P3H3 (NM_014262) Human Untagged Clone
$675.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | P3H3 |
| Synonyms | GRCB; HSU47926; LEPREL2 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_014262 |
| Insert Size | 2850 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014262.2, NP_055077.2 |
| RefSeq Size | 2893 bp |
| RefSeq ORF | 2211 bp |
| Locus ID | 10536 |
| UniProt ID | Q8IVL6 |
| Cytogenetics | 12p13.31 |
| Domains | 2OG-FeII_Oxy, P4Hc |
| Summary | The protein encoded by this gene belongs to the leprecan family of proteoglycans, which function as collagen prolyl hydroxylases that are required for proper collagen biosynthesis, folding and assembly. This protein, like other family members, is thought to reside in the endoplasmic reticulum. Epigenetic inactivation of this gene is associated with breast and other cancers, suggesting that it may function as a tumor suppressor. provided by RefSeq, Aug 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014262" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215599 | LEPREL2 (Myc-DDK-tagged)-Human leprecan-like 2 (LEPREL2) | 10 ug |
$589.00
$674.00
|
|
| RC215599L3 | Lenti ORF clone of Human leprecan-like 2 (LEPREL2), Myc-DDK-tagged | 10 ug |
$974.00
|
|
| RC215599L4 | Lenti ORF clone of Human leprecan-like 2 (LEPREL2), mGFP tagged | 10 ug |
$974.00
|
|
| RG215599 | LEPREL2 (tGFP-tagged) - Human leprecan-like 2 (LEPREL2) | 10 ug |
$874.00
|
|
Citations
The prolyl 3-hydroxylases P3H2 and P3H3 are novel targets for epigenetic silencing in breast cancer
Shah, Smith, Purdie et al
Br J Cancer (2009) 100 (10), 1687-96 DOI: 10.1038/sj.bjc.6605042
Shah, Smith, Purdie et al
Br J Cancer (2009) 100 (10), 1687-96 DOI: 10.1038/sj.bjc.6605042
Cited by 74
AI Summary: In this study, OriGene products—specifically, the P3H2 (RC206541) and P3H3 (RC217999) expression plasmids—were used to restore gene expression in breast cancer cell lines, enabling functional analysis of their tumor suppressor roles following epigenetic silencing.
Showing 1-1 of 1 papers.
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