CISH (NM_145071) Human Untagged Clone

SKU SC124560

CISH (untagged)-Human cytokine inducible SH2-containing protein (CISH), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: CISH
• Synonyms: BACTS2; CIS; CIS-1; G18; SOCS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124560 sequence for NM_145071 edited (data generated by NextGen Sequencing); ATGGTCCTCTGCGTTCAGGGACCTCGTCCTTTGCTGGCTGTGGAGCGGACTGGGCAGCGG; CCCCTGTGGGCCCCGTCCCTGGAACTGCCCAAGCCAGTCATGCAGCCCTTGCCTGCTGGG; GCCTTCCTCGAGGAGGTGGCAGAGGGTACCCCAGCCCAGACAGAGAGTGAGCCAAAGGTG; CTGGACCCAGAGGAGGATCTGCTGTGCATAGCCAAGACCTTCTCCTACCTTCGGGAATCT; GGCTGGTATTGGGGTTCCATTACGGCCAGCGAGGCCCGACAACACCTGCAGAAGATGCCA; GAAGGCACGTTCTTAGTACGTGACAGCACGCACCCCAGCTACCTGTTCACGCTGTCAGTG; AAAACCACTCGTGGCCCCACCAATGTACGCATTGAGTATGCCGACTCCAGCTTCCGTCTG; GACTCCAACTGCTTGTCCAGGCCACGCATCCTGGCCTTTCCGGATGTGGTCAGCCTTGTG; CAGCACTATGTGGCCTCCTGCACTGCTGATACCCGAAGCGACAGCCCCGATCCTGCTCCC; ACCCCGGCCCTGCCTATGCCTAAGGAGGATGCGCCTAGTGACCCAGCACTGCCTGCTCCT; CCACCAGCCACTGCTGTACACCTAAAACTGGTGCAGCCCTTTGTACGCAGAAGCAGTGCC; CGCAGCCTGCAACACCTGTGCCGCCTTGTCATCAACCGTCTGGTGGCCGACGTGGACTGC; CTGCCACTGCCCCGGCGCATGGCCGACTACCTCCGACAGTACCCCTTCCAGCTCTGA; ; Clone variation with respect to NM_145071.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145071 unedited; AAANGGGGAAAACAAACCCCTCCCNCNGNCCGGTTCAAATTTGTATACGACTCCTATAGG; CGGCCGCGNAATTCGCACGAGCGGNATCGCCGCTGCCGCGGGGACATGGTCCTCTGCGTT; CAGGGACCTCGTCCTTTGCTGGCTGTGGAGCGGACTGGGCAGCGGCCCCTGTGGGCCCCG; TCCCTGGAACTGCCCAAGCCAGTCATGCAGCCCTTGCCTGCTGGGGCCTTCCTCGAGGAG; GTGGCAGAGGGTACCCCAGCCCAGACAGAGAGTGAGCCAAAGGTGCTGGACCCAGAGGAG; GATCTGCTGTGCATAGCCAAGACCTTCTCCTACCTTCGGGAATCTGGCTGGTATTGGGGT; TCCATTACGGCCAGCGAGGCCCGACAACACCTGCAGAAGATGCCAGAAGGCACGTTCTTA; GTACGTGACAGCACGCACCCCAGCTACCTGTTCACGCTGTCAGTGAAAACCACTCGTGGC; CCCACCAATGTACGCATTGAGTATGCCGACTCCAGCTTCCGTCTGGACTCCAACTGCTTG; TCCAGGCCACGCATCCTGGCCTTTCCGGATGTGGTCAGCCTTGTGCAGCACTATGTGGCC; TCCTGCACTGCTGATACCCGAAGCGACAGCCCCGATCCTGCTCCCACCCCGGCCCTGCCT; ATGCCTAAGGANGATGCGCCTAGTGACCCAGCACTGCCTGCTCCTNCACCAGCCACTGCT; GTACACCTAAAACTGGTGCAGCCCTTTGTACGCAGAAGCAGTGCCCGCAGCCCTGCACAC; CTGTGCCGNCTTGTCATCAACCGTCTGGTGGCCGACGTGGACTGCCTGCCACTGGCCCGN; NGCATGGGCGACTACCTNCGAAAGTACCCCTTNCAGCTTTGACTGTACGGGGACATTTGC; CCACCCTTACCCAGNTGCACCCCTGGAGGGGAATCAACCCCAGCTGGACTGGGGNCCCCA; CTGNCCTTCTCCAGCATNCTGNNGGCTGCATACCTTGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_145071
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145071.1, NP_659508.1
• RefSeq Size: 2035 bp
• RefSeq ORF: 777 bp
• Locus ID: 1154
• UniProt ID: Q9NSE2
• Cytogenetics: 3p21.2
• Domains: SH2, SOCS
• Protein Families: Druggable Genome
• Protein Pathways: Jak-STAT signaling pathway
• Summary: The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]; Transcript Variant: This variant (2) lacks a segment in the 5' UTR and coding region compared to variant 1. The translation begins at an in-frame upstream start codon, and results in an isoform (2) with a different N-terminus, as compared to isoform 1.