CLTRN (NM_020665) Human Untagged Clone

SKU SC122895

TMEM27 (untagged)-Human transmembrane protein 27 (TMEM27)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CLTRN
• Synonyms: NX-17; NX17; TMEM27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020665 edited; GGGGCTTTTGAGGTAGAGTCAGAGTAGGGAAAACAACTAATCTGTTTTCCAAACAGGTTT; TCATAAATAACTGACAGGAGATGGGCTGTTTTTATGGAAGTATTAACCCAAATGATGGGT; GTTAATCATTAAACCTTTTTGGTGGTGGCTCGCTTGTTTCTCTGATATTCTAATCCGGGT; CACAGAATGGCTAAGGCAGATTTAAGTGCAGCGTGGATTTTTTTTTTCTCACTTTGCCTT; GTGTTTTCCACCCTGAAAGAATGTTGTGGCTGCTCTTTTTTCTGGTGACTGCCATTCATG; CTGAACTCTGTCAACCAGGTGCAGAAAATGCTTTTAAAGTGAGACTTAGTATCAGAACAG; CTCTGGGAGATAAAGCATATGCCTGGGATACCAATGAAGAATACCTCTTCAAAGCGATGG; TAGCTTTCTCCATGAGAAAAGTTCCCAACAGAGAAGCAACAGAAATTTCCCATGTCCTAC; TTTGCAATGTAACCCAGAGGGTATCATTCTGGTTTGTGGTTACAGACCCTTCAAAAAATC; ACACCCTTCCTGCTGTTGAGGTGCAATCAGCCATAAGAATGAACAAGAACCGGATCAACA; ATGCCTTCTTTCTAAATGACCAAACTCTGGAATTTTTAAAAATCCCTTCCACACTTGCAC; CACCCATGGACCCATCTGTGCCCATCTGGATTATTATATTTGGTGTGATATTTTGCATCA; TCATAGTTGCAATTGCACTACTGATTTTATCAGGGATCTGGCAACGTAGAAGAAAGAACA; AAGAACCATCTGAAGTGGATGACGCTGAAGATAAGTGTGAAAACATGATCACAATTGAAA; ATGGCATCCCCTCTGATCCCCTGGACATGAAGGGAGGGCATATTAATGATGCCTTCATGA; CAGAGGATGAGAGGCTCACCCCTCTCTGAAGGGCTGTTGTTCTGCTTCCTCAAGAAATTA; AACATTTGTTTCTGTGTGACTGCTGAGCATCCTGAAATACCAAGAGCAGATCATATATTT; TGTTTCACCATTCTTCTTTTGTAATAAATTTTGAATGTGCTTGAAAGTGAAAAGCAATCA; ATTATACCCACCAACACCACTGAAATCATAAGCTATTCACGACTCAAAATATTCTAAAAT; ATTTTTCTGACAGTATAGTGTATAAATGTGGTCATGTGGTATTTGTAGTTATTGATTTAA; GCATTTTTAGAAATAAGATCAGGCATATGTATATATTTTCACACTTCAAAGACCTAAGGA; AAAATAAATTTTCCAGTGGAGAATACATATAATATGGTGTAGAAATCATTGAAAATGGAT; CCTTTTTGACGATCACTTATATCACTCTGTATATGACTAAGTAAACAAAAGTGAGAAGTA; ATTATTGTAAATGGATGGATAAAAATGGAATTACTCATATACAGGGTGGAATTTTATCCT; GTTATCACACCAACAGTTGATTATATATTTTCTGAATATCAGCCCCTAATAGGACAATTC; TATTTGTTGACCATTTCTACAATTTGTAAAAGTCCAATCTGTGCTAACTTAATAAAGTAA; TAATCATCTCTTAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020665 unedited; CGCGGGAGTTGGGATTTGTATACGATCATATAGGCGGCCGCGCAATTCGGCCATTACGGC; CGGGGTCTTATTGAGGTAGTAGTCAGTAGTGAGGGAAAACAACTAATCTGTTTTCCAAAC; AGGTTTTCATAAATAACTGACAGGAGATGGGCTGTTTTTATGGAAGTATTAACCCAAATG; ATGGGTGTTAATCATTAAACCTTTTTGGTGGTGGCTCGCTTGTTTCTCTGATATTCTAAT; CCGGGTCACAGAATGGCTAAGGCAGATTTAAGTGCAGCGTGGATTTTTTTTTTCTCACTT; TGCCTTGTGTTTTCCACCCTGAAAGAATGTTGTGGCTGCTCTTTTTTCTGGTGACTGCCA; TTCATGCTGAACTCTGTCAACCAGGTGCAGAAAATGCTTTTAAAGTGAGACTTAGTATCA; GAACAGCTCTGGGAGATAAAGCATATGCCTGGGATACCAATGAAGAATACCTCTTCAAAG; CGATGGTAGCTTTCTCCATGAGAAAAGTTCCCAACAGAGAAGCAACAGAAATTTCCCATG; TCCTACTTTGCAATGTGACCCAGAGGGTATCATTCTGGTTTGTGGTTACAGACCCTTCAA; AAAATCACACCCTTCCTGCTGTTGAGGTGCAATCAGCCATAAAAACTGAACAGACCCGGG; TCAACAATGCCTTCTTTCTAAATGACCAAACTCTGGGATTTTTAAAAATCCCTTCCACAC; TTGCCCCAACCATGGAACCCATCTGGGGCCCATCTGGGATTATTATATTTTGGTGTGGAA; ATTTTGCAATCATCATAGTTGCAATTTGCACTACTGAATTTTATTAAGGGATCCTGGCAA; ACGTTGAAAAAAAGAAACAAAGAAACCATCTGGAAGTGGAATGAACCCTGGAAGAATAAA; TGTGGAAAACC;
• Restriction Sites: Please inquire
• ACCN: NM_020665
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020665.2, NP_065716.1
• RefSeq Size: 1605 bp
• RefSeq ORF: 669 bp
• Locus ID: 57393
• UniProt ID: Q9HBJ8
• Cytogenetics: Xp22.2
• Protein Families: Transmembrane
• Summary: This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013]