GDF 5 (GDF5) (NM_000557) Human Untagged Clone

CAT#: SC125956

GDF5 (untagged)-Human growth differentiation factor 5 (GDF5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GDF 5
• Synonyms: BDA1C; BMP-14; BMP14; CDMP1; DUPANS; LAP-4; LAP4; OS5; SYM1B; SYNS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000557 edited
  CGCTGCCACTGGTTAGCGATAATACTGGCAGCGCGACCGGTTTCTGAGGGTTGTTCACCT
  TGCTGCTCAGACGCTCCGAGAAGCTTCAGGCAGCATAACACTTACAGTAGAGACAGGGTT
  TCACCATGTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCCACCTGCCTCGGCCT
  CCCAAGGTGCTGGGATTGCAGGCGTGAGCCACTGCGCCTGGCCGCAATTTACTTCATTGA
  ATCTCCAACAAGAGTCCTGTGAGCTGCTGACTGGAGACGGTGCACGTCTGGATACGAGAG
  CATTTCCACTATGGGACTGGATACAAACACACACCCGGCAGACTTCAAGAGTCTCAGACT
  GAGGAGAAAGCCTTTCCTTCTGCTGCTACTGCTGCTGCCGCTGCTTTTGAAAGTCCACTC
  CTTTCATGGTTTTTCCTGCCAAACCAGAGGCACCTTTGCTGCTGCCGCTGTTCTCTTTGG
  TGTCATTCAGCGGCTGGCCAGAGGATGAGACTCCCCAAACTCCTCACTTTCTTGCTTTGG
  TACCTGGCTTGGCTGGACCTGGAATTCATCTGCACTGTGTTGGGTGCCCCTGACTTGGGC
  CAGAGACCCCAGGGGACCAGGCCAGGATTGGCCAAAGCAGAGGCCAAGGAGAGGCCCCCC
  CTGGCCCGGAACGTCTTCAGGCCAGGGGGTCACAGCTATGGTGGGGGGGCCACCAATGCC
  AATGCCAGGGCAAAGGGAGGCACCGGGCAGACAGGAGGCCTGACACAGCCCAAGAAGGAT
  GAACCCAAAAAGCTGCCCCCCAGACCGGGCGGCCCTGAACCCAAGCCAGGACACCCTCCC
  CAAACAAGGCAGGCTACAGCCCGGACTGTGACCCCAAAAGGACAGCTTCCCGGAGGCAAG
  GCACCCCCAAAAGCAGGATCTGTCCCCAGCTCCTTCCTGCTGAAGAAGGCCAGGGAGCCC
  GGGCCCCCACGAGAGCCCAAGGAGCCGTTTCGCCCACCCCCCATCACACCCCACGAGTAC
  ATGCTCTCGCTGTACAGGACGCTGTCCGATGCTGACAGAAAGGGAGGCAACAGCAGCGTG
  AAGTTGGAGGCTGGCCTGGCCAACACCATCACCAGCTTTATTGACAAAGGGCAAGATGAC
  CGAGGTCCCGTGGTCAGGAAGCAGAGGTACGTGTTTGACATTAGTGCCCTGGAGAAGGAT
  GGGCTGCTGGGGGCCGAGCTGCGGATCTTGCGGAAGAAGCCCTCGGACACGGCCAAGCCA
  GCGGCCCCCGGAGGCGGGCGGGCTGCCCAGCTGAAGCTGTCCAGCTGCCCCAGCGGCCGG
  CAGCCGGCCTCCTTGCTGGATGTGCGCTCCGTGCCAGGCCTGGACGGATCTGGCTGGGAG
  GTGTTCGACATCTGGAAGCTCTTCCGAAACTTTAAGAACTCGGCCCAGCTGTGCCTGGAG
  CTGGAGGCCTGGGAACGGGGCAGGGCCGTGGACCTCCGTGGCCTGGGCTTCGACCGCGCC
  GCCCGGCAGGTCCACGAGAAGGCCCTGTTCCTGGTGTTTGGCCGCACCAAGAAACGGGAC
  CTGTTCTTTAATGAGATTAAGGCCCGCTCTGGCCAGGACGATAAGACCGTGTATGAGTAC
  CTGTTCAGCCAGCGGCGAAAACGGCGGGCCCCACTGGCCACTCGCCAGGGCAAGCGACCC
  AGCAAGAACCTTAAGGCTCGCTGCAGTCGGAAGGCACTGCATGTCAACTTCAAGGACATG
  GGCTGGGACGACTGGATCATCGCACCCCTTGAGTACGAGGCTTTCCACTGCGAGGGGCTG
  TGCGAGTTCCCATTGCGCTCCCACCTGGAGCCCACGAATCATGCAGTCATCCAGACCCTG
  ATGAACTCCATGGACCCCGAGTCCACACCACCCACCTGCTGTGTTCCCACGCGGCTGAGT
  CCCATCAGCATCCTCTTCATTGACTCTGCCAACAACGTGGTGTATAAGCAGTATGAGGAC
