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PNPLA4 (NM_004650) Human Untagged Clone

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SKU
SC121700
PNPLA4 (untagged)-Human patatin-like phospholipase domain containing 4 (PNPLA4), transcript variant 1
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PNPLA4
Synonyms DXS1283E; GS2; iPLA2eta
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC121700 sequence for NM_004650 edited (data generated by NextGen Sequencing)
ATGAAGCACATCAACCTATCATTTGCAGCGTGTGGATTTCTGGGCATTTACCACTTGGGG
GCAGCATCTGCACTTTGCAGACATGGCAAAAAACTTGTGAAGGATGTCAAAGCCTTCGCT
GGGGCGTCTGCGGGATCGTTGGTTGCTTCTGTTCTGCTAACAGCACCAGAAAAAATAGAG
GAATGTAACCAATTTACCTACAAGTTTGCCGAAGAAATCAGAAGGCAGTCTTTCGGGGCA
GTAACGCCCGGTTATGACTTCATGGCCCGACTAAGAAGTGGGATGGAGTCGATTCTTCCT
CCCAGCGCTCACGAGCTGGCCCAGAACCGACTGCACGTATCCATCACCAACGCCAAAACC
AGAGAAAATCACTTAGTCTCCACTTTTTCCTCCAGGGAGGACCTCATTAAGGTCCTCCTA
GCCAGCAGTTTTGTGCCCATTTATGCAGGACTGAAGCTAGTGGAATACAAAGGGCAGAAG
TGGGTGGACGGAGGCCTCACCAACGCTCTTCCCATCCTGCCCGTCGGCCGGACAGTAACC
ATCTCCCCCTTCAGTGGACGACTGGACATCTCCCCGCAGGACAAAGGGCAGCTAGATCTG
TATGTTAATATCGCCAAGCAGGATATCATGTTGTCCCTGGCAAACCTGGTGAGACTCAAC
CAAGCCCTTTTTCCCCCAAGCAAGAGGAAAATGGAATCTTTGTATCAGTGTGGTTTTGAT
GACACTGTTAAGTTTTTACTTAAAGAAAATTGGTTTGAATAA

Clone variation with respect to NM_004650.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004650 unedited
TTGTAATACGACTCACTTAGGGCGGCCGCGACTTCGGCACGAGGCAGGACTTTCCTGGGC
CGGCTCGCGGAGAGCGTAGCGCGGCCTTGGTGGCGGAATGGCGTTGAGTGACGGCCCGGC
CCCGCCATCTGGTTAAAGGGACTCGTTCACACGGAAGTGTCCCGGGGCTGCATTGTGCTA
CAGCTAGAATGAAGCACATCAACCTATCATTTGCAGCGTGTGGATTTCTGGGCATTTACC
ACTTGGGGGCAGCATCTGCACTTTGCAGACATGGCAAAAAACTTGTGAAGGATGTCAAAG
CCTTCGCTGGGGCGTCTGCGGGATCGTTGGTTGCTTCTGTTCTGCTAACAGCACCAGAAA
AAATAGAGGAATGTAACCAATTTACCTACAAGTTTGCCGAAGAAATCAGAAGGCAGTCTT
TCGGGNGCAGTAACGCCCGGTTATGACTTCATGGCCCACTAAGAAGTGGGAAGGGAGTCG
ATTCTTCCTCCCAGCGCTCACGAGCTGGCCCAGAACCGACTGCACGTATCCATCACCAAC
GCCAAAACCAGAGAAAATCACTTAGTCTCCACTTTTCCTTNGGGGGGAAGACCTCATTTT
AGGTCCTCTACCCANCAAGGTTTTTGTGCCCATTNATGCANGACTGAAGCTAGTGGATAC
AAGGCCAAAGTGGGGGGGACACGGAGCCTCACCACGCTCTTCCATNCCTGCCGTCGGCCG
GACAGTACCATCTCCCCTTCAGTGGACGACTGGACATCTCCCCGCAGGACAAGGCAGCTA
ATTTNTGTATGTTATATCGCCAGCAGATATAAAGGGTTGTCCTGGCAAACCTGGTGAGAT
CAACCCAGCCTTTTTCCCCAGCAGAAGAAATGGATCTTGTTANNNNNGGGGTTTTTAAAA
CTTTGTAAAC
Restriction Sites NotI-NotI |
ACCN NM_004650
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004650.1, NP_004641.1
RefSeq Size 2809 bp
RefSeq ORF 762 bp
Locus ID 8228
UniProt ID P41247
Cytogenetics Xp22.31
Protein Pathways Retinol metabolism
Summary This gene encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome Y. provided by RefSeq, Feb 2010
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.
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Documents
Resources
"NM_004650" in other vectors (5)
SKU Description Size Price
RC204732 PNPLA4 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 4 (PNPLA4), transcript variant 1 10 ug
$300.00
RC204732L3 Lenti ORF clone of Human patatin-like phospholipase domain containing 4 (PNPLA4), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC204732L4 Lenti ORF clone of Human patatin-like phospholipase domain containing 4 (PNPLA4), transcript variant 1, mGFP tagged 10 ug
$600.00
RG204732 PNPLA4 (tGFP-tagged) - Human patatin-like phospholipase domain containing 4 (PNPLA4), transcript variant 1 10 ug
$489.00 $500.00
SC324530 PNPLA4 (untagged)-Human patatin-like phospholipase domain containing 4 (PNPLA4), transcript variant 1 10 ug
$300.00

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