PRKACA (NM_002730) Human Tagged ORF Clone
SKU
RC210332
PRKACA (Myc-DDK-tagged)-Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PRKACA |
| Synonyms | CAFD1; PKACA; PPNAD4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002730 |
| ORF Size | 1053 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002730.4 |
| RefSeq Size | 2689 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 5566 |
| UniProt ID | P17612, A0A024R7J0 |
| Cytogenetics | 19p13.12 |
| Domains | pkinase, S_TKc, S_TK_X, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway |
| MW | 40.4 kDa |
| Summary | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. provided by RefSeq, Jan 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002730" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210332L1 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC210332L2 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RC210332L3 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC210332L4 | Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
|
| RG210332 | PRKACA (tGFP-tagged) - Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1 | 10 ug |
$489.00
$657.00
|
|
| SC125607 | PRKACA (untagged)-Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
Modulation of huntingtin S421 phosphorylation in a Huntington's disease mouse model and its detection in nonhuman primate cerebrospinal fluid
Verani, Martufi, Petricca et al
bioRxiv (2025)
Verani, Martufi, Petricca et al
bioRxiv (2025)
AI Summary: OriGene contributed to this study by providing the pCMV6 expression plasmids for the C-term Myc-FLAG-tagged dopamine D2 receptor (D2R; catalog #RC213065) and Protein kinase A catalytic subunit (PRKACA; catalog #RC210332), which were used to investigate the phosphorylation of huntingtin at S421 in cellular assays.
Snippet: The pCMV6 expression plasmids containing the cDNA coding for C-term Myc-FLAG-tagged dopamine D2 receptor (D2R;catalog #RC213065) and Protein kinase A catalytic subunit (PKA Cα) (PRKACA;catalog #RC210332) were obtained from ORIGENE (Rockville, Maryland, United States)
PFKM phosphorylates histone H3 and promotes mitotic progression by sensing the levels of citrate
Lin, Qi, Chu et al
Nat Commun (2025) 16 (1), 6736 DOI: 10.1038/s41467-025-62111-3
Lin, Qi, Chu et al
Nat Commun (2025) 16 (1), 6736 DOI: 10.1038/s41467-025-62111-3
Cited by 7
AI Summary: OriGene products contributed pCMV6 expression plasmids encoding C-term Myc-FLAG-tagged dopamine D2 receptor (D2R; RC213065) and Protein kinase A catalytic subunit (PRKACA; RC210332), which were used for protein expression studies in the investigation of PFKM’s role in mitotic progression.
Snippet: The pCMV6 expression plasmids containing the cDNA coding for C-term Myc-FLAG-tagged dopamine D2 receptor (D2R;catalog #RC213065) and Protein kinase A catalytic subunit (PKA Cα) (PRKACA;catalog #RC210332) were obtained from ORIGENE (Rockville, Maryland, United States)
Ultrasensitive multiplexed detection of cancer biomarker proteins using SPR imaging
Ozkaya, Durak, Sharafeldin et al
Microchemical Journal (2025) 216, 114562-114562 DOI: 10.1016/j.microc.2025.114562
Ozkaya, Durak, Sharafeldin et al
Microchemical Journal (2025) 216, 114562-114562 DOI: 10.1016/j.microc.2025.114562
Cited by 5
AI Summary: In this study, OriGene's pCMV6 expression plasmids encoding Myc-FLAG-tagged dopamine D2 receptor (D2R; RC213065) and protein kinase A catalytic subunit (PRKACA; RC210332) were used to produce target proteins for ultrasensitive multiplexed detection of cancer biomarkers.
Snippet: The pCMV6 expression plasmids containing the cDNA coding for C-term Myc-FLAG-tagged dopamine D2 receptor (D2R;catalog #RC213065) and Protein kinase A catalytic subunit (PKA Cα) (PRKACA;catalog #RC210332) were obtained from ORIGENE (Rockville, Maryland, United States)
Integrin α9 depletion promotes β-catenin degradation to suppress triple-negative breast cancer tumor growth and metastasis
Wang, Li, Xiao et al
Int. J. Cancer (2019)
Wang, Li, Xiao et al
Int. J. Cancer (2019)
AI Summary: OriGene products contributed to this study by providing full-length human ITGA9, β-catenin, and PKA-Cα cDNAs, which were used for overexpression experiments to investigate the molecular mechanisms underlying ITGA9's role in triple-negative breast cancer.
Snippet: in ITGA9 KO LM2 and SUM-159 cells Full length human ITGA9, β-catenin and PKA-Cα cDNAs were obtained from OriGene Technologies (Rockville, MD) and Addgene and cloned into lentiviral expression vector pLenti6.3⁄V5
Alterations in Protein Kinase A Substrate Specificity as a Potential Cause of Cushing Syndrome
Bathon, Weigand, Vanselow et al
Endocrinology (2019) 160 (2), 447-459 DOI: 10.1210/en.2018-00775
Bathon, Weigand, Vanselow et al
Endocrinology (2019) 160 (2), 447-459 DOI: 10.1210/en.2018-00775
AI Summary: OriGene contributed to this study by providing the plasmid encoding human wild-type Cα, which was used as a template to generate PRKACA mutants for investigating alterations in protein kinase A substrate specificity related to Cushing syndrome.
Snippet: Methods Plasmids Plasmids encoding human RIα, RIIβ and RIIβ-FLAG were described earlier (13). A plasmid encoding human wild-type Cα was purchased from Origene. Mutations in the coding sequence of Cα were introduced by PCR as previously described (10
Interaction of AIP with protein kinase A (cAMP-dependent protein kinase)
Schernthaner-Reiter, Trivellin, Stratakis
Hum. Mol. Genet. (2018)
Schernthaner-Reiter, Trivellin, Stratakis
Hum. Mol. Genet. (2018)
AI Summary: In this study, the OriGene product pCMV-PRKACA was used to investigate the interaction between AIP and the PKA catalytic subunit, contributing to the elucidation of AIP's role in PKA pathway regulation and pituitary tumorigenesis.
Snippet: 24 hours unless stated otherwise. The plasmids used were: AIP-Myc-pcDNA, AIP_R304*- Myc-pcDNA (27), pcDNA-PRKAR1A (39), pCMV-PRKACA (OriGene, Rockville, MD, USA), PRKAR1A-HA, PRKACA-HA, HSC70-HA and HSP90-HA (all cloned into the pSF
Showing 1-6 of 7 papers.
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