTTCACCATCTGCTTCATCAGCATGGACCGGTTC
TTGGCCATCTGTTACCCGCTACTGGTGAGCCACCTCCGGTCCCCCAGGAAGATCTTTGGG
ATCTGCTGCACCATCTGGGTCCTGGTGTGGACCGGAAGCATCCCTATCTACAGTTTCCAT
GGGAAAGTGGAAAAATACATGTGCTTCCACAACATGTCTGATGATACCTGGAGCGCCAAG
GTCTTCTTCCCGCTGGAGGTGTTTGGCTTCCTCCTTCCCATGGGCATCATGGGCTTCTGC
TGCTCCAGGAGCATCCACATCCTGCTGGGCCGCCGAGACCACACCCAGGACTGGGTGCAG
CAGAAAGCCTGCATCTACAGCATCGCAGCCAGCCTGGCTGTCTTCGTGGTCTCCTTCCTC
CCAGTCCACCTGGGGTTCTTCCTGCAGTTCCTGGTGAGAAACAGCTTTATCGTAGAGTGC
AGAGCCAAGCAGAGCATCAGCTTCTTCTTGCAATTGTCCATGTGTTTCTCCAACGTCAAC
TGCTGCCTGGATGTTTTCTGCTACTACTTTGTCATCAAAGAATTCCGCATGAACATCAGG
GCCCACCGGCCTTCCAGGGTCCAGCTGGTCCTGCAGGACACCACGATCTCCCGGGGCTAA

