CDCA4 (NM_145701) Human Untagged Clone

SKU
SC124636
CDCA4 (untagged)-Human cell division cycle associated 4 (CDCA4), transcript variant 2
$300.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CDCA4
Synonyms HEPP; SEI-3/HEPP
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC124636 sequence for NM_145701 edited (data generated by NextGen Sequencing)
ATGTTTGCACGAGGACTGAAGAGGAAATGTGTTGGCCACGAGGAAGACGTGGAGGGAGCC
CTGGCCGGCTTGAAGACAGTGTCCTCATACAGCCTGCAGCGGCAGTCGCTCCTGGACATG
TCTCTGGTGAAGTTGCAGCTTTGCCACATGCTTGTGGAGCCCAACCTGTGCCGCTCAGTC
CTCATTGCCAACACGGTCCGGCAGATCCAAGAGGAGATGACGCAGGATGGGACGTGGCGC
ACAGTGGCACCCCAGGCTGCAGAGCGGGCGCCGCTCGACCGCTTGGTCTCCACGGAGATC
CTGTGCCGTGCAGCGTGGGGGCAAGAGGGGGCACATCCTGCTCCTGGCTTGGGGGACGGC
CACACACAGGGTCCAGTTTCTGACCTTTGCCCAGTCACCTCAGCACAGGCACCAAGGCAC
CTGCAGAGCAGCGCCTGGGAGATGGATGGCCCTCGAGAAAACAGAGGAAGCTTTCACAAG
TCACTTGATCAGATATTTGAAACGCTGGAGACTAAAAACCCCAGCTGCATGGAAGAGCTG
TTCTCAGACGTGGACAGCCCCTACTACGACCTGGACACAGTACTGACAGGCATGATGGGG
GGTGCCAGGCCGGGCCCCTGCGAAGGGCTCGAGGGCTTGGCTCCGGCCACCCCGGGCCCT
AGCTCCAGCTGCAAGTCCGACCTGGGCGAGCTGGACCACGTGGTGGAGATCCTGGTGGAG
ACCTGA

Clone variation with respect to NM_145701.2
654 a=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_145701 unedited
TTCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA
GAGCTCATTTAGCTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA
ATTCGGCACGAGGCGGCAGCGGCGGTGGCGGTGGCTGAGCAGAGGACCCGGCGGGCGGCC
TCGCGGGTCAGGACACAATGTTTGCACGAGGACTGAAGAGGAAATGTGTTGGCCACGAGG
AAGACGTGGAGGGAGCCCTGGCCGGCTTGAAGACAGTGTCCTCATACAGCCTGCAGCGGC
AGTCGCTCCTGGACATGTCTCTGGTGAAGTTGCAGCTTTGCCACATGCTTGTGGAGCCCA
ACCTGTGCCGCTCAGTCCTCATTGCCAACACGGTCCGGCAGATCCAAGAGGAGATGACGC
AGGATGGGACGTGGCGCACAGTGGCACCCCAGGCTGCAGAGCGGGCGCCGCTCGACCGCT
TGGTCTCCACGGAGATCCTGTGCCGTGCAGCGTGGGGGCAAGAGGGGGCACATCCTGCTC
CTGGCTTGGGGGACGGCCACACACAGGGTCCAGTTTCTGACCTTTGCCCAGTCACCTCAG
CACAGGCACCAAGGCACCTGCAGAGCAGCGCCTGNGAGATGGATGGCCCTCGAGAAACAG
AGGAAGCTNTCACAAGTCACTTGATCAGATATTTGAAACGCTGGAGACTAAAAACCCCAG
CTGCATGGAAGAGCTGTTCTCAGACGTGGACAGCCCCTACTACGACCTGGACACAGTACT
GACAGGCATGATGGGNGGGTGCCCAGGCCGGCCCCTGCGAAGGNCTCGAGGGCTTGGCTC
CCGCCACCCGGGCCCTACTCAGCTGCAGNTCGACTGGCGACTGACCCGTGNNTGAGATCC
TGTGAGACTGACAGAGCCTGATGCTACAGCGCTTGAGCTTGCACTGACCTGCTCCACGAG
TGCC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_145701 unedited
ATACTAGTGTACGCGGCCGCCTCNANGATCGAGTTTTTTTTTTTTTTTTTTGTTTGCTGG
ATAATTTTTTAATAAAGTGAGGTTTACATCACTGATATTTAAGAATGGCTATATGCACAA
AAGAAAACACACCTTTTTGGTTAAGGGGTGAGGAAGTTAGAGAAAGCATGAGAAACAGGG
AGCATGTGGGGTGAGGCGGGGCAGGAGTGGAAGGCTGCAGGCACCCCCAGCTCACTCCCT
GCCTGCGGCAGCACCCATGACACTACAGATCAAGGGGTTATGAATGACATGGATTCAGAT
TTCTTTCATTCTAGACTTCAACCTAGCCTTAACCTTTTGTTTCAGCACCAGTCTAACAGA
GCAGCGCAGGCGTTTCTCATCCAGCAGCAATGCTACTTCCTCACCCAGGGCAGGTGCATG
GGCTGACCCTGACGGGAGCCTCCAGAACACTGCCTTGAGAAGGCACCACCGAGGGGCTAT
GAGGTCCCTGAAGACCAACTGGTGCCTGTGATCTACAAACAGACAAATATTTGACCTTTG
TCTTCAATGACATTTACATATAAATAGACATAAATAAAATCCTCCATAATTTACTCTTAA
GTCTAGGAAGATGTGGCTGTGTGCGGTTCCTGATTTTCACCCAGGTCTCACTGCAGCGCA
GGACATANTGAAGAAGTTTCACACACACATTTTAAAGTAAACATGATACATTCACACTGG
TGACTGAGACTGTAGTGATGCTCACTGCNNAAAACAAAACAAACANAAACCCCTATTTAA
CCTTAAAAAAATGAAACACACCNACTGGCATTGGTTAAGCTATAAAAGTTCTAATGTTGC
AGCAAAGGATGTTATAGAAAAAATATACTTTTTAGAAAAATAGCTGAAAAAATTCTAAGA
GATAAGGTTA
Restriction Sites NotI-NotI
ACCN NM_145701
Insert Size 2000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145701.1, NP_663747.1
RefSeq Size 1645 bp
RefSeq ORF 726 bp
Locus ID 55038
UniProt ID Q9BXL8
Cytogenetics 14q32.33
Protein Families ES Cell Differentiation/IPS
Summary This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1. [provided by RefSeq, May 2014]
Transcript Variant: This variant (2) lacks a segment in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.
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Citations

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