PRKACA (NM_002730) Human Untagged Clone
SKU
SC125607
PRKACA (untagged)-Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | PRKACA |
| Synonyms | CAFD1; PKACA; PPNAD4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_002730 |
| Insert Size | 1800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002730.3, NP_002721.1 |
| RefSeq Size | 2689 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 5566 |
| UniProt ID | P17612 |
| Cytogenetics | 19p13.12 |
| Domains | pkinase, S_TKc, S_TK_X, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway |
| Summary | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. provided by RefSeq, Jan 2015 Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002730" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210332 | PRKACA (Myc-DDK-tagged)-Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1 | 10 ug |
$457.00
|
|
| RC210332L1 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC210332L2 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC210332L3 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC210332L4 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RG210332 | PRKACA (tGFP-tagged) - Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1 | 10 ug |
$489.00
$657.00
|
|
Citations
Prostaglandin (PG)D(2) and 15-deoxy-Delta(12,14)-PGJ(2), but not PGE(2), mediate shear-induced chondrocyte apoptosis via protein kinase A-dependent regulation of polo-like kinases
Zhu, Wang, Kontrogianni-Konstantopoulos et al
Cell Death Differ (2010) 17 (8), 1325-34 DOI: 10.1038/cdd.2010.13
Zhu, Wang, Kontrogianni-Konstantopoulos et al
Cell Death Differ (2010) 17 (8), 1325-34 DOI: 10.1038/cdd.2010.13
Cited by 37
AI Summary: In this study, OriGene’s L-PGDS shRNA (SR300498) was used to knock down L-PGD synthase, demonstrating its essential role in mediating shear-induced chondrocyte apoptosis.
Protein kinase A phosphorylation activates Vpr-induced cell cycle arrest during human immunodeficiency virus type 1 infection
Barnitz, Wan, Tripuraneni et al
J Virol (2010) 84 (13), 6410-24 DOI: 10.1128/JVI.02273-09
Barnitz, Wan, Tripuraneni et al
J Virol (2010) 84 (13), 6410-24 DOI: 10.1128/JVI.02273-09
AI Summary: OriGene products, specifically the PKA catalytic subunit α cDNA (RC200149) and the Vpr cDNA clone (SC119284), were used in this study to express and analyze the roles of PKA and Vpr in elucidating the mechanism by which PKA phosphorylation activates Vpr-induced cell cycle arrest during HIV-1 infection.
Protein kinase A phosphorylation activates Vpr-induced cell cycle arrest during human immunodeficiency virus type 1 infection
Barnitz, Wan, Tripuraneni et al
J Virol (2010) 84 (13), 6410-24 DOI: 10.1128/JVI.02273-09
Barnitz, Wan, Tripuraneni et al
J Virol (2010) 84 (13), 6410-24 DOI: 10.1128/JVI.02273-09
AI Summary: OriGene products, specifically the pCMV6-XL5-Vpr plasmid, were used in this study to express the HIV-1 Vpr protein and investigate its phosphorylation and role in cell cycle arrest.
Prostaglandin (PG)D2 and 15-deoxy-?12,14-PGJ2, but not PGE2, mediate shear-induced chondrocyte apoptosis via protein kinase A-dependent regulation of polo-like kinases
Zhu, Wang, Kontrogianni-Konstantopoulos et al
Cell Death and Differentiation (2010)
Zhu, Wang, Kontrogianni-Konstantopoulos et al
Cell Death and Differentiation (2010)
AI Summary: In the referenced paper, OriGene products—including the PKD2 (Cat# SR304960) and PLK1 (Cat# SR307305) siRNA—were used to specifically knock down gene expression in chondrocytes, enabling the authors to investigate the roles of these genes in prostaglandin-mediated, shear-induced apoptosis.
Showing 1-4 of 4 papers.
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