Clone variation with respect to NM_005683.3
370 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005683 unedited
NGTGAAGTCGGATTTGTATACACTCACTATAGGCGGCCGCAATCTANAGTCGAGTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
CCCAGGCCCAGCAGGTTCTATTTTTGTCAAAAAAAAATGTGTGTTTGGTAAAAAGAGAGT
GAAAAACCGATTCTGGAATGATTTCCCCAAAAAGTACCAGGGGTTGCCTCTGGGCCCCAG
GATAAAAGGTAATTTTTCTTTTCGGGGTTCTAGTTGGGATTCAAGGGGCATAAATTGCTG
TGTGATCCTGGGGAGGTGTTTGTGATTCTTGACAAAGTCTTTTGAACCCCTCCCTTCAAA
AAAGTGAAAGGGCCCCCCTCTGATCTGTTGGGGTTGTAGGAAAAAACATGATTCACCAAA
ACCCCCGTGGGGACTGCCTGTTTGACGGGGTCAACAACCTGATGAAAACCCTACAGTTTG
GAGTCCAAATCCCCCCCTTTGTCCGGGGCCTGCTCCTAAACCTGGTGGCCATCCAGGGTT
TCAGCCCCTTCCTTAAAAACAGGGGGCCCGATTATGCTGCCCCCTCCATCTACATGATCA
ACCTGGCAGTCTTTGACCGGGGGCGGGGGCTCCCCCCCCCATTCAAAAGGGCCCTGTCCC
AGGTACAGTCCCCCTTCCCGTCCCGGGGCACCCTGGGGGAGGGCCTTTACTTCGTCAGCA
TGTACGGAAGCGTCTTCCCCATCTGTTTAATCAGCATGGACCGGTTCTTGGCCATCTGTT
ACCCGCTACTGGTGAGCCACCTCCGGTCCCCCAGGAAGATCTTTGGGATCCGCTGCACCT
CCGGGTCCTGTGGTGGACCGGAACCTCCCTTCAACGTTTA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005683 unedited
AGNAAAGCACTGGGGNAGGGTCACAGGGCATGCCACCCATCCGTTACCAGTAATTCAGGC
CAGGGCTTTCTTCCCCTGAACAGGATGTCCTTCCGTTAGCCCCGGGAGATCGTGGTGTCC
TGCAGGACCAGCTGGACCCTGGAAGGCCGGTGGGCCCTGATGTTCATGCGGAATTCTTTG
ATGACAAAGTAGTAGCAGAAAACATCCAGGCAGCAGTTGACGTTGGAGAAACACATGGAC
AATTGCAAGAAGAAGCTGATGCTCTGCTTGGCTCTGCACTCTACGATAAAGCTGTTTCTC
ACCAGGAACTGCAGGAAGAACCCCAGGTGGACTGGGAGGAAGGAGACCACGAAGACAGCC
AGGCTGGCTGCGATGCTGTAGATGCAGGCTTTCTGCTGCACCCAGTCCTGGGTGTGGTCT
CGGCGGCCCAGCAGGATGTGGATGCTCCTGGAGCAGCAGAAGCCCATGATGCCCATGGGA
AGGAGGAAGCCAAACACCTCCAGCGGGAAGAAGACCTTGGCGCTCCAGGTATCATCAGAC
ATGTTGTGGAAGCACATGTATTTTTCCACTTTCCCATGGAAACTGTAGATAGGGATGCTT
CCGGTCCACACCAGGACCCAGATGGTGCAGCAGATCCCAAAGATCTTCCTGGGGGACCGG
AGGTGGCTCACCAGTAGCGGGTAACAGATGGCCAAGAACCGGTCCATGCTGATGAAGCAG
ATGGTGAAGACGCTTCCGTACATGCTGACGAAGTANAGGCACTCCACCAGGGTGCACAGG
GACGGGAAGGGGGACTGTACCTGGGACAGGACCATCTTTGATGGGAGGGAGAGCACCAGC
AGCAGGTCAAAGACTGCCAGGTTGATCATGTAGATG
Restriction Sites Please inquire |
ACCN NM_005683
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005683.2, NP_005674.2
RefSeq Size 2629 bp
RefSeq ORF 960 bp
Locus ID 9290
UniProt ID Q9Y2T6
Cytogenetics 2q37.1
Protein Families Druggable Genome, GPCR, Transmembrane
Summary This gene belongs to the G-protein-coupled receptor superfamily. The encoded integral membrane protein is a likely cannabinoid receptor. It may be involved in several physiological and pathological processes by activating a variety of signal transduction pathways. provided by RefSeq, Aug 2013
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Documents
Resources
"NM_005683" in other vectors (6)
SKU Description Size Price
RC207570 GPR55 (Myc-DDK-tagged)-Human G protein-coupled receptor 55 (GPR55) 10 ug
$300.00
RC207570L1 Lenti ORF clone of Human G protein-coupled receptor 55 (GPR55), Myc-DDK-tagged 10 ug
$600.00
RC207570L2 Lenti ORF clone of Human G protein-coupled receptor 55 (GPR55), mGFP tagged 10 ug
$600.00
RC207570L3 Lenti ORF clone of Human G protein-coupled receptor 55 (GPR55), Myc-DDK-tagged 10 ug
$600.00
RC207570L4 Lenti ORF clone of Human G protein-coupled receptor 55 (GPR55), mGFP tagged 10 ug
$600.00
RG207570 GPR55 (tGFP-tagged) - Human G protein-coupled receptor 55 (GPR55) 10 ug
$489.00 $500.00

file-alt Citations

The GPR55 ligand L-alpha-lysophosphatidylinositol promotes RhoA-dependent Ca2+ signaling and NFAT activation
Henstridge, Balenga, Ford et al
FASEB J (2009) 23 (1), 183-93 DOI: 10.1096/fj.08-108670
Cited by 290
AI Summary: OriGene products contributed to this study by providing the recombinant human GPR55 cDNA (OriGene, SC119563), which was used to engineer the HEK293 cell line for investigating LPI-induced signaling pathways.
The GPR55 ligand L-alpha-lysophosphatidylinositol promotes RhoA-dependent Ca2+ signaling and NFAT activation
Henstridge, Balenga, Ford et al
FASEB J (2009) 23 (1), 183-93 DOI: 10.1096/fj.08-108670
Cited by 290
AI Summary: In this paper, OriGene’s human GPR55 cDNA (OriGene, SC116509) was used to generate HEK293 cells stably expressing GPR55, enabling the authors to investigate LPI-induced signaling pathways.
Showing 1-2 of 2 papers.
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