  ATGGTCGTGGAGTCGTGTGGCTGCAGGTAGCAGCACTGGCCCTCTGTCTTCCTGGGTGGC
  ACATCCCAAGAGCCCCTTCCTGCACTCCTGGAATCACAGAGGGGTCAGGAAGCTGTGGCA
  GGAGCATCTACACAGCTTGGGTGAAAGGGGATTCCAATAAGCTTGCTCGCTCTCTGAGTG
  TGACTTGGGCTAAAGGCCCCCTTTTATCCACAAGTTCCCCTGGCTGAGGATTGCTGCCCG
  TCTGCTGATGTGACCAGTGGCAGGCACAGGTCCAGGGAGACAGACTCTGAATGGGACTGA
  GTCCCAGGAAACAGTGCTTTCCGATGAGACTCAGCCCACCATTTCTCCTCACCTGGGCCT
  TCTCAGCCTCTGGACTCTCCTAAGCACCTCTCAGGAGAGCCACAGGTGCCACTGCCTCCT
  CAAATCACATTTGTGCCTGGTGACTTCCTGTCCCTGGGACAGTTGAGAAGCTGACTGGGC
  AAGAGTGGGAGAGAAGAGGAGAGGGCTTGGATAGAGTTGAGGAGTGTGAGGCTGTTAGAC
  TGTTAGATTTAAATGTATATTGATGAGATAAAAAGCAAAACTGTGCCTAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000557 unedited
  CATATTTGTATACGACTCACTATAGGCGGCCGCGTAATCAGATCTGGTACCGGTCCGGAA
  TTCCCGGGATCGCTGCCACTGGTTAGCGATAATACTGGCAGCGCGACCGGTTTCTGAGGG
  TTGTTCACCTTGCTGCTCAGACGCTCCGAGAAGCTTCAGGCAGCATAACACTTACAGTAG
  AGACAGGGTTTCACCATGTTAGCCAGGATGGTCTTGATCTCCTGACCTCGTGATCCACCT
  GCCTCGGCCTCCCAAGGTGCTGGGATTGCAGGCGTGAGCCACTGCGCCTGGCCGCAATTT
  ACTTCATTGAATCTCCAACAAGAGTCCTGTGAGCTGCTGACTGGAGACGGTGCACGTCTG
  GATACGAGAGCATTTCCACTATGGGACTGGATACAAACACACACCCGGCAGACTTCAAGA
  GTCTCAGACTGAGGAGAAAGCCTTTCCTTCTGCTGCTACTGCTGCTGCCGCTGCTTTTGA
  AAGTCCACTCCTTTCATGGTTTTTCCTGCCAAACCAGAGGCACCTTTGCTGCTGCCGCTG
  TTCTCTTTGGTGTCATTCAGCGGCTGGCCAGAGGATGAGACTCCCCAAACTCCTCACTTT
  CTTGCTTTGGTACCTGGCTTGGCTGGACCTGGAATTCATCTGCACTGTGTTTGGGTGCCC
  CTGATTTGGGCCAAAGACCCCAGGGGACAGGCCAGGATTGGCCCAAACAGAGGCCAAGGA
  GAGGCCCCCCTGGGCCCGGAACGTCATCAGGCCGGGGGGTCAAACCTGGGGGGGGGGGCC
  ACCAAGCCAATGCCGGGGAAAGGGGGACCCGGGACAAACAGGGGCCTGACACCCCCAAAA
  GAGAGAACCAACAAGAGGCGCCCAAAAAGGGAGGGCCCGAAACCCAGCAGGAACCCCTCC
  CCTAACAAGGCGGGTACAAGCAAAGGAGAGGACCCAAAAGGAAGCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_000557
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000557.2, NP_000548.1
• RefSeq Size: 2383 bp
• RefSeq ORF: 1506 bp
• Locus ID: 8200
• UniProt ID: P43026
• Cytogenetics: 20q11.22
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway
• Protein Pathways: Cytokine-cytokine receptor interaction, TGF-beta signaling pathway
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mutations in this gene are associated with acromesomelic dysplasia, brachydactyly, chondrodysplasia, multiple synostoses syndrome, proximal symphalangism, and susceptibility to osteoarthritis. [provided by RefSeq, Aug 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.