SPINLW1 (EPPIN) (NM_020398) Human Untagged Clone

SKU SC126420

EPPIN (untagged)-Human serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) (SPINLW1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SPINLW1
• Synonyms: CT71; CT72; dJ461P17.2; SPINLW1; WAP7; WFDC7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020398 edited; CACCAGAAAGGCTGGCCTCTCTTCAACATGGGATCTTCTGGACTTTTGAGCCTCCTGGTG; CTATTCGTCCTCTTAGCGAATGTCCAGGGACCTGGTCTGACTGATTGGTTATTTCCCAGG; AGATGTCCCAAAATCAGAGAAGAATGTGAATTCCAAGAAAGGGATGTGTGTACAAAGGAC; AGACAATGCCAGGACAACAAGAAGTGTTGTGTCTTCAGCTGCGGAAAAAAATGTTTAGAT; CTCAAACAAGATGTATGCGAAATGCCAAAAGAAACTGGCCCCTGCCTGGCTTATTTTCTT; CATTGGTGGTATGACAAGAAAGATAATACTTGCTCCATGTTTGTCTATGGTGGCTGCCAG; GGAAACAATAACAACTTCCAATCCAAAGCCAACTGCCTGAACACCTGCAAGAATAAACGC; TTTCCCTGATTGGATAAGGATGCACTGGAAGAACTGCCAGAATGTGGCTCATGCTCTGAG; TACTGTTCCTGTACCTGACTGATGCTCCAGACTGGCTTCCAGTTTCACTCTCAGCATTCC; AAGATCTTAGCCCTTCCCAGAACAGAACGCTTGCATCTACCTCCTCTTCCTCCATCTTTG; GCTCTTTTGATGCACAATATCCATCCGTTTTGATTTCATCTTTATGTCCCCTTTATCTCC; AACTTCTAGAACTCCCAGTTTATACCTGTGTCACTCTCAATTTTTTCCAGTAAAGTACTT; GATGTAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020398 unedited; NGGTCAAAATTTGTATACGACTCATATAGGCGGCCGCGNATTCAGATCTGGTACCGGTCC; GGATTCCCGGGATCCCAGAAGGCTGGCCTCTCTTCAACATGGGATCTTCTGGACTTTTGA; GCCTCCTGGTGCTATTCGTCCTCTTAGCGAATGTCCAGGGACCTGGTCTGACTGATTGGT; TATTTCCCAGGAGATGTCCCAAAATCAGAGAAGAATGTGAATTCCAAGAAAGGGATGTGT; GTACAAAGGACAGACAATGCCAGGACAACAAGAAGTGTTGTGTCTTCAGCTGCGGAAAAA; AATGTTTAGATCTCAAACAAGATGTATGCGAAATGCCAAAAGAAACTGGCCCCTGCCTGG; CTTATTTTCTTCATTGGTGGTATGACAAGAAAGATAATACTTGCTCCATGTTTGTCTATG; GTGGCTGCCAGGGAAACAATAACAACTTCCAATCCAAAGCCAACTGCCTGAACACCTGCA; AGAATAAACGCTTTCCCTGATTGGATAAGGATGCACTGGAAGAACTGCCAGAATGTGGCT; CATGCTCTGAGTACTGTTCCTGTACCTGACTGATGCTCCAGACTGGCTTCCAGTTTCACT; CTCAGCATTCCAAGATCTTAGCCCTTCCCAGAACAGAACGCTTGCATCTACCTCCTCTTC; CTCCATCTTTGGCTCTTTTGATGCACAATATCCATCCGTTTTGATTTCATCTTTATGTCC; CCTTTATCTCCAACTTCTAGAACTCCCAGTTTATACCTGTGTCACTCTCAATTTTTTCAG; TANAGTACTTGATGTNNNANAAAANNNNNANANNNAAAAAGGCGGCCGCGGTCATAGCTG; TTCCTNGACAGATCCCGGTGTGCATCCCTGTGACCCCTCCCCAGTGCCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_020398
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020398.2, NP_065131.1
• RefSeq Size: 1961 bp
• RefSeq ORF: 402 bp
• Locus ID: 57119
• UniProt ID: O95925
• Cytogenetics: 20q13.12
• Protein Families: Secreted Protein
• Summary: This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility and exhibits antimicrobial activity against E. coli, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]; Transcript Variant: This variant (1) encodes the longer isoform (1), which is also known as eppin-1 and eppin-3.