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RAG2 (NM_000536) Human Untagged Clone

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SKU
SC122564
RAG2 (untagged)-Human recombination activating gene 2 (RAG2), transcript variant 1
  $492.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RAG2
Synonyms RAG-2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_000536 edited
ACTCTCTTTACAGTCAGCCTTCTGCTTGCCACAGTCATAGTGGGCAGTCAGTGAATCTTC
CCCAAGTGCTGACAATTAATACCTGGTTTAGCGGCAAAGATTCAGAGAGGCGTGAGCAGC
CCCTCTGGCCTTCAGACAAAAATCTACGTACCATCAGAAACTATGTCTCTGCAGATGGTA
ACAGTCAGTAATAACATAGCCTTAATTCAGCCAGGCTTCTCACTGATGAATTTTGATGGA
CAAGTTTTCTTCTTTGGACAAAAAGGCTGGCCCAAAAGATCCTGCCCCACTGGAGTTTTC
CATCTGGATGTAAAGCATAACCATGTCAAACTGAAGCCTACAATTTTCTCTAAGGATTCC
TGCTACCTCCCTCCTCTTCGCTACCCAGCCACTTGCACATTCAAAGGCAGCTTGGAGTCT
GAAAAGCATCAATACATCATCCATGGAGGGAAAACACCAAACAATGAGGTTTCAGATAAG
ATTTATGTCATGTCTATTGTTTGCAAGAACAACAAAAAGGTTACTTTTCGCTGCACAGAG
AAAGACTTGGTAGGAGATGTTCCTGAAGCCAGATATGGTCATTCCATTAATGTGGTGTAC
AGCCGAGGGAAAAGTATGGGTGCTCTCTTTGGAGGACGCTCATACATGCCTTCTACCCAC
AGAACCACAGAAAAATGGAATAGTGTAGCTGACTGCCTGCCCTGTGTTTTCCTGGTGGAT
TTTGAATTTGGGTGTGCTACATCATACATTCTTCCAGAACTTCAGGATGGGCTATCTTTT
CATGTCTCTATTGCCAAAAATGACACCATCTATATTTTAGGAGGACATTCACTTGCCAAT
AATATCCGGCCTGCCAACCTGTACAGAATAAGGGTTGATCTTCCCCTGGGTAGCCCAGCT
GTGAATTGCACAGTCTTGCCAGGAGGAATCTCTGTCTCCAGTGCAATCCTGACTCAAACT
AACAATGATGAATTTGTTATTGTTGGTGGCTATCAGCTTGAAAATCAAAAAAGAATGATC
TGCAACATCATCTCTTTAGAGGACAACAAGATAGAAATTCGTGAGATGGAGACCCCAGAT
TGGACCCCAGACATTAAGCACAGCAAGATATGGTTTGGAAGCAACACGGGAAATGGAACT
GTTTTTCTTGGCATACCAGGAGACAATAAACAAGTTGTTTCAGAAGGATTCTATTTCTAT
ATGTTGAAATGTGCTGAAGATGATACTAATGAAGAGCAGACAACATTCACAAACAGTCAA
ACATCAACAGAAGATCCAGGGGATTCCACTCCCTTTGAAGACTCTGAAGAATTTTGTTTC
AGTGCAGAAGCAAATAGTTTTGATGGTGATGATGAATTTGACACCTATAATGAAGATGAT
GAAGAAGATGAGTCTGAGACAGGCTACTGGATTACATGCTGCCCTACTTGTGATGTGGAT
ATCAACACTTGGGTACCATTCTATTCAACTGAGCTCAACAAACCCGCCATGATCTACTGC
TCTCATGGGGATGGGCACTGGGTCCATGCTCAGTGCATGGATCTGGCAGAACGCACACTC
ATCCATCTGTCAGCAGGAAGCAACAAGTATTACTGCAATGAGCATGTGGAGATAGCAAGA
GCTCTACACACTCCCCAAAGAGTCCTACCCTTAAAAAAGCCTCCAATGAAATCCCTCCGT
AAAAAAGGTTCTGGAAAAATCTTGACTCCTGCCAAGAAATCCTTTCTTAGAAGGTTGTTT
GATTAGTTTTGCAAAAGCCTTTCAGATTCAGGTGTATGGAATTTTTGAATCTATTTTTAA
AATCATAACATTGATTTTAAAAATACATTTTTGTTTATTTAAAATGCCTATGTTTTCTTT
TAGTTACATGAATTAAGGGCCAGAAAAAAGTGTTTATAATGCAATGATAAATAAAGTCAT
TCTAGACCCTATACATTTTGAAAATATTTTACCCAAATACTCAATTTACTAATTTATTCT
TCACTGAGGATTTCTGATCTGATTTTTTATTCAACAAACCTTAAACACCCAGAAGCAGTA
ATAATCATCGAGGTATGTTTATATTTATTATATGAGTCTTGGTAACAAATAACCTATAAA
GTGTTTATGACAAATTTAGCCAATAAAGAAATTAACACCCAAAAGAATTAAATTGATTAT
TTTGTGCAACATAACAATTCGGCAGTTGGCCAAAACTTAAAAGCAAGATCTACTACATCC
CACATTAGTGTTCTTTATATACCTTCAAGCAACCCTTTGGATTATGCCCATGAACAAGTT
AGTTTCTCATAGCTTTACAGATGTAGATATAAATATAAATATATGTATACATATAGATAG
ATAATGTTCTCCACTGACACAAAAGAAGAAATAAATAATCTACATCAAAAAAAAAAAAAA
AAAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000536 unedited
GGTCAAAATTTGTATACGACTCATATGGGCGGCNCGCGAATCGGCCATTACGGCCGGGGA
CTCTCTTTACAGTCAGCCTTCTGCTTGCCACAGTCATAGTGGGCAGTCAGTGAATCTTCC
CCAAGTGCTGACAATTAATACCTGGTTTAGCGGCAAAGATTCAGAGAGGCGTGAGCAGCC
CCTCTGGCCTTCAGACAAAAATCTACGTACCATCAGAAACTATGTCTCTGCAGATGGTAA
CAGTCAGTAATAACATAGCCTTAATTCAGCCAGGCTTCTCACTGATGAATTTTGATGGAC
AAGTTTTCTTCTTTGGACAAAAAGGCTGGCCCAAAAGATCCTGCCCCACTGGAGTTTTCC
ATCTGGATGTAAAGCATAACCATGTCAAACTGAAGCCTACAATTTTCTCTAAGGATTCCT
GCTACCTCCCTCCTCTTCGCTACCCAGCCACTTGCACATTCAAAGGCAGCTTGGAGTCTG
AAAAGCATCAATACATCATCCATGGAGGGAAAACACCAAACAATGAGGTTTCAGATAAGA
TTTATGTCATGTCTATTGTTTGCAAGAACAACAAAAAGGTTACTTTTCGCTGCACAGAGA
AAGACTTGGTAGGAGATGTTCCTGAAGCCAGATATGGTCATTCCATTAATGTGGTGTACA
GCCGAGGGAAAAGTATGGGTGCTCTCTTTGGAGGACGCTCATACATGCCTTCTACCCACA
GAAACCACAGAAAATGGAATAGTGTAGCTGACTGCCTGCCCTGTGTTTTCCTGGTGGGAT
TTGAATTTGGGTGTGCTACATCATACATTTCTTCAGAACTTCANGATGGGCTATCTTTTC
ATGTCTCTATTGCCAANATGACACATCTATATTTTAGGAGGACATTCACTGCCAATAATA
TCCGGCCTGCCAACCTGTACAGAATAAGGA
Restriction Sites Please inquire |
ACCN NM_000536
Insert Size 2414 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000536.1, NP_000527.1
RefSeq Size 2414 bp
RefSeq ORF 1584 bp
Locus ID 5897
UniProt ID P55895
Cytogenetics 11p12
Protein Families Druggable Genome
Protein Pathways Primary immunodeficiency
Summary This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 3 and 4 encode the same protein.
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Documents
Resources
"NM_000536" in other vectors (6)
SKU Description Size Price
RC205065 RAG2 (Myc-DDK-tagged)-Human recombination activating gene 2 (RAG2), transcript variant 1 10 ug
$491.00
RC205065L1 Lenti ORF clone of Human recombination activating gene 2 (RAG2), transcript variant 1, Myc-DDK-tagged 10 ug
$791.00
RC205065L2 Lenti ORF clone of Human recombination activating gene 2 (RAG2), transcript variant 1, mGFP tagged 10 ug
$791.00
RC205065L3 Lenti ORF clone of Human recombination activating gene 2 (RAG2), transcript variant 1, Myc-DDK-tagged 10 ug
$791.00
RC205065L4 Lenti ORF clone of Human recombination activating gene 2 (RAG2), transcript variant 1, mGFP tagged 10 ug
$791.00
RG205065 RAG2 (tGFP-tagged) - Human recombination activating gene 2 (RAG2), transcript variant 1 10 ug
$489.00 $691.00

file-alt Citations